Optical Sensing of Red Blood Cell Dynamics

Park, YongKeun; Best, Catherine A.; Popescu, Gabriel

doi:10.1007/978-1-4419-8083-0_13

Cited by 13 publications

(13 citation statements)

References 103 publications

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“…QPI techniques based on a common-path interferometry design have recently achieved extremely high phase-sensitivity [19][20][21][22][23][24]; sensitivity of optical path lengths of about a few milliradian can be realized, enabling quantification of subtle cell membrane motions [25,26]. One the other hand, tomographic QPI techniques have been employed to measure the 3D tomograms of individual biological samples [27][28][29][30][31][32][33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells

Kim¹,

Shim²,

K³

et al. 2014

Opt. Express

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117

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We present an optical holographic micro-tomographic technique for imaging both the three-dimensional structures and dynamics of biological cells. Optical light field images of a sample, illuminated by a plane wave with various illumination angles, are measured in a common-path interferometry, and thus both the three-dimensional refractive index tomogram and two-dimensional dynamics of live biological cells are measured with extremely high sensitivity. The applicability of the technique is demonstrated through quantitative and measurements of morphological, chemical, and mechanical parameters at the individual cell level.

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Section: Introductionmentioning

confidence: 99%

Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells

Kim¹,

Shim²,

K³

et al. 2014

Opt. Express

Self Cite

117

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“…Recently, a dynamic SPM equipped with multiple coherent laser sources and a color-image sensor has been used to demonstrate the dynamic quantification of Hb inside individual RBCs as well as to probe the dynamic membrane fluctuations of RBCs [71]. The dynamic membrane fluctuation in cells can be related to the mechanical properties of the cell membrane cortex [90][91][92][93][94][95][96] or the label-free imaging of membrane potential [57,97].…”

Section: B Application In Cell Studymentioning

confidence: 99%

Biomedical applications of holographic microspectroscopy [Invited]

Jung

et al. 2014

Appl. Opt.

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The identification and quantification of specific molecules are crucial for studying the pathophysiology of cells, tissues, and organs as well as diagnosis and treatment of diseases. Recent advances in holographic microspectroscopy, based on quantitative phase imaging or optical coherence tomography techniques, show promise for label-free noninvasive optical detection and quantification of specific molecules in living cells and tissues (e.g., hemoglobin protein). To provide important insight into the potential employment of holographic spectroscopy techniques in biological research and for related practical applications, we review the principles of holographic microspectroscopy techniques and highlight recent studies.

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“…For example, the RBC membrane cortex, composed of lipid bilayer, cytoskeleton and junctional protein complexes, exhibits dynamic fluctuations, also called “flickering.” Dynamic fluctuations in RBCs membrane had been an intriguing research topic in soft matter physics and hematology [99] after their first observation [100], and they have shown strong correlation with the pathophysiological states of the cell [99]. QPI is an ideal tool to probe the dynamic membrane fluctuations in RBCs since the phase images of RBCs can be directly and quantitatively translated into height map, measured at high speed [77,101,102].…”

Section: Study Of Cell Physiology Using Qpimentioning

confidence: 99%

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

Lee

Jung

et al. 2013

Sensors

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484

290

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A cellular-level study of the pathophysiology is crucial for understanding the mechanisms behind human diseases. Recent advances in quantitative phase imaging (QPI) techniques show promises for the cellular-level understanding of the pathophysiology of diseases. To provide important insight on how the QPI techniques potentially improve the study of cell pathophysiology, here we present the principles of QPI and highlight some of the recent applications of QPI ranging from cell homeostasis to infectious diseases and cancer.

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Optical Sensing of Red Blood Cell Dynamics

Cited by 13 publications

References 103 publications

Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells

Common-path diffraction optical tomography for investigation of three-dimensional structures and dynamics of biological cells

Biomedical applications of holographic microspectroscopy [Invited]

Quantitative Phase Imaging Techniques for the Study of Cell Pathophysiology: From Principles to Applications

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