Optimization of growth requirements and scale‐up cultivation of freshwater algae<i>Desmodesmus armatus</i>using response surface methodology

Wang, Shenglin; Cao, Meng; Wang, Bowen; Deng, Ruru; Gao, Yumei; Liu, Pinghuai

doi:10.1111/are.14290

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…The RSM is an interesting approach for the optimization of biomass and specific metabolites. Over the years, this method has been proven to be an interesting statistical tool for better culture media in strains such as Ankistrodesmus falcatus KJ671624, 48 Auxenochlorella protothecoides , 49 Botryococcus braunii , 24 B. braunii LB572, 50 Chlorella sp., 51 Chlorellaprotothecoides , 52 Chlorella sorokiniana Mb-1, 53 Chlorella vulgaris , 54 C. vulgaris 1803, 55 Chlorococcum oleofaciens , 17 Desmodesmus armtus , 56 Dunaliella salina , 57 C. pyrenoidosa , 58 Scenedesmus sp. ASK22, 59 Scenedesmus obliquus , 60 , 61 and Spirulina sp.…”

Section: Discussionmentioning

confidence: 99%

“…braunii LB572, Chlorella sp., Chlorellaprotothecoides, Chlorella sorokiniana Mb-1, Chlorella vulgaris, C. vulgaris 1803, Chlorococcum oleofaciens, Desmodesmus armtus, Dunaliella salina, C. pyrenoidosa, Scenedesmus sp.…”

Section: Discussionmentioning

confidence: 99%

“…ASK22, Scenedesmus obliquus, , and Spirulina sp . In PBP research, the application of RSM has been focused on their extraction and purification. − Until now, there are few reports such as the works of Singh et al . and Mogany et al.…”

Section: Discussionmentioning

confidence: 99%

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Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

et al. 2021

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Phycobiliproteins (PBPs) are a group of brilliant pigment proteins found in cyanobacteria and red algae; their synthesis and accumulation depend on several factors such as the type of strain employed, nutrient concentration, light intensity, light regimes, and others. This study evaluates the effect of macronutrients (citrate buffer, NaNO 3 , K 2 HPO 4 , MgSO 4 , CaCl 2 , Na 2 CO 3 , and EDTA) and the concentration of trace metals in BG-11 media on the accumulation of PBPs in a thermotolerant strain of Oscillatoria sp. The strain was grown in BG-11 media at 28 °C with a light:dark cycle of 12:12 h at 100 μmol m –2 s –1 for 15 days, and the effect of nutrients was evaluated using a Plackett–Burman Design followed by optimization using a response surface methodology. Results from the concentration of trace metals show that it can be reduced up to half-strength in its initial concentration without affecting both biomass and PBPs. Results from the Plackett–Burman Design revealed that only NaNO 3 , Na 2 CO 3 , and K 2 HPO 4 show a significant increase in PBP production. Optimization employed a central Non-Factorial Response Surface Design with three levels and four factors (3 4 ) using NaNO 3 , Na 2 CO 3 , K 2 HPO 4 , and trace metals as variables, while the other components of BG-11 media (citrate buffer, MgSO 4 , CaCl 2 , and EDTA) were used in half of their initial concentration. Results from the optimization show that interaction between Na 2 CO 3 and K 2 HPO 4 highly increased PBPs’ concentration, with values of 15.21, 3.95, and 1.89 (% w/w), respectively. These results demonstrate that identifying and adjusting the concentration of critical nutrients can increase the concentration of PBPs up to two times for phycocyanin and allophycocyanin while four times for phycoerythrin. Finally, the reduction in non-key nutrients’ concentration will reduce the production costs of colorants at an industrial scale and increase the sustainability of the process.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

et al. 2021

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“…5, increasing light intensity appeared to be effective for carotenoid yield. Wang et al 2019 reported that optimized medium composition by Central Composite Design (CCD) for freshwater alga Desmodesmus armatus was stated indicated the nitrate concentration of 0.93 g L -1 under the light intensity of 108 μmol/m 2 s, at 27 °C, at pH 7.00 and air flow rate of 0.50 L/min.…”

