Optimization of ellagic acid production from ellagitannins by co-culture and correlation between its yield and activities of relevant enzymes

Huang, Wen; Niu, Hai; Li, Zhenshan; He, Yang; Gong, Weihong; Gong, Guohua

doi:10.1016/j.biortech.2007.01.032

Cited by 22 publications

(14 citation statements)

References 18 publications

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“…In case of Fig. 5 Huang et al [18] relationed the ET biodegradation with xylanase and cellulase activity and their responsibility in the EA accumulation, however in this work it was demonstrated that xylanase and cellulase activity is not correlated with ET degradation and EA accumulation. Xylanase activity was detected at 24-30 h of culture (42.97 U L À1 ) and cellulase activity was detected at 6-18 h of culture (31.96 U L À1 ), the EA accumulation was detected at 12-18 h in SSC, therefore, these enzymes are not directly related with the EA accumulation.…”

Section: Discussionmentioning

confidence: 57%

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Fungal biodegradation of pomegranate ellagitannins

Ascacio-Valdés

Buenrostro

Quiroz

et al. 2013

J. Basic Microbiol.

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Ellagitannins (ETs) are phytochemicals derived from secondary metabolism associated to defense system, with complex chemical structures, which have high participation during all stages of protection against microbial infection. In this study, we report the fungal biodegradation of a bioactive ET, named punicaline which was recovered and purified from pomegranate peels and used as carbon source in solid-state culture (SSC) using polyurethane as solid support. SSC was kinetically monitored during 36 h of incubation time. ETs and glycosides consumption were spectrophotometrically determined. Ellagic acid (EA) accumulation was analyzed by HPLC. Several enzymatic activities were assayed (cellulase, xylanase, β-glucosydase, polyphenoloxidase, tannase, and ET hydrolyzing activities). The consumption levels of ETs and glycosides were 66 and 40%, while EA accumulation reached 42.02 mg g(-1). A differential pattern of enzymatic activities was found; evidence from our studies suggests that the ET hydrolyzing activity is directly associated to EA accumulation, and production of this enzyme may represent the most critical step to successfully develop a bioprocess for production of an important bioactive compound, the EA.

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Section: Discussionmentioning

confidence: 57%

“…Xylanase activity [18] Xylanase activity was assayed using xylan as substrate. One milliliter reaction mixture, containing 0.5 ml of appropriately diluted enzyme solution and 0.5 ml of a 0.5% (w/v) suspension of xylan in 0.05 M citrate buffer, pH 5.4.…”

Section: Analytical Determinationsmentioning

confidence: 99%

Fungal biodegradation of pomegranate ellagitannins

Ascacio-Valdés

Buenrostro

Quiroz

et al. 2013

J. Basic Microbiol.

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“…However, in SSC, the information is limited to those studies reported by Shetty (2002, 2003), using cranberry pomace as support and source of ellagitannins with very low EA yields. Huang et al (2007c) suggested, for the first time, the presence of ellagitannin acyl hydrolase as the enzyme responsible of the EA accumulation, which indicates that a new tannase is involved in the biodegradation ellagitannins. Also, they reported that such enzyme had an synergistic activity with other enzymes as xylanase and cellulase to enhance the EA accumulation.…”

Section: Resultsmentioning

confidence: 97%

“…Recently, high EA yields (24%) were obtained after optimization of the co-culture of A. oryzae with Trichoderma reesei using acorn cups extract containing up to 62% ellagitannins as substrate (Huang et al 2007c). However, in SSC, the information is limited to those studies reported by Shetty (2002, 2003), using cranberry pomace as support and source of ellagitannins with very low EA yields.…”

Section: Resultsmentioning

confidence: 97%

Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture

Aguilera-Carbó

Hernández

Augur

et al. 2008

Food Bioprocess Technol

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The ability of Aspergillus niger GH1 in converting creosote bush ellagitannins into ellagic acid (EA) was evaluated in solid state culture. Creosote bush leaves were used to extract the ellagitannins fraction, which was impregnated in polyurethane foam used as support of solid state culture. Ellagitannins content, EA accumulation, and the related enzymatic activities were evaluated. A. niger GH1 was able to completely degrade creosote bush ellagitannins with an EA yield of 23.1% at 36 h of culture. The ability to degrade creosote bush ellagitannins exhibited by A. niger GH1 was clearly associated to an ellagitannin-hydrolysing enzyme with a maximum activity of 43 U/l, while that ability was not associated to tannase activity that was detected in the culture extract. This study demonstrated the great ability of A. niger GH1 to hydrolyze ellagitannins and the potential of solid state culture to produce the antioxidant EA by degradation of creosote bush ellagitannins.

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“…As soybean contains phenolic and isoflavonoid compounds concentrated mainly in seed matrix, it is possible that the fungi can play role in mobilization of polyphenolic compounds during SSF period (McCue et al ., 2003; McCue and Shetty, 2005, Huang et al ., 2008 Bhanja et al ., 2009). Isoflavonoids are polyphenolic compounds acting as reducing agents (free radical terminators), metal chelators and singlet oxygen quenchers (Mathew & Abraham 2006), which suggests the reduction and stabilization of AuNPs.…”

Section: Introductionmentioning

confidence: 99%

Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity

El-Batal¹,

Hashem

Abdelbaky³

2013

SpringerPlus

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Aspergillus oryzae was used to enhance the mobilization of antioxidants of soybean matrix along with garlic as a co-substrate by modulating polyphenolic substances during solid-state fermentation. Mobilized polyphenols were used as a green tool for synthesis and stabilization of gold nanoparticles (AuNPs). The radiation-induced AuNPs synthesis is a simple, clean and inexpensive process which involves radiolysis of aqueous solution that provides an efficient method to reduce metal ions. Gamma irradiated aqueous extract of fermented soybean and garlic was used for rapid preparation of AuNPs combining both effects of radiolytic reactions by radiation and stabilization by bioactive components of fermented extract. The synthesized AuNPs were confirmed by UV-Visible spectrophotometry, dynamic light scattering (DLS), Fourier Transform infra red (FT-IR) spectrophotometry, and transmission electron microscope (TEM) analysis which revealed morphology of spherical AuNPs with size ranging from 7–12 nm. The synthesized AuNPs exhibited antimicrobial activity against both Gram positive and Gram negative bacteria, as measured by well diffusion assay.

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Optimization of ellagic acid production from ellagitannins by co-culture and correlation between its yield and activities of relevant enzymes

Cited by 22 publications

References 18 publications

Fungal biodegradation of pomegranate ellagitannins

Fungal biodegradation of pomegranate ellagitannins

Ellagic Acid Production from Biodegradation of Creosote Bush Ellagitannins by Aspergillus niger in Solid State Culture

Gamma radiation mediated green synthesis of gold nanoparticles using fermented soybean-garlic aqueous extract and their antimicrobial activity

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