Optimization of a protective medium for enhancing the viability of freeze-dried Bacillus amyloliquefaciens B1408 based on response surface methodology

Han, Lingjuan; Pu, Taixun; Wu, Xi; Liu, Beiling; Wang, Yonghong; Feng, Juntao; Xing, Zhang

doi:10.1016/j.cryobiol.2018.02.003

Cited by 27 publications

(8 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Box Behncken Design (BBD) with three factors (skim milk: X 1 , sucrose: X 2 , and trehalose: X 3 ) and the software Design Expert 8.06 (Stat-ease Inc., Minneapolis, MN, USA) was used to analyze the survival data. The analytical procedure was referred to a study with minor modification [25]. A three-variable BBD with six replicates at the center point was selected to build the response surface models.…”

Section: Methodsmentioning

confidence: 99%

“…Due to this, a variety of cryo-protectants have been developed to increase the viability of probiotic bacteria during the freeze-drying procedure [22,23]. Protectants such as skim milk, whey proteins, sugars, or other bio-polymers were studied mostly as combinations for synergistic protective effects with other protectants [24,25]. The classical one-variable-at-a-time approach (OVAT) strategy was deemed more time-consuming.…”

Section: Introductionmentioning

confidence: 99%

“…It ignores the interaction between functioning factors, which might lead to the confusion and bias of results [26]. Thus, the response surface method has become one of the most used optimization approaches to create the best conditions with a minimum number of experiments [25]. Among different optimization procedures, the Box-Behnken Design (BBD) has been shown to be superior to 3-level full factorial designs and is, thus, being used in response surface modelling [27,28].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

2019

View full text Add to dashboard Cite

In animal nutrition, probiotics are considered as desirable alternatives to antibiotic growth promoters. The beneficial effects of probiotics primarily depend on their viability in feed, which demands technical optimization of biomass production, since processing and storage capacities are often strain-specific. In this study, we optimized the production parameters for two broiler-derived probiotic lactobacilli (L. salivarius and L. agilis). Carbohydrate utilization of both strains was determined and preferred substrates that boosted biomass production in lab-scale fermentations were selected. The strains showed good aerobic tolerance, which resulted in easier scale-up production. For the freeze-drying process, the response surface methodology was applied to optimize the composition of cryoprotective media. A quadratic polynomial model was built to study three protective factors (skim milk, sucrose, and trehalose) and to predict the optimal working conditions for maximum viability. The optimal combination of protectants was 0.14g/mL skim milk/ 0.08 g/mL sucrose/ 0.09 g/mL trehalose (L. salivarius) and 0.15g/mL skim milk/ 0.08 g/mL sucrose/ 0.07 g/mL (L. agilis), respectively. Furthermore, the in-feed stabilities of the probiotic strains were evaluated under different conditions. Our results indicate that the chosen protectants exerted an extensive protection on strains during the storage. Although only storage of the strains at 4 °C retained the maximum stability of both Lactobacillus strains, the employed protectant matrix showed promising results at room temperature.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

2019

View full text Add to dashboard Cite

show abstract

“…Apart from carriers, stabilizers such as sodium benzoate or lactose are added to the formulation along with additional nutrients (glucose, molasses, peptone), thickeners (xanthan gum), surfactants, desiccants (silica gel) and many other substances that aim to make the product more durable and more efficient [124]. In a previous study it was found that addition of adjuvants, additives and surfactants enhanced the shelf-life and efficacy of Bacillus meghaterium, concluding that the combination of polyvinyl pyrrolidone, carboxymethyl cellulose and polysorbate provides the best results in liquid formulations [70].…”

Section: Formulation Proceduresmentioning

confidence: 99%

Bacillus based microbial formulations: Optimization of the production process

Stojanović

Karabegović

Beškoski

et al. 2019

Hem Ind

View full text Add to dashboard Cite

Bacillus sp.-based microbial formulations have found wide application in many fields: from pharmacy and medicine to environmental protection and agriculture due to the ability of this species to produce various metabolites and to form endospores. Recently, these products have gained popularity as biopesticidal and phytostimulatory agents, which are a ?green? alternative to overused agrochemicals. In order to obtain a high-quality and long-lasting product with desired characteristics, it is necessary to optimize the production process at each stage, which implies coordinating the microbial species, the type and the conditions of microbial cultivation along with formulation technologies. This paper provides a concise overview of the most important findings in this area, regarding characteristics of microbial formulations and specific criteria that need to be met when such a product is formulated. It should serve as a beginning point for everyone starting new research, not just in the field of biofertilization and biological control of plant diseases, but generally in the field of biochemical engineering. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 43004]

show abstract

“…The mechanism likely involves the production of various antimicrobial compounds, competition for nutrients and space, and increase the plant resistance [15, 16]. In addition, disease reduction has been attributed by promoting the growth of plant beneficial micro-organisms in the rhizosphere, thereby it inhibit the growth and survival of pathogens and increase the soil enzyme activity [14, 17–19].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity

2019

View full text Add to dashboard Cite

BackgroundThe consequence of simultaneous and sequential inoculation of T. asperellum and B. amyloliquefaciens cultures with respect to growth rate, differential expression of vital genes and metabolites were examined.ResultsThe competition was observed between T. asperellum and B. amyloliquefaciens under co-cultivation. The proliferation of Trichoderma was reduced in the simultaneous inoculation (TB1) method, possibly due to the fastest growth of Bacillus. Both T. asperellum and B. amyloliquefaciens were proliferated in sequential inoculation method (TB2). The sequential inoculation method (TB2) upregulated the expression of metabolites and vital genes (sporulation, secondary metabolites, mycoparasitism enzymes and antioxidants) in Trichoderma and downregulated in Bacillus and vice versa in co-inoculation method (TB1). The metabolic changes in the co-culture promoted the maize plant growth and defense potential under normal and biotic stress conditions.ConclusionThe metabolites produced by the co-culture of T. asperellum and B. amyloliquefaciens improved the maize plant growth and defense potential under normal and biotic stress conditions.

show abstract

Optimization of a protective medium for enhancing the viability of freeze-dried Bacillus amyloliquefaciens B1408 based on response surface methodology

Cited by 27 publications

References 31 publications

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

Optimization of Production Parameters for Probiotic Lactobacillus Strains as Feed Additive

Bacillus based microbial formulations: Optimization of the production process

Simultaneous and sequential based co-fermentations of Trichoderma asperellum GDFS1009 and Bacillus amyloliquefaciens 1841: a strategy to enhance the gene expression and metabolites to improve the bio-control and plant growth promoting activity

Contact Info

Product

Resources

About