Section: Determination Of Carotenoid Concentration and Statistical Analysismentioning

confidence: 99%

Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Şener¹,

Demirel²,

İmamoğlu³

et al. 2021

ase

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Commercial carotenoids of green microalgae have become significant especially for their applications in the cosmetic, pharmaceutical, food and feed industries. Effects of physical and chemical parameters on carotenoid contents in isolated microalgal species have been investigated. The variables of shaking rate, nitrogen concentration and light intensity affect biomass production and the synthesis of carotenoids in the green microalgae were investigated using the statistical design by Box-Behnken (BBD) employing Response Surface Methodology (RSM). Furthermore, the optimized cultivation conditions using BBD for Chlorella vulgaris, Ankistrodesmus convolutus, Dunaliella salina, Tetraselmis striata were determined using the spectrophotometric method to enhance carotenoid concentration. A. convolutus within the green algae was detected with the highest carotenoid concentration. The optimum conditions results indicated that the growth of A. convolutus (0.55 mg/L) and production of total carotenoids (25.1138 mg/g biomass) were found at the stirrer rate of 100 rpm under the light intensity of 100 μE/m 2 s, and in the nutrient component of 8.82 mM NaNO 3 . These conditions were validated experimentally for total carotenoid yield (24.13 mg/g biomass). After that the production was performed in a flat-plate photobioreactor with a volume of 6L based on the optimized conditions and the carotenoid profile was defined by HPLC-DAD using standards such as violaxanthin, astaxanthin and β-carotene. This study proposes that the RSM approach can be used to define optimal conditions for large-scale production of carotenoids by A. convolutus.

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“…One available tool is the application of the design of experiments (DoE) [88][89][90][91][92] to improve concentrations of specific metabolites. This type of experiment has been successfully applied to improve the deposition of specific metabolites in algae and cyanobacteria before [93][94][95][96][97][98], especially in the selection of the carbon and nitrogen sources and their specific concentrations. However, to the best of our knowledge, there is no scientific literature on the optimization of culture media for strains from high-mountain environments.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Metabolite Production in High-Altitude Microalgal Strains by Optimized C/N/P Ratio

et al. 2022

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This study evaluated the role of C/N/P in the increase in the synthesis of carbohydrates, proteins, and lipids in two high-mountain strains of algae (Chlorella sp. UFPS019 and Desmodesmus sp. UFPS021). Three carbon sources (sodium acetate, sodium carbonate, and sodium bicarbonate), and the sources of nitrogen (NaNO3) and phosphate (KH2PO4 and K2HPO4) were analyzed using a surface response (3 factors, 2 levels). In Chlorella sp. UFPS019, the optimal conditions to enhance the synthesis of carbohydrates were high sodium carbonate content (3.53 g/L), high KH2PO4 and K2HPO4 content (0.06 and 0.14 g/L, respectively), and medium-high NaNO3 (0.1875 g/L). In the case of lipids, a high concentration of sodium acetate (1.19 g/L) coupled with high KH2PO4 and K2HPO4 content (0.056 and 0.131 g/L, respectively) and a low concentration of NaNO3 (0.075 g/L) drastically induced the synthesis of lipids. In the case of Desmodesmus sp. UFPS021, the protein content was increased using high sodium acetate (2 g/L), high KH2PO4 and K2HPO4 content (0.056 and 0.131 g/L, respectively), and high NaNO3 concentration (0.25 g/L). These results demonstrate that the correct adjustment of the C/N/P ratio can enhance the capacity of high-mountain strains of algae to produce high concentrations of carbohydrates, proteins, and lipids.

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Optimization of growth requirements and scale‐up cultivation of freshwater algaeDesmodesmus armatususing response surface methodology

Cited by 8 publications

References 33 publications

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

Enhancement of Phycobiliprotein Accumulation in Thermotolerant Oscillatoria sp. through Media Optimization

Optimization of Culture Conditions for Total Carotenoid Amount Using Response Surface Methodology in Green Microalgae / Ankistrodesmus convolutus

Enhancement of Metabolite Production in High-Altitude Microalgal Strains by Optimized C/N/P Ratio

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