Optimization of Extracellular Acid Protease Production From Aspergillus Niger by Factorial Design

Kalaskar, Vishalkirti Vijay; Kasinathan, Narayanan; Subrahmanyam, V. M.; Rao, J. Venkata

doi:10.15414/jmbfs.2014.4.2.132-136

Cited by 7 publications

(7 citation statements)

References 13 publications

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“…AgNPs were synthesized under different physiochemical conditions to find optimum conditions favoring stable AgNPs of the smallest size range. The effect of incubation temperature on fungus growth and size of AgNPs was examined employing various temperature ranges, i.e., 25°C, 35°C, 45°C, and 55°C as reported previously [25]. The effect 2…”

Section: Optimization Of Conditions For Synthesis Of Silvermentioning

confidence: 99%

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Shahzad

Saeed

Iqtedar

et al. 2019

Journal of Nanomaterials

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The biogenesis of silver nanoparticles by fungi is an ecologically clean and nontoxic method compared to other physical and chemical methods. Thus, we aimed to discuss the mycosynthesis of extracellular size-controlled AgNPs. After comprehensive screening, Aspergillus fumigatus BTCB10 (KY486782) was selected for the synthesis of AgNPs of controlled size. Characterization was performed by UV-Vis spectrophotometer, Zetasizer, X-Ray Diffraction (XRD), FTIR (Fourier-transform infrared), Atomic Force Microscopy (AFM), and Scanning Electron Microscopy (SEM) along with functional assays—antibacterial and MTT assays. Data suggested that under optimized conditions, i.e., temperature 25°C, AgNO3 concentration 1 mM, biomass 7 g, fungal culture age 7 days, pH 6, ratio of cell-free filtrate (CFF)/silver nitrate (3 : 2), NaCl 20%, and under dark light, the smallest size AgNPs of 0.681 nm with 100% monodispersity was obtained as evident by a zeta potential of -23.4 mV, UV-Vis band at 400 nm, and the presence of O-H and C=O groups confirmed by ATR-FTIR; XRD revealed the crystalline nature of AgNPs; additionally, cube-shaped AgNPs were revealed by Scanning Electron Microscopy (SEM). Moreover, synthesized AgNPs exhibited antibacterial activity against multidrug-resistant bacterial strains, notably, Klebsiella pneumoniae BTCB04, Acinetobacter BTCB05, Pseudomonas aeruginosa BTCB01, and Escherichia coli BTCB03, while maximum 7-fold was observed with Acinetobacter BTCB05. AgNPs demonstrated no cytotoxic activity against HepG2 cells; however, in combination with cisplatin, antiproliferative and cytotoxic effects became more evident and significant in comparison to control and as single agent. Taken together, the data suggested that economical and smallest size AgNPs can be biosynthesized from Aspergillus fumigatus BTCB10 and be used as antibacterial and antiproliferative agents in combination with current drugs against clinically relevant multiple drug-resistant bacterial and tumoral cells. Further studies are required to confirm their effects employing in vivo disease models.

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Section: Optimization Of Conditions For Synthesis Of Silvermentioning

confidence: 99%

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Shahzad

Saeed

Iqtedar

et al. 2019

Journal of Nanomaterials

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“…The requirement for specific nitrogen supplements differs from organism to organism (Sharma et al 2017). In this study, peptone was chosen for use in the fermentation medium, because, among the nitrogen sources used for the composition of the media, this was considered the best source for the production of protease by Pseudomonas fluorescens (Kalaiarasi and Sunitha 2009), Aspergillus niger (Kalaskar et al 2014), Bacillus safensis (Shakir et al 2019). Species such as Rhizopus oryzae, Aspergillus niger, A. oryzae, A. terreus and Cellulomonas bogoriensis have shown optimal activity at 60 °C (Kumar et al 2005;Siala et al 2009;Castro and Sato 2014;Li et al 2016;Raol et al 2016), which was very close to the optimal temperature for M. speciosa proteolytic activity.…”

Section: Discussionmentioning

confidence: 99%

Characterization and immobilization of protease secreted by the fungus Moorella speciosa

et al. 2020

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Protease was extracellularly produced in submerged fermentation by the fungus Moorella speciosa with maximum activity of 8.6 × 10 3 U/mL. The optimal pH and temperature for enzyme activity were 6.78 and 60.88 °C, respectively. The enzyme was incubated in the presence of several ions at concentrations of 0.1 M and 0.01 M to address the effect on enzyme activity. Enzyme activity was increased by 56% and 130% in the presence of 0.1 M BaCl 2 and of 0.01 M Na 2 SO 4 , respectively. The V max and K m values were 0.01474 U/min/mg protein and 0.04190 mg/mL, respectively. The enzyme retained about 90% of enzymatic activity at 90 °C. Among the methods tested for enzyme immobilization, adsorption onto MAT540 carrier led to the most promising results, since after 15 reuse cycles up to 60% of the initial catalytic activity was retained. Entrapment in calcium alginate matrix allowed to retain up to 51% of the initial catalytic activity after 8 reuse cycles. This protease from M. speciosa, in either free or immobilized form, can be foreseen as a useful biocatalytic tool in process design by reducing operating costs, decreasing the use of chemical processing and, consequently, meeting the global demand for clean technologies.

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“…Free radical is one of the factors that induce the pathogenesis of a wide range of age-related degenerative diseases through the oxidative modification of DNA, proteins and vital molecules (Malathi and Chakraborty, 1991;Cohen, 1973). DPPH, a stable radical-generating reagent, was used in the present study for primary screening of the antiradical activities (Yokozawa et al, 1998;Kalaskar et al, 2014). The main antioxidant properties was mainly given by the peptides, which was hydrolyzed by enzyme and acid.…”

Section: Research Papermentioning

confidence: 99%

Antioxidant capacity of soybean protein isolate hydrolyzed with protease produced by Aspergillus 3.084 in vitro

Zong¹,

Jiang²,

Chen³

et al. 2019

Int. Biotechnol. Food Sci.

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Aspergillus, being known as a protease producing fungi, is used in Chinese and Korean traditional fermentation. In this paper, Aspergillus 3.084, one of the most common fungi in China fermentation industry, was employed to produce protease and hydrolyze soybean protein, and then the optimum extraction condition of protease was determined by the antioxidant activities of protease extraction solution. The protease was extracted from the culture medium, and performed in hydrolyzing soybean protein isolation. Extract condition of protease, hydrolysis ability of proteases of Aspergillus in hydrolyzing soybean protein isolation and the antioxidant ability of hydrolysate were investigated by detecting enzyme activity, degree of hydrolysis, scavenging activities of OH•, O2•and DPPH•. Maximum protease activity was obtained at 60 min, pH 7.5 and 32°C in extraction step. Optimum protease hydrolyzing time, E/S, temperature and pH for degree of hydrolysis and scavenging activities was 3 h, 6000, 37°C and 7.5, respectively.

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Optimization of Extracellular Acid Protease Production From Aspergillus Niger by Factorial Design

Cited by 7 publications

References 13 publications

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Size-Controlled Production of Silver Nanoparticles by Aspergillus fumigatus BTCB10: Likely Antibacterial and Cytotoxic Effects

Characterization and immobilization of protease secreted by the fungus Moorella speciosa

Antioxidant capacity of soybean protein isolate hydrolyzed with protease produced by Aspergillus 3.084 in vitro

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