Optimization of growth requirements of marine diatomChaetoceros muelleriusing Response Surface Methodology

Abstract

The marine centric diatom Chaetoceros muelleri has been widely used as live feed in fish and shellfish aquaculture due to its excellent nutritional properties. The growth of microalgae is affected by various nutritional and environmental parameters, and species specific optimization of these parameters is essential for the development of cost-effective biomass production process. In this study, the growth of C. muelleri, was optimized using response surface methodology (RSM). The variables nitrate, phosphate, silicate, temperature, pH, salinity and agitation speed were initially screened for biomass production in C. muelleri using Plackett-Burman experimental design, and it was found that nitrate, phosphate, silicate, temperature and pH significantly influenced the biomass production. These variables were further optimized by central composite design of RSM for biomass production and nutrient composition, and the medium was re-constituted accordingly to have 180 mg L À1 nitrate, 7.5 mg L À1 phosphate, 30 mg L À1 silicate, with optimum growth conditions of temperature at 31°C and pH 6.46. At the end of 10 days culture period under the above conditions, biomass, protein, lipid and carbohydrate significantly increased from 0.360 AE 0.01 mg L À1 , 9.41 AE 0.02%, 18.11 AE 0.01%, 0.6 AE 0.02% to 1.16 AE 0.01 mg L À1 , 11.02 AE 0.01%, 19.58 AE 0.01% and 0.77 AE 0.01% respectively. The biomass production of C. muelleri could be increased 3.2-fold with an improved nutrient profile by modifying the growth factors, the study thus offering an optimized process for biomass production of C. muelleri.

Enhancement of Biomass Production of Diatom Nitzschia sp. S5 through Optimisation of Growth Medium Composition and Fed-Batch Cultivation

“…The highest biomass concentration and biomass productivity (0.73 g L −1 and 52.04 mg L −1 day −1 , respectively) were obtained at a N:Si:P ratio of 7:23:1. This value differs from Redfield's ratio (Si:N:P = 15:16:1 mol:mol:mol), generally accepted for diatoms, but deviations from this ratio are reported in the literature for the same diatom strains, especially when culture media composition or growth conditions significantly vary [20,76,77]. Usually, it is used as an indicator of nutrient limitation (Si:N:P molar ratio below 15:16:1 mol:mol:mol) [78].…”

Trends in Diatom Research Since 1991 Based on Topic Modeling

“…Most primary and secondary consumers in aquatic ecosystems depend on diatoms to some extent and even exclusively [6,7]. There are also many fish, shrimp and shellfish species of great fishery values that mainly feed on diatoms [8,9,10]. Therefore, diatoms are the main carbon source fueling a wide range of aquatic food webs and supporting a large proportion of global fisheries [11,12].…”

Optimization of growth requirements and scale‐up cultivation of freshwater algaeDesmodesmus armatususing response surface methodology

“…However, the single‐factor experiment to optimized cultivation conditions of microalgae could not reflect the interaction between different factors. Therefore, the response surface method (RSM) has been widely used (Hallenbeck, Grogger, Mraz, & Veverka, ; Kumaran, Jose, Joseph, & Singh, ; Tongprawhan, Srinuanpan, & Cheirsilp, ). Although Desmodesmus armatus has not been applied to aquaculture, it has high nutritional value and the optimization of its biomass and biomass production process has not been reported.…”

“…However, the single-factor experiment to optimized cultivation conditions of microalgae could not reflect the interaction between different factors. Therefore, the response surface method (RSM) has been widely used (Hallenbeck, Grogger, Mraz, & Veverka, 2015;Kumaran, Jose, Joseph, & Singh, 2017;Tongprawhan, Srinuanpan, & Cheirsilp, 2014).…”