Optimization of incubation time of protein Tc85 in the construction of biosensor: Is the EIS a good tool?

A Low-Cost Label-Free AFB1 Impedimetric Immunosensor Based on Functionalized CD-Trodes

“…When the antibodies were incubated for 1 or 12 h, the results indicated almost the same value of R ct , and the capacitive circle presented no inductive loop [35]. This means that one hour of incubation is enough.…”

Preparation and Characterization of Imunosensors for Disease Diagnosis

“…The manner of preparation and handling of electrodes are very important for the stability and packing of self-assembled monolayers (SAM) or films and subsequent modifications steps of the analytical methodology. On cleaning screen-printed electrodes for sensors some recommendations, before the first modification step, were previously described in the literature: washing the SPE goldbased electrode with ethanol or acetone (Ferreira et al, 2010;Navrátilová & Skaládal, 2004;Kaláb & Skládal, 1995), or surface pretreatments for the immunosensors development (Escamilla-Gomez et al, 2009). Carpini et al gave the following information about pretreatment of SPE gold-based electrodes: "Although mechanical or electrochemical cleaning of the gold surface is usually recommended, both thiol-tethered DNA probe immobilization and naphthol electrochemistry are not significantly affected by surface pretreatments.…”

“…The SPE gold-based electrode, depending on the manufacturing, is not exactly a gold electrode, so the acid treatment used for cleaning their surfaces cannot be applied. Sometimes modifications may occur mainly on the surface of the reference electrode and for this reason aggressive medium cannot be used for cleaning this type of SPE electrodes (Ferreira et al, 2010). It is important to know that the SPE used in the immunosensors construction must be in an aluminum sealed package in which each electrode is individually isolated from the atmosphere, or in special boxes also protected from the atmosphere.…”

“…The results obtained for shorter antigen incubation times may be a consequence of some partial leaching of antigen due to an unstable self-assembled monolayer formation, while those for longer incubation times may indicate a possible degradation of the modified electrode surface, with loss of layer integrity. Therefore, a detailed study to optimize the incubation time of antigen in the development of biosensors is strongly recommended (Ferreira et al, 2010). The immobilization of antibodies on solid-phase materials has been used for the development of the immunosensor and different procedures were described in the literature.…”

“…Recently, more detailed studies on the surface modification using EIS with (Ferreira et al, 2009) and without (Ferreira et al, 2010) a redox marker (Fe(CN) 6 3-/4-in the solution were performed. In the first study diffusion coefficients of the marker, R CT and C dl values were obtained and compared with data of literature for the bare gold-based SPE.…”