Optimization of Factors for Enhanced Phycoremediation of Reactive Blue Azo Dye

Abstract

To cite this paper: Waqas, R., M. Arshad, H.N. Asghar and M. Asghar, 2015 AbstractSynthetic dyes are widely used in textile, leather and other dye-stuff industries. A large fraction of the dyes applied during the dyeing processes are released into wastewater. Therefore, the wastewater from dye-related industries is densely colored, with high chemical oxidation and biological oxidation demand. This wastewater must be treated prior to discharge into wastewater streams to prevent pollution of surface and groundwater, and minimizing risks to public health. The present study was undertaken for the first time in Pakistan to optimize the factors for algae possessing high potential to degrade Reactive Blue azo dye in liquid medium. Among eighty eight (88) isolates of alga, two showed high decolorization, which were used to optimize the decolorization of Reactive Blue azo dye. Both the selected algal strains showed an excellent potential to decolorize 100 mg L -1 Reactive Blue in seven days in the liquid broth.The optimum conditions determined for the two selected algal strains for decolorization of Reactive Blue (100 mg L -1 ) were pH 7.0, temperature 30°C, light conditions of 16 h, when inoculum size of 1 mL/10 mL was maintained and dye solution was supplemented with 5 g L -1 N and 5 g L -1 P as cosubstrates.

Plant microbe based remediation approaches in dye removal: A review

“…Algae decolorize the pigment in three distinct ways: To begin, algae collect algal biomass, CO 2 , and H 2 O via the use of chromophores; algae play an important role in the transition of chromophore elements to non-chromophore element; finally the resultant chromophores are absorbed on algae [ 149 ]. …”

“…Genetically modified E. coli SS125 were used for the breakdown of Remazol red dye by cloning the azoreductase gene from B. latrosporus RRK1 into E. coli DH5a and pAZR-SS125 [ 218 ]. Engineered E. coli JM109 (pGEX-AZR) strain in the laboratory that decolorizes direct blue 7[ 219 ]. Remazol red may be degraded in the presence of 0.8 mg/L of O 2 using the azoreductase gene from B. latrosporus RRK1 and inserted into E.coli [ 220 ].…”

Simultaneous bioremediation of Disperse orange-2RL Azo dye and fatty acids production by Scenedesmus obliquus cultured under mixotrophic and heterotrophic conditions

“…6) in the Disperse orange at pH 11 was obtained with mixotrophic (0.34 g), heterotrophic without glucose (0.43 g), heterotrophic with glucose was (0.48 g). In addition to having an effect on the cellular growth of algae, the pH had an effect on the enzyme activity that was responsible for the removal of the dye 44 . At high pH values, there was an increase in the amount of protein and biomass produced by S. obliquus 45 .…”

Decolorization and total nitrogen removal from batik effluent using alginate immobilized freshwater microalgae Chlorella sp.

“…to enhance the color removal efficiency. Moreover, a light-dark regime for efficient photosynthesis can encourage good color removal efficiency [25].…”