Optimization of growth and production of protease by Penicillium species using submerged fermentation

K, Vamsi Krishna; Gupta, Mona; Gupta, Nikhil; Gaudani, Hipal; Trivedi, Soham; Patil, Prasad; Gupta, Girish Kumar; Khairnar, Yogesh; Borasate, Amol; Mishra, Dharmendra Kumar; Mumbai, Navi

doi:10.9735/0975-5276.1.1.14-18

Cited by 10 publications

(5 citation statements)

References 5 publications

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“…Protease is widely distributed among microorganisms including fungi, bacteria, and actinomycetes (Ma 2013). Among fungi genera, Aspergillus, Penicillium, Paecilomyces, Rhizopus and Rhizomucor are well-known producers of proteases (Devi 2008, Sindhu et al 2009, Krishna 2009). Patil et al (2015) reported that nine different fungi concluding Cladosporium sp., Rhizoctonia sp., Aspergillus sp., Chaetomium sp., Biosporus sp., Fusarium sp., Curvularia sp., Cladosporium sp., and Colletotrichum sp.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Enzyme Protease Activity and Inhibition Effect on Pyricularia grisea with the Leaf Extract of Commela communis L.

Truc¹,

Ha²,

Trâm³

et al. 2019

J Pure Appl Microbiol

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Rice blast is a dangerous disease that causes major damage to rice productivity worldwide. The purpose of this report is to examine the protease activity of Pyricularia grisea and the effects of Commelina communis L. (common name "day flower") extract on mycelial growth. The result showed that three isolates were collected from wild rice (Oryza rufipogon) based on a designation of new international standard and namely as U43-i4-k024-z05-ta532 (isolate 1); U12-i0-k101-z05-ta102 (isolate 2) and U43-i4-k141-z15-ta522 (isolate 3). Particularly, the third isolate showed the highest protease enzyme activity of 30.11 U/ml, followed by isolate 1 (20.27 U/ml) and isolate 2 (12.81 U/ml). The new fungi isolated from wild rice may be important methods to control detrimental diseases infesting cultivated rice. The result showed methanol extract from the day flower inhibitions on P.grisea. Besides protease activity, fungi inhibition was also affected. The protease activity was low; the mycelial growth inhibition was high (IC 50 = 2.35mg/mL at Isolate 2). Plant extracts from the day flower saved money and were environmentally friendly. So the implementation of this substance is very essential.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Enzyme Protease Activity and Inhibition Effect on Pyricularia grisea with the Leaf Extract of Commela communis L.

Truc¹,

Ha²,

Trâm³

et al. 2019

J Pure Appl Microbiol

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“…S. fimicola S2 and S. fimicola N6 out of the total six strains presented largest zone around the colony of fungus, so they were selected for next work. Aspergillus, Penicillium, Rhizopus, and Rhizomucor produce proteases in large quantities (Krishna et al, 2009;Sindhu et al, 2009) and are efficient over a wide range of pH (Rao et al, 1998).…”

Section: /13mentioning

confidence: 99%

Protease production and molecular characterization of a protease dipeptidyl-aminopeptidase gene from different strains of Sordaria fimicola

et al. 2024

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The current research was designed to reach extracellular protease production potential in different strains of Sordaria fimicola which were previously obtained from Dr. Lamb (Imperial College, London) from North Facing Slope and South Facing Slope of Evolution Canyon. After initial and secondary screening, two hyper-producers strains S2 and N6 were selected for submerged fermentation and cultural conditions including temperature, pH, incubation period, inoculum size, substrate concentration, and different carbon and nitrogen sources were optimized for enzyme production. S2 strain showed maximum protease production of 3.291 U/mL after 14 days of incubation at 30 °C with 7 pH, 1% substrate concentration and 1 mL inoculum, While N6 strain showed maximum protease production of 1.929 U/mL under fermentation optimized conditions. Another aim of the present research was to underpin the biodiversity of genetics and post-translational modifications (PTMs) of protease DPAP (peptidyl-aminopeptidase) in Sordaria fimicola. Five polymorphic sites were observed in amino acid sequence of S. fimicola strains with reference to Neurospora crassa. PTMs prediction from bioinformatics tools predicted 38 phosphorylation sites on serine residues for protease peptidyl-aminopeptidase in S1 strain of S. fimicola while 45 phosphorylation sites on serine in N7 strain and 47 serine phosphorylation modifications were predicted in N. crassa. Current research gave an insight that change in genetic makeup effected PTMs which ultimately affected the production of protease enzyme in different strains of same organism (S. fimicola). The production and molecular data of the research revealed that environmental stress has strong effects on the specific genes through mutations which may cause genetic diversity. S. fimicola is non- pathogenic fungus and has a short life cycle. This fungus can be chosen to produce protease enzyme on a commercial scale.

