Optimization of biomass and astaxanthin production by the yeast Phaffia rhodozyma

Moriel, Danilo Gomes; Machado, Iara; Fontana, José D.; Bonfim, Tania Maria Bordin

doi:10.1590/s1516-93322004000300019

Cited by 3 publications

(8 citation statements)

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“…The cellular concentration of total carotenoids was determined using methods adapted from Hata and Hata (1971) and Moriel, Machado, Fontana, and Bonfim (2004). The fish were then packed in aluminium foil, which was labelled according to the fish number and aquarium and stored in a deep freezer (−80°C) until the biochemical determination analysis of carotenoids in the skin of goldfish juveniles.…”

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

“…Then, the 5 ml of acetone that was used to stiffen the integument was re-added and 1 ml of acetone was added to obtain the suggested volume described in Moriel et al (2004). The sample was centrifuged at 3,500 rpm for 5 min.…”

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

“…The methodology of carotenoid extraction used in the diets was the same as that used in the evaluation of the fish skin (Hata & Hata, 1971;Moriel et al, 2004), differentiating only the stage of centrifugation. The methodology of carotenoid extraction used in the diets was the same as that used in the evaluation of the fish skin (Hata & Hata, 1971;Moriel et al, 2004), differentiating only the stage of centrifugation.…”

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

“…Before the analysis, the fish samples were thawed for 2 hr at room temperature. The cellular concentration of total carotenoids was determined using methods adapted from Hata and Hata (1971) and Moriel, Machado, Fontana, and Bonfim (2004). The samples were immersed in a Falcon tube with 5 ml of acetone for 2 min with gentle manual shaking.…”

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

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Lutein as a natural carotenoid source: Effect on growth, survival and skin pigmentation of goldfish juveniles ( Carassius auratus )

et al. 2019

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This study aimed to evaluate the effect of lutein supplementation on growth, survival and skin pigmentation for goldfish juveniles. Four diets enriched with different carotenoid sources (lutein, astaxanthin, canthaxanthin and a combination of lutein and canthaxanthin) were compared to a control diet without carotenoid supplementation. The carotenoid inclusion level was standardized at 50 mg kg‐1 in all treatments. 240 goldfish juveniles (1.07�0.57 g) were cultivated in 30 aquariums (30L) during 84 days. The experimental design was completely randomized with five treatments and six replicates. The dietary inclusion of carotenoid pigments did not affect the growth and feeding efficiency of goldfish juveniles. Supplementation with lutein presented higher survival values when compared to the other treatments. Astaxanthin and canthaxanthin supplementation increased the concentration of carotenoids on the skin of goldfish juveniles in relation to the control treatment. For the fish fed with the diet containing lutein, the skin pigmentation was as efficient as astaxanthin and canthaxanthin, but did not differ from the control and combined treatment (canthaxanthin + lutein). The lutein supplementation (50 mg kg‐1) improved survival and promoted efficient carotenoid pigmentation on the skin of goldfish juveniles.

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Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

Section: Extraction and Quantification Of Carotenoidsmentioning

confidence: 99%

See 2 more Smart Citations

Lutein as a natural carotenoid source: Effect on growth, survival and skin pigmentation of goldfish juveniles ( Carassius auratus )

et al. 2019

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“…Os carotenoides são corantes naturais responsáveis pelas cores vermelha, amarela e laranja, muito empregadas nas indústrias alimentícias, farmacêutica, de cosméticos e até mesmo de ração animal (RODRIGUES-AMAYA, KIMURA, AMAYA-FARFAN, 2008).…”

Section: Introductionunclassified

BIOPRODUÇÃO DE CAROTENOIDES POR Xanthophyllomyces dendrorhous Y-10921 EM FRASCOS AGITADOS VARIANDO COMPOSIÇÃO DO MEIO DE CULTURA E CONDIÇÕES OPERACIONAIS

Urnau¹,

Tiggemann²,

Colet³

et al. 2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO-Os carotenoides são corantes naturais que podem ser sintetizados por plantas, algas e micro-organismos. Devido às suas propriedades corantes, ao seu potencial efeito benéfico na saúde humana e ao apelo pela substituição dos corantes sintéticos pelos naturais, os carotenoides têm recebido atenção especial, sendo que sua produção por micro-organismos pode tornar-se industrialmente praticável. Este estudo teve como objetivo analisar a influência do meio de cultura e as condições operacionais da bioprodução, na recuperação de carotenoides específicos produzidos por Xanthophyllmomyces dendrorhous Y-10921 em frascos agitados. A bioprodução foi realizada em frascos agitados em shaker, sem iluminação, variando-se a composição do meio YM (concentração de glicose, peptona, extrato de malte e de levedura), temperatura, agitação e pH inicial. A recuperação dos carotenoides foi realizada empregando dimetilsulfóxido (DMSO) para ruptura celular e extração acetona. A determinação dos carotenoides totais foi realizada espectrofotometricamente (λ= 474 nm). A máxima bioprodução de carotenoides (697 μg/g) foi observada no ensaio 1 onde o meio de cultura foi composto apenas por glicose (10 g/L), e maiores faixas de temperatura e pH (30ºC e 6, respectivamente). Palavras-chave: carotenoides, meio de cultura, Xanthophyllmomyces dendrorhous.

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Microbial Astaxanthin Production from Agro-Industrial Wastes—Raw Materials, Processes, and Quality

et al. 2022

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The antioxidant and food pigment astaxanthin (AX) can be produced by several microorganisms, in auto- or heterotrophic conditions. Regardless of the organism, AX concentrations in culture media are low, typically about 10–40 mg/L. Therefore, large amounts of nutrients and water are necessary to prepare culture media. Using low-cost substrates such as agro-industrial solid and liquid wastes is desirable for cost reduction. This opens up the opportunity of coupling AX production to other existing processes, taking advantage of available residues or co-products in a biorefinery approach. Indeed, the scientific literature shows that many attempts are being made to produce AX from residues. However, this brings challenges regarding raw material variability, process conditions, product titers, and downstream processing. This text overviews nutritional requirements and suitable culture media for producing AX-rich biomass: production and productivity ranges, residue pretreatment, and how the selected microorganism and culture media combinations affect further biomass production and quality. State-of-the-art technology indicates that, while H. pluvialis will remain an important source of AX, X. dendrorhous may be used in novel processes using residues.

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Optimization of biomass and astaxanthin production by the yeast Phaffia rhodozyma

Cited by 3 publications

References 9 publications

Lutein as a natural carotenoid source: Effect on growth, survival and skin pigmentation of goldfish juveniles ( Carassius auratus )

Lutein as a natural carotenoid source: Effect on growth, survival and skin pigmentation of goldfish juveniles ( Carassius auratus )

BIOPRODUÇÃO DE CAROTENOIDES POR Xanthophyllomyces dendrorhous Y-10921 EM FRASCOS AGITADOS VARIANDO COMPOSIÇÃO DO MEIO DE CULTURA E CONDIÇÕES OPERACIONAIS

Microbial Astaxanthin Production from Agro-Industrial Wastes—Raw Materials, Processes, and Quality

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