Optically Coated Mirror‐Embedded Microchannel to Measure Hydrophoretic Particle Ordering in Three Dimensions

Choi, Sungyoung; Park, Je‐Kyun

doi:10.1002/smll.200900799

Cited by 21 publications

(19 citation statements)

References 31 publications

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“…3 and S6). This means that the steric collisions clearly occurred in the sorting channel as experimentally observed before 16 and induced effective tethering of target cells on the sorting ridges. In the upstream focusing channel, all the cells are focused and assume similar trajectories (Fig.…”

supporting

confidence: 60%

“…1). 16 The slant ridges superpose a circulating flow pattern on the axial flow. In the trenches between the ridges, the flow circulates towards the sorting/gutter side of the channel, whereas in the gap above the top surface of the ridges, the flow re-circulates back towards the focusing side of the channel (Fig.…”

mentioning

confidence: 99%

“…This effect is called hydrophoresis. 16 However, in the presence of adhesion molecules on the surfaces, target cells are forced into contact with the ridges, and as aconsequence, tether ( i.e . initiate molecular interactions) and roll on the top surface of the ridges.…”

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confidence: 99%

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Cell sorting by deterministic cell rolling

2012

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confidence: 60%

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confidence: 99%

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Cell sorting by deterministic cell rolling

2012

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“…Choi et al 4 previously developed a hydrodynamic filtration technique (hydrophoresis) that allowed for cell positioning and filtration only in a range of lower flow rates, requiring a high precision syringe pump for device operation. Choi et al 4 previously developed a hydrodynamic filtration technique (hydrophoresis) that allowed for cell positioning and filtration only in a range of lower flow rates, requiring a high precision syringe pump for device operation.…”

Section: Microfluidic Pipette Tip For Combined Solution Transfer and mentioning

confidence: 99%

Research highlights: cell separation at the bench and beyond

et al. 2015

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We highlight recent progress in applying micro- and nanotechnology enabled cell separations to life sciences and clinical use. Microfluidic systems operate on a scale that matches that of cells (10-100 μm) and therefore allow interfacing and separations that are sensitive at this scale. Given the corresponding dimensions, it is not surprising that a wide array of microfluidic cell separation technologies have been developed using hydrodynamic, electrical, magnetic and optical forces, and have been applied to a range of biological and clinical problems in sample preparation. Passive separation approaches have distinct advantages for point of care applications or when downstream cell-based therapies are envisioned. We highlight a recent approach that allows for passive hydrodynamic filtering of cells over almost two orders of magnitude in flow conditions, which allowed the researchers to interface with a standard manual pipettor, creating a "microfluidic pipette tip". In a second work, passive separation by size yields distinct populations of mesenchymal stem cells that can be used therapeutically. The researchers report on other biophysical separations that would be expected to refine these cell populations further for the most efficacious cell-based therapies. In an intriguing twist, we highlight a creative idea in which stem cell populations could potentially also be extracted from a patient with less invasive surgeries, performing the separation using magnetic nanoparticles in vivo without bulk tissue disruption. New cell separation technologies will continue to be demonstrated, however, a major research thrust appears to be now developing these technologies to address unique application niches in point-of-care sample preparation for research and diagnostics or cell-based therapies.

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“…In the absence of adhesion interactions (BSA control), the physical interactions between the cells and the ridges force the cells to remain above the ridges where the flow converges toward the focusing side of the channel (Figure 2a and S3, top). 29,30,32 Transient ligand-receptor interactions occur when the surfaces are coated with P-selectin, which mediates cell rolling along the AR, until they are detached in the gutter region (Figure 2a). In this case, the cell trajectories follow similar paths as the streamlines inside the trenches.…”

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confidence: 99%