2015
DOI: 10.1590/s0100-204x2015001200015
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Optimization of heterologous microsatellites in Piracanjuba

Abstract: The objective of this work was to evaluate 41 microsatellite markers for heterologous amplifications in piracanjuba (Brycon orbignyanus). Some markers were tested for the first time. Loci were optimized for PCR conditions and applied to a sample of 49 individuals. Thirty-one loci resulted in PCR product formation, whereas ten loci yielded intelligible polymorphic patterns in the evaluated sample and can be used for amplifications in this species. From the evaluated markers, four loci (BoM1, BoM13, Bh6, and Bh1… Show more

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Cited by 9 publications
(9 citation statements)
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“…LOPERA-BARRERO et al (2016b) detected amplification in Leporinus elongatus with primers of B. opalinus (BoM5) and P.lineatus (Pli43 and Pli60). CARMO et al (2015) reported positive results for B. orbignyanus by primers of Salminus brasiliensis and S. franciscanus. However, transferability between genera or families is more difficult due to the genetic distance between specimens (PENTEADO et al, 2011;LOPERA-BARRERO et al, 2016b).…”
Section: Discussionmentioning
confidence: 99%
“…LOPERA-BARRERO et al (2016b) detected amplification in Leporinus elongatus with primers of B. opalinus (BoM5) and P.lineatus (Pli43 and Pli60). CARMO et al (2015) reported positive results for B. orbignyanus by primers of Salminus brasiliensis and S. franciscanus. However, transferability between genera or families is more difficult due to the genetic distance between specimens (PENTEADO et al, 2011;LOPERA-BARRERO et al, 2016b).…”
Section: Discussionmentioning
confidence: 99%
“…However, the use of this marker requires the previous development of loci, which do not exist for E. precatoria, and this process is laborious and expensive (Gupta et al, 2016). The conservation of microsatellite sites among related species makes it possible to transfer them to other species (Guidugli et al, 2010;Santos et al, 2011, Carmo et al, 2015, Azêvedo et al, 2016Fagundes et al, 2016). To date, 18 SSR loci have been developed for Euterpe edulis, seven of which have been shown to be amplifiable and polymorphic for E. oleracea (Gaiotto et al, 2001;Oliveira et al, 2010).…”
Section: Introductionmentioning
confidence: 99%
“…Despite its status, species-specific microsatellite markers were lacking in B. orbignyanus until recent years, which led to the use of heterologous loci for breeding, genetic diversity and conservation studies in this species (e.g. Rodriguez-Rodriguez et al, 2010;Lopera-Barrero et al, 2010, 2014Ashikaga et al, 2015;Carmo et al, 2015;Castro et al, 2017). This yields a restricted number of usable loci and can lead to increasing artefactual noise in the analyses (Carmo et al, 2015).With the unveiling of the first 29 microsatellite loci from B. orbignyanus (Arias et al, 2016), by the application of next-generation sequencing (NGS) pipelines (Yazbeck et al, 2018), more reliable genetic analysis systems for this species can now be developed, in order to accelerate the profiling of extant and cultivated stocks in this threatened species.…”
mentioning
confidence: 99%
“…Rodriguez-Rodriguez et al, 2010;Lopera-Barrero et al, 2010, 2014Ashikaga et al, 2015;Carmo et al, 2015;Castro et al, 2017). This yields a restricted number of usable loci and can lead to increasing artefactual noise in the analyses (Carmo et al, 2015).With the unveiling of the first 29 microsatellite loci from B. orbignyanus (Arias et al, 2016), by the application of next-generation sequencing (NGS) pipelines (Yazbeck et al, 2018), more reliable genetic analysis systems for this species can now be developed, in order to accelerate the profiling of extant and cultivated stocks in this threatened species. Recently, seven polymorphic microsatellite loci were also developed by means of traditional cloning methods, involving a microsatellite-enriched library prepared with hybridization capture process, and their transferability assayed, along five other new markers, in Brycon gouldingi and B. falcatus (Souza et al, 2018).…”
mentioning
confidence: 99%