Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology

Li, Chan; Bai, Jinghua; Cai, Zhaoling; Ouyang, Fan

doi:10.1016/s0168-1656(01)00377-7

Cited by 206 publications

(159 citation statements)

References 16 publications

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“…In general there is no defined medium designed for the production of alkaline protease from different microbial sources [18,22,23]. Every organism is unique in its requirement for the mentioned parameters for maximum enzyme production.…”

Section: Optimization By Response Surface Methodsologymentioning

confidence: 99%

Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Tarı

Gençkal

Tokatlı

2006

Process Biochemistry

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Bacillus sp. L21 was isolated from the by-products of a leather factory (located in Izmir, Turkey) working under extreme alkaline conditions. Its phenotypic and genotypic identifications were completed, and determined as a potential alkaline protease producer. After screening various elements, carbon and nitrogen sources, soybean meal, maltose50, tween80 and the initial pH conditions were chosen as main factors to be used in the experimental design and response surface methodology (RSM) for the optimization of a low cost enzyme producing media for potential use on an industrial scale. The optimized values obtained by the statistical analysis showed that soybean meal at 3.0 g/l, maltose50 between the ranges of 30 and 40 g/l, tween80 at 0.35 g/l and an initial pH of 8.0 gives maximum protease activity. #

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Section: Optimization By Response Surface Methodsologymentioning

confidence: 99%

Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Tarı

Gençkal

Tokatlı

2006

Process Biochemistry

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“…In order to attain the optimum response, several approaches have been developed, thus, most authors have used the central composite design [22]. However, central composite designs usually have axial points outside the "cube" (unless alpha, the axial spacing needed to ensure orthogonality, is specified as less than or equal to one).…”

Section: Introductionmentioning

confidence: 99%

Automatic on line preconcentration and determination of lead in water by ICP-AES using a TS-microcolumn

Zougagh

Torres

Alonso

et al. 2004

Talanta

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A simple, sensitive, low-cost and rapid, flow injection system for the on-line preconcentration of lead by sorption on a microcolumn packed with silica gel funtionalized with methylthiosalicylate (TS-gel) was developped. The metal is directly retained on the sorbent column and subsequently then eluted from it by EDTA. Five variables (sample flow rate, eluent flow rate, eluent concentration, pH and buffer concentration) were considered as factors in the optimization process. Interactions between analytical factors and their optimal levels were investigated using two level factorial and Box-Behnken designs. The optimum conditions established were applied to the determination of lead by flow injection inductively coupled plasma atomic emission spectrometry (FI-ICP-AES). The proposed method has a linear calibration range from 10 to at least 500 ng ml −1 of lead. At a sample frequency of 24 h −1 and a 120 s preconcentration time, the enrichment factor was 41, the detection limit was 15.3 ng ml −1 (S/N = 3) and the precision, expressed as relative standard deviation, was 0.9% (at 100 ng ml −1 ). Validation of the developed method was carried out against electrothermal atomic absorption spectrometry analysis without statistically significant differences between the proposed method and the atomic absorption method.

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“…Lactic acid bacterium traditionally requires complex nutrition for the growth. The MRS supplies this condition and is good medium to neutralize the lactic acid produced and improve the cell density (Li et al, 2002). However, the amounts of antimicrobial compounds in the fermentation supernatants are normally low and require studies to increase the production and concentrate the extract by purification protocols.…”

Section: Resultsmentioning

confidence: 99%

“…However, the sources studied in this work such peptone, yeast extract or meat extract, might contain proteases that would degrade the proteinaceus nature of antimicrobial substance. Moreover, the supplementation of the rich organic sources increases the cost of production and also brings difficulties in their purification (Li et al, 2002). Therefore, in this study, the influence on antimicrobial activity by the additional source of nitrogen was evaluated using urea.…”

Section: Resultsmentioning

confidence: 99%

Production biomolecule with inhibitory activity against Gram-negative bacteria isolated from faeces of broilers and swine

Bordignon

Miyaoka

Spier

et al. 2011

Braz. arch. biol. technol.

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Optimization of a cultural medium for bacteriocin production by Lactococcus lactis using response surface methodology

Cited by 206 publications

References 16 publications

Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Optimization of a growth medium using a statistical approach for the production of an alkaline protease from a newly isolated Bacillus sp. L21

Automatic on line preconcentration and determination of lead in water by ICP-AES using a TS-microcolumn

Production biomolecule with inhibitory activity against Gram-negative bacteria isolated from faeces of broilers and swine

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