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“…A máxima atividade das enzimas foi observada a 25ºC, 50°C e 60°C para os isolados do gênero Aspergillus, sugerindo a presença de grupos distintos de proteases, a exemplo de Aspergillus N44. Para Penicillium N23, a temperatura ótima das proteases foi obtida a 40°C, característica diferenciada dos resultados publicados para Penicillium spp., cuja temperatura ótima de atividade proteolítica costuma ser descrita na faixa de 27°C e 30°C (KRISHNA et al, 2009 Com relação ao pH, a atividade das proteases de maiores valores foi determinada na faixa de 6,0 a 9,0. Aspergillus N34 foi o único isolado cujo pH ótimo foi levemente ácido (pH=6,0), resultado semelhante ao citado por Thichota et al (2010).…”

Section: Atividade De Proteasesunclassified

Enzimas Extracelulares de Fungos Anamórficos Isolados de Morinda citrifolia L.

Tavares¹,

Fonseca²,

Fonseca³

et al. 2013

BBR

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The Amazon region is home to a vast microbial diversity that is attracting great interest in the industrial sector, like the anamorphic fungi that has economic value because of the production of bioactive compounds for industrial application. The aim of this study was to isolate and identify fungi from healthy leaf structure of Morinda citrifolia and verify the production of enzymes, as well as characterizing proteases from Aspergillus and Penicillium for industrial application. For the determination of enzyme activities, the fungi were grown in liquid medium (extract MGYP) at 30°C and 150 rpm. After five days, the crude extract was recovered by membrane filtration (22μm) and activity was determined by the cup plate, at 37°C for 18 hours on solid medium with substrate for each enzyme. For visualization of the areas of substrate degradation on the surface of the medium were added solution of Congo Red and 1M NaCl solution to detect cellulase and iodine vapors for detecting amylase. All statements expressed enzyme (amylase, cellulase and protease), but increased expression of the halo was Aspergillus N42 and N34 (cellulases). The protease activity of Aspergillus ranged from 1.36 U/mL (N44) at 3.38 U/ml (N35), whilst for Penicillium N23 values were higher (10.82 U/mL). As to pH, the best results were obtained in a range of 6-9. All isolates tested can be sources of enzymes for future applications in industrial processes. rEsumoA região Amazônica abriga uma vasta diversidade microbiana que vem despertando grande interesse no setor industrial, a exemplo dos fungos anamórficos, que tem valor econômico em virtude da produção de compostos bioativos para aplicação industrial. O objetivo desse estudo foi isolar e identificar fungos da estrutura foliar sadia de Morinda citrifolia e verificar a produção de enzimas, assim como caracterizar proteases de Aspergillus e Penicillium para aplicação industrial. Para a determinação das atividades enzimáticas, os fungos foram cultivados em meio líquido (extrato MGYP), a 30°C e 150 rpm. Após cinco dias, o extrato bruto foi recuperado por filtração em membrana (22µm) e a atividade foi determinada pelo método cup plate, a 37°C por 18 horas em meio sólido adicionado de substrato indutor para cada enzima. Para visualização das áreas de degradação do substrato, na superfície do meio foram adicionadas solução de Vermelho Congo e, solução de NaCl 1M para detectar celulase e vapores de iodo para detectar amilase. Todos os extratos expressaram atividade enzimática (amilases, celulases e proteases), porém o halo de maior diâmetro foi de Aspergillus N42 e N34 (celulases). A atividade das proteases das linhagens de Aspergillus variou de 1,36 U/mL (N44) a 3,38 U/mL (N35), enquanto para Penicillium N23 os valores foram superiores (10,82 U/mL). Quanto ao pH, os melhores resultados foram obtidos numa faixa de 6-9. Todos os isolados testados podem ser fontes de enzimas para futuras aplicações em processos industriais.Palavras-chave: deuteromicetos, bioprocessos, enzimas hidrolíticas

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Optimization of growth and production of protease by Penicillium species using submerged fermentation

Cited by 10 publications

References 5 publications

Evaluation of Enzyme Protease Activity and Inhibition Effect on Pyricularia grisea with the Leaf Extract of Commela communis L.

Evaluation of Enzyme Protease Activity and Inhibition Effect on Pyricularia grisea with the Leaf Extract of Commela communis L.

Protease production and molecular characterization of a protease dipeptidyl-aminopeptidase gene from different strains of Sordaria fimicola

Enzimas Extracelulares de Fungos Anamórficos Isolados de Morinda citrifolia L.

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