Optimization of Rhizopus niveus Lipase Partitioning by an Aqueous Biphasic System

Abstract

An unconventional aqueous two‐phase system (ATPS) composed of polyethylene glycol (PEG) and sodium carbonate was developed and optimized by employing response surface methodology for separation of Rhizopus niveus lipase. A five‐level central composite design was applied to evaluate the optimal level of three process variables in order to obtain maximum lipase separation. Experimental data were analyzed by regression and a polynomial model was created which was found significant. The maximum partition coefficient was achieved with the system PEG 4000/sodium carbonate. Validation experiments confirmed the high accordance of predicted and experimental results. The optimized ATPS can be applied as a suitable cost‐effective system for lipase extraction.

Partitioning of Geotrichum candidum Lipase from fermentative crude extract by aqueous two-phase system of polyethylene glycol and sodium citrate

“…While there previously published works about using ATPS in the study of extraction/purification processes of lipases is important to note that these lipases are different in size, shape, and other characteristics and are obtained by fermentation processes involving other microorganisms [16][17][18]. In this sense, so far, there has been no report in the literature on the use of ATPS for the purification of the extract fermentation of extracellular lipase of Geotrichum candidum.…”

Fabrication of a Chitosan‐Coated Magnetic Nanobiocatalyst for Starch Hydrolysis

“…The one‐factor‐at‐a‐time optimization design is simple, but fails to seek the optimum region because the interaction effects of variables are not considered. Response surface methodology (RSM) based on central composite design (CCD) is a collection of mathematical and statistical techniques for evaluating the relative significance of a multivariable system and determining the optimum conditions for various bioprocesses 31–35.…”