Optimization of Fibrin Glue Spray Systems for Ophthalmic Surgery

Chaurasia, Shyam S.; Champakalakshmi, Ravi; Angunawela, Romesh; Tan, Donald

doi:10.1167/tvst.1.2.2

Cited by 9 publications

(5 citation statements)

References 30 publications

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“…In the present report we demonstrated that unpassaged SVF cells isolated from human subjects by LipiVage, a minimally invasive clinically approved procedure that routinely provides a high yield of viable adipocytes (11), and processed in a closed system (Tissue Genesis Cell Isolation System) could be suspended in Tisseel fibrin sealant and delivered using a commercially available aerosol spray system (12) while retaining the ability to differentiate into vessel-like endothelial tubules and adipocytes.…”

Section: Discussionmentioning

confidence: 93%

Human adipose stromal vascular cell delivery in a fibrin spray

Zimmerlin¹,

Rubin²,

Pfeifer³

et al. 2013

Cytotherapy

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Background Adipose tissue represents a practical source of autologous mesenchymal stromal cells (MSC) and vascular-endothelial progenitor cells, available for regenerative therapy without in vitro expansion. One of the problems confronting the therapeutic application of such cells is how to immobilize them at the wound site. Here, we evaluated in vitro the growth and differentiation of human adipose stromal vascular fraction (SVF) cells after delivery using a fibrin spray system. Methods SVF cells were harvested from four human adult patients undergoing elective abdominoplasty using the LipiVage™ system. After collagenase digestion, mesenchymal and endothelial progenitor cells (pericytes, supra-adventitial stromal cells, endothelial progenitors) were quantified by flow cytometry before culture. SVF cells were applied to culture vessels using the Tisseel™ fibrin spray system. SVF cell growth and differentiation was documented by immunofluorescence staining and photomicrography. Results SVF cells remained viable following application and were expanded up to three weeks, when they reached confluence and adipogenic differentiation. Under angiogenic conditions, SVF cells formed endothelial (vWF+, CD31+ and CD34+) tubules surrounded by CD146+ and α-SMA+ perivascular/stromal cells. Discussion Human adipose tissue is a rich source of autologous stem cells, which are readily available for regenerative applications such as wound healing, without in vitro expansion. Our results indicate that mesenchymal and endothelial progenitor cells, prepared in a closed system from unpassaged lipoaspirate samples, retain their growth and differentiation capacity when applied and immobilized on a substrate using a clinically approved fibrin sealant spray system.

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Section: Discussionmentioning

confidence: 93%

Human adipose stromal vascular cell delivery in a fibrin spray

Zimmerlin¹,

Rubin²,

Pfeifer³

et al. 2013

Cytotherapy

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“…Indeed, the time needed for the FG to crosslink is strongly affected by the thrombin/fibrinogen concentration, which determines the setting time of the fibrin sealant, ranging from 30 s (rapid setting) to several minutes (slow setting). [ 28 ] The composition also affects the conversion of fibrinogen into fibrin clots, which form a mesh and an dense protofibril packing with a nanometer size, which probably impedes the entanglement of the GG polymeric chains (the average weight of the polymeric chains is 500 000 Da) upon FG assembly. This may hamper material penetration within the crosslinked solution over time.…”

Section: Resultsmentioning

confidence: 99%

Primers for the Adhesion of Gellan Gum‐Based Hydrogels to the Cartilage: A Comparative Study

Trucco

Riacci

Vannozzi

et al. 2022

Macromolecular Bioscience

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A stable adhesion to the cartilage is a crucial requisite for hydrogels used for cartilage regeneration. Indeed, a weak interface between the tissue and the implanted material may produce a premature detachment and thus the failure of the regeneration processes. Fibrin glue, cellulose nanofibers and catecholamines have been proposed in the state-of-the-art as primers to improve the adhesion. However, no studies focused on a systematic comparison of their performance. This work aims to evaluate the adhesion strength between ex vivo cartilage specimens and polysaccharide hydrogels (gellan gum and methacrylated gellan gum), by applying the mentioned primers as intermediate layer. Results show that the fibrin glue and the cellulose nanofibers improve the adhesion strength, while catecholamines do not guarantee reaching a clinically acceptable value. Stem cells embedded in gellan gum hydrogels reduce the adhesion strength when fibrin glue is used as a primer, being anyhow still sufficient for in vivo applications.

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“…In conclusion, careful attention and precisely controlled placement are required during blow-out fracture surgery using fibrin glue. Even if the surgical field of view is narrow, we must visually check whether fibrin glue is applied properly to the desired area with a homogeneous thickness, and a spray applicator can help for this purpose [ 16 ]. After application, tissue traction should be maintained until clot formation.…”

Section: Discussionmentioning

confidence: 99%

Limited eye movement caused by clumping of fibrin glue used in blowout fracture surgery: a care report

Shin

Lee

Kim

et al. 2022

Arch Craniofac Surg

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Fibrin glue is a topical agent widely used for hemostasis, wound healing, and surgical adhesion. Complications of fibrin glue itself are extremely rare because it is absorbed over time, but can occur as a result of inappropriate application. We report a case of a postoperative complication caused by inappropriate application of fibrin glue in blow-out fracture surgery. A 65-year-old male patient presented with periorbital swelling and an open wound on the right infraorbital area. Computed tomography showed a right orbital floor fracture. After reduction of the herniated tissue into the orbit, an implant was inserted and fibrin glue was applied to stabilize the implant. This procedure was performed without difficulty, but the patient complained of persistent diplopia and limited eyeball movement after surgery. An imaging study showed a mass-like lesion, which was not a hematoma, in the orbital cavity. In a second operation, the mass was identified as clotted fibrin glue that had not been applied properly. After removal, the patient’s symptoms were relieved without further complications. Appropriate and careful application of fibrin glue is necessary to avoid unnecessary complications.

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Optimization of Fibrin Glue Spray Systems for Ophthalmic Surgery

Abstract: The standardization of the spray system for the application of FG in ophthalmology will provide an economical method for delivering consistent healing results after surgery.

Cited by 9 publications

References 30 publications

Human adipose stromal vascular cell delivery in a fibrin spray

Human adipose stromal vascular cell delivery in a fibrin spray

Primers for the Adhesion of Gellan Gum‐Based Hydrogels to the Cartilage: A Comparative Study

Limited eye movement caused by clumping of fibrin glue used in blowout fracture surgery: a care report

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