Optimal conditions and comparison of lactate dehydrogenase catalysis of the lactate-to-pyruvate and pyruvate-to-lactate reactions in human serum at 25, 30, and 37 degrees C.

Buhl, Steven N.; Jackson, K Y

doi:10.1093/clinchem/24.5.828

Cited by 161 publications

(63 citation statements)

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“…Spectrophotometric diagnostic kits were used for the estimation of AST, LDH and CK-MB activities, [16][17][18] as well as TG 19 and TC 20 levels. Serum enzyme activity and lipid profile were expressed as U l -1 and mg dl -1 respectively.…”

Section: Estimation Of Cardiac Function Enzymes Activities Tg and Tcmentioning

confidence: 99%

Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicin‐induced cardiac injury in rats

El-Boghdady

2012

Cell Biochemistry & Function

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Grape seed proanthocyanidins (GSPE) and ginkgo biloba extract (EGb761) are considered to have protective effects against several diseases. The cardiotoxicity of doxorubicin (DOX) has been reported to be associated with oxidative damage. This study was conducted to evaluate the cardioprotective effects of GSPE and EGb761 against DOX-induced heart injury in rats. DOX was administered as a single i.p. dose (20 mg kg(-1)) to adult male rats. DOX-intoxicated rats were orally administered GSPE (200 mg kg(-1) day(-1)) or EGb761 (100 mg kg(-1) day(-1)) for 15 consecutive days, starting 10 days prior DOX injection. DOX-induced cardiotoxicity was evidenced by a significant increase in serum aspartate transaminase (AST), creatine phosphokinase isoenzyme (CK-MB), lactate dehydrogenase (LDH), total cholesterol (TC) and triglyceride (TG) activities and levels. Increased oxidative damage was expressed by the depletion of cardiac reduced glutathione (GSH), elevation of cardiac total antioxidant (TAO) level and accumulation of the lipid peroxidation product, malondialdehyde (MDA). Significant rises in cardiac tumour necrosis factor-alpha (TNF-α) and caspase-3 levels were noticed in DOX-intoxicated rats. These changes were ameliorated in the GSPE and EGb761-treated groups. Histopathological analysis confirmed the cardioprotective effects of GSPE and EGb761. In conclusion, GSPE and EGb761 mediate their protective effect against DOX-induced cardiac injury through antioxidant, anti-inflammatory and antiapoptotic mechanisms.

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Section: Estimation Of Cardiac Function Enzymes Activities Tg and Tcmentioning

confidence: 99%

Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicin‐induced cardiac injury in rats

El-Boghdady

2012

Cell Biochemistry & Function

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“…Sorbitol dehydrogenase (SDH) activity was assayed by using the method of Chauncey et al [35]. Lactate dehydrogenase (LDH) activity was measured by using commercially available kit provided by Stanbio (San Antonio, TX), according to the method of Buhl and Jackson [36]. The SDH assay is based on interconversion of D-sorbitol and D-fructose, and the LDH assay is based on the interconversion of pyruvate and lactate.…”

Section: Testicular Marker Enzymesmentioning

confidence: 99%

Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium‐treated male rats

Sadik

2008

J Biochem & Molecular Tox

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Cadmium (Cd) is one of the environmental pollutants that affect various tissues and organs including testis. Harmful effect of cadmium on testis is known to be germ cell degeneration and impairment of testicular steroidogenesis. In the present study, the effect of diallyl sulfide (DAS), a sulfur-containing volatile compound present in garlic, and zinc (Zn) was investigated on cadmium-induced testicular toxicity in rats. Male adult Wistar rats treated with cadmium (2.5 mg/kg body wt, five times a week for 4 weeks) showed decreased body weight, paired testicular weight, relative testicular weight, serum testosterone, luteinizing hormone, follicle-stimulating hormone, and testicular total antioxidant capacity (TAC) and protein levels. Testicular steroidogenic enzymes, such as 3beta-hydroxysteroid dehydrogenase (3beta-HSD) and 17beta-hydroxysteroid dehydrogenase (17beta-HSD), and marker enzymes, such as sorbitol dehydrogenase (SDH), lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (ALP), and glucose-6-phosphate dehydrogenase (G6PD), showed a significant decrease in activities whereas that of gamma-glutamyl transferase was significantly increased after cadmium exposure. The results have revealed that concurrent treatment with DAS or zinc restored key steroidogenic enzymes, SDH, LDH, and G6PD and increased testicular weight significantly. DAS restored the TAC level and increased testosterone level and relative testicular weight significantly. Zinc restored testicular protein level and body weight. It can be concluded that cadmium causes testicular toxicity and inhibits androgen production in adult male rats probably by affecting pituitary gonadotrophins and that concurrent administration of DAS or zinc provides protection against cadmium-induced testicular toxicity.

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“…The supernatant was used for determination of the leakage of ALT, AST and LDH. [24,25] The other sample was centrifuged at 2000 rev/min (4°C) for 15 min, after addition of 200 ml of 1% Triton X, to obtain the lysate. The lysate was used for the determination of total enzyme activity in the cells.…”

Section: Determination Of Enzyme Leakagementioning

confidence: 99%

<I>Rubus sanctus</I> protects against carbon tetrachloride-induced toxicity in rat isolated hepatocytes: isolation and characterization of its galloylated flavonoids

Badr¹,

El‐Demerdash²,

Khalifa³

et al. 2009

j pharm pharmacol

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Optimal conditions and comparison of lactate dehydrogenase catalysis of the lactate-to-pyruvate and pyruvate-to-lactate reactions in human serum at 25, 30, and 37 degrees C.

Cited by 161 publications

References 0 publications

Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicin‐induced cardiac injury in rats

Antioxidant and antiapoptotic effects of proanthocyanidin and ginkgo biloba extract against doxorubicin‐induced cardiac injury in rats

Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium‐treated male rats

<I>Rubus sanctus</I> protects against carbon tetrachloride-induced toxicity in rat isolated hepatocytes: isolation and characterization of its galloylated flavonoids

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