Primers for the Adhesion of Gellan Gum‐Based Hydrogels to the Cartilage: A Comparative Study

Trucco, Diego; Riacci, Laura; Vannozzi, Lorenzo; Manferdini, Cristina; Arrico, Lorenzo; Gabusi, Elena; Lisignoli, Gina; Ricotti, Leonardo

doi:10.1002/mabi.202200096

Cited by 15 publications

(9 citation statements)

References 33 publications

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“…Gellan gum is an anionic polymer that interacts with monovalent (Na + , K + ) and divalent (Ca 2+ ) cations in tears to form hyaline gels and has good in situ gelation properties at polymer concentrations as low as 0.1% (w/v). Deacetylated gellan gum (DGG) is created by polymerizing tetrasaccharide units, and its gelling ability is enhanced by weakening the molecular space barrier via deacetylation 86 . DGG is an extracellular polysaccharide produced by Pseudomonas aeruginosa , a Gram‐negative bacterium isolated from water lilies, which has a parallel double helix structure, with the entire gel chain consisting of four base units for repetitive curing 87 .…”

Section: Classification and Characteristics Of In Situ Gelsmentioning

confidence: 99%

“…Deacetylated gellan gum (DGG) is created by polymerizing tetrasaccharide units, and its gelling ability is enhanced by weakening the molecular space barrier via deacetylation. 86 DGG is an extracellular polysaccharide produced by Pseudomonas aeruginosa, a Gram-negative bacterium isolated from water lilies, which has a parallel double helix structure, with the entire gel chain consisting of four base units for repetitive curing. 87 As a result, at a certain solution concentration, it exhibits cation-induced gelling.…”

Section: Gellan Gluementioning

confidence: 99%

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In situ gels: The next new frontier in ophthalmic drug delivery system

Sha

Liu

Jiang

et al. 2023

Polymers for Advanced Techs

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Recently, the eye drug delivery system has received increasing attention. The in situ ophthalmic gel is a semisolid ophthalmic preparation that can be changed in the eyes immediately after the solution is administered, showing unique advantages as a new drug delivery system. Although there are still some problems to be solved, the in situ ophthalmic gel is a promising drug delivery system to treat ocular diseases, due to its properties in improving the bioavailability, prolonging the retention time of the drug, producing a sustained-release effect, and possessing little toxicity and irritation. In this review, the characteristics, classification, ocular barrier, and route of administration of in situ ophthalmic gel have been introduced in detail for expanding the horizon of nanoscale technologies in the treatment of ocular diseases in the foreseeable future.

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Section: Classification and Characteristics Of In Situ Gelsmentioning

confidence: 99%

Section: Gellan Gluementioning

confidence: 99%

In situ gels: The next new frontier in ophthalmic drug delivery system

Sha

Liu

Jiang

et al. 2023

Polymers for Advanced Techs

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“…The primer conduct was filled with a Pluronic-CNFs solution (20% w/v Pluronic F127, 0.5% w/v CNFs) to improve the adhesion strength of the printed construct to the surrounding tissue, as suggested by the state-of-the-art. 28 , 49 CNFs were added to the Pluronic solution in an ice bath while stirring for 1 h. Once dissolved, the solution was kept in the refrigerator at + 4 °C until use A black tissue marking dye (Trajan, Davidson Marking System) was added to the SA-Pluronic solution to visually discriminate it from the shell and primer formulations for the material extrusion verification.…”

Section: Preparation Of Materials and Rheometric Analysesmentioning

confidence: 99%

A Novel Approach for Multiple Material Extrusion in Arthroscopic Knee Surgery

et al. 2022

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Articular cartilage defects and degenerative diseases are pathological conditions that cause pain and the progressive loss of joint functionalities. The most severe cases are treated through partial or complete joint replacement with prostheses, even if the interest in cartilage regeneration and re-growth methods is steadily increasing. These methods consist of the targeted deposition of biomaterials. Only a few tools have been developed so far for performing these procedures in a minimally invasive way. This work presents an innovative device for the direct deposition of multiple biomaterials in an arthroscopic scenario. The tool is easily handleable and allows the extrusion of three different materials simultaneously. It is also equipped with a flexible tip to reach remote areas of the damaged cartilage. Three channels are arranged coaxially and a spring-based dip-coating approach allows the fabrication and assembly of a bendable polymeric tip. Experimental tests were performed to characterize the tip, showing the ability to bend it up to 90° (using a force of ~ 1.5 N) and to extrude three coaxial biomaterials at the same time with both tip straight and tip fully bent. Rheometric analysis and fluid-dynamic computational simulations were performed to analyze the fluids’ behavior; the maximum shear stresses were observed in correspondence to the distal tip and the channel convergence chamber, but with values up to ~ 1.2 kPa, compatible with a safe extrusion of biomaterials, even laden with cells. The cells viability was assessed after the extrusion with Live/Dead assay, confirming the safety of the extrusion procedures. Finally, the tool was tested arthroscopically in a cadaveric knee, demonstrating its ability to deliver the biomaterial in different areas, even ones that are typically hard-to-reach with traditional tools.

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“…Hydrogels can be excellent hosts for MSCs, and the therapeutic advantage of this strategy is to protect the cells injected into the defect (i.e., from shear forces and needle dimensions) and at the same time to favor their adhesion to the cartilage [ 20 , 25 , 26 , 34 ]. In fact, the hydrogels provide mechanical support, elasticity and stiffness and facilitate cell interactions with OA cartilage [ 5 , 25 , 26 , 35 ].…”

Section: Introductionmentioning

confidence: 99%

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

Manferdini

Gabusi

Saleh

et al. 2022

Cells

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This systematic review is focused on the main characteristics of the hydrogels used for embedding the mesenchymal stromal cells (MSCs) in in vitro/ex vivo studies, in vivo OA models and clinical trials for favoring cartilage regeneration in osteoarthritis (OA). PubMED and Embase databases were used to select the papers that were submitted to a public reference manager Rayyan Systematic Review Screening Software. A total of 42 studies were considered eligible: 25 articles concerned in vitro studies, 2 in vitro and ex vivo ones, 5 in vitro and in vivo ones, 8 in vivo ones and 2 clinical trials. Some in vitro studies evidenced a rheological characterization of the hydrogels and description of the crosslinking methods. Only 37.5% of the studies considered at the same time chondrogenic, fibrotic and hypertrophic markers. Ex vivo studies focused on hydrogel adhesion properties and the modification of MSC-laden hydrogels subjected to compression tests. In vivo studies evidenced the effect of cell-laden hydrogels in OA animal models or defined the chondrogenic potentiality of the cells in subcutaneous implantation models. Clinical studies confirmed the positive impact of these treatments on patients with OA. To speed the translation to the clinical use of cell-laden hydrogels, further studies on hydrogel characteristics, injection modalities, chemo-attractant properties and adhesion strength are needed.

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Primers for the Adhesion of Gellan Gum‐Based Hydrogels to the Cartilage: A Comparative Study

Cited by 15 publications

References 33 publications

In situ gels: The next new frontier in ophthalmic drug delivery system

In situ gels: The next new frontier in ophthalmic drug delivery system

A Novel Approach for Multiple Material Extrusion in Arthroscopic Knee Surgery

Mesenchymal Stromal Cells Laden in Hydrogels for Osteoarthritis Cartilage Regeneration: A Systematic Review from In Vitro Studies to Clinical Applications

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