Optimization of &lt;i&gt;Agrobacterium tumefaciens&lt;/i&gt; mediated genetic transformation protocol for aromatic ric

Hossain, Mohammad Rashed; Hassan, Lutful; Patwary, AK; Ferdous, Mostari Jahan

doi:10.3329/jbau.v7i2.4724

Cited by 5 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2), indicating the integration of NPT-II and AnnBj2 genes. Similar observations were achieved and reported in rice (Hossain et al 2009;Sarangi et al 2011;Jami et al 2012;Ahmed et al 2018).…”

Section: Molecular Analysis Of Transgenic and Non-transgenic Plantssupporting

confidence: 90%

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Mallick

Jadhao

Rout

2020

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

The aim of the study is to develop transgenic rice with annexin genes (AnnBj2) to determine its salinity and water stress tolerance through Agrobacterium-mediated genetic transformation. The transgenic somatic embryos were developed on Murashige and Skoog medium fortified with 0.5 mg L −1 kinetin and 3.0 mg L −1 2,4-D. About 80% of the transformed somatic embryos germinated and was successfully established in the transgenic greenhouse. The transformed plantlets (T 0 and T 1 generation) were identified through PCR using NPT-II and AnnBj2 gene-specific primers as well as positive uidA reporter gene expression. Compared with non-transformed plants, the transgenic rice plants overexpressing AnnBj2 gene exhibited salt tolerance at the seedling stage. Seeds generated at T 0 and T 1 generations were further studied to determine their salinity tolerance through nutrient culture and pot culture experiments for water stress. When compared with the non-transgenic control, transgenic seeds (T 0 and T 1) had increased germination on a nutrient medium having 200 mM NaCl. The data showed that the shoot and root lengths were longer at 200 mM NaCl when compared with those of the non-transformed plants. Further, the biochemical assessment (photosynthetic pigment analysis, protein, proline content, and oxidative enzyme activity) was performed between transformed (T 0 and T 1) and non-transformed plantlets under stress. It was noted that the transformed plantlets were higher in chlorophyll and proline content as well as oxidative enzyme activity as compared with the non-transformed plants.

show abstract

Section: Molecular Analysis Of Transgenic and Non-transgenic Plantssupporting

confidence: 90%

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Mallick

Jadhao

Rout

2020

In Vitro Cell.Dev.Biol.-Plant

View full text Add to dashboard Cite

show abstract

“…The maximum kanamycin resistant plants (17.24%) were obtained from method (MT 3 ) involving colonization of callus bits for 20 minutes without injury and 3 days co-cultivation with the strain having cry2Aa gene (Table 3). Similarly, about 17.5% kanamycin resistant plants were obtained by Hossain et al (2009) with 25 min colonization period (OD 600 -0.6) and 3 days of co-cultivation. Islam et al (2015) whereas recorded the maximum 32% kanamycin resistant plants with the same conditions of 25 min colonization and 3 days co-cultivation.…”

Section: Recovery Of Putative Transgenics and Transformation Frequencymentioning

confidence: 99%

Optimization of genetic transformation method for an indica rice (Oryza sativa L.) variety Ratnagiri-711

Sawant¹,

Bhave²,

Sawardekar³

et al. 2020

IJGPB

View full text Add to dashboard Cite

A study for method optimization of Agrobacterium-mediated genetic transformation for insect resistance was carried out for a rice variety Ratnagiri-711 showing better regenerability. Three different cry genes viz. cry1Aabc, cry1Fa1 and cry2Aa with a vector system consisting of the disarmed hyper virulent Agrobacterium tumefaciens strain EHA-105 harboring pBinAR or BinBt3 were used. The respective genes were linked to CaMV35S promoter and nptII gene under control of nos promoter and terminator. Scutellum-derived callus bits and embryonic shoot apical meristem of germinating seeds were used as target tissues for callus-mediated and in planta transformation, respectively. Kanamycin screening and PCR analysis was employed for confirmation of presence of transgene. Among five methods of colonization and co-cultivation tried with three cry genes, a callus-mediated transformation method consisting of 20 minutes colonization and 3 days co-cultivation with cry2Aa gene recorded highest transformation frequency (13.79%) but minimum survival (5.27%). On the contrary, considerable transformation frequency (6.35%) with highest survival (79.42%) was observed in an in planta method employing mild injury to embryonic shoot apical meristem of germinating seeds followed by injection of Agrobacterium having cry2Aa gene followed by 15 minutes colonization and then directly sowing in pots. Among three cry genes used, the gene cry2Aa was found most effective showing more transformation frequency.

show abstract

“…Lack of high yielding, biotic stressresistant and abiotic stress tolerant variety made cultivation of aromatic rice limited gradually. Hence theimprovement of the existing genotypes in respect of yield and aroma is of paramount importance (Hossain et al, 2009). Conventional breeding method merely is not sufficient and therefore, newtechnologies like gene transfer and marker assisted breeding can improve aromatic rice productivity to meet the growing demand.The presence of genetic variability for economic traits is a key factor for improving aromatic rice productivity.…”

Section: Introductionmentioning

confidence: 99%

DNA fingerprint and diversity in aromatic rice by Gn1 gene linked SSR markers

et al. 2019

View full text Add to dashboard Cite

Number of grains per panicle is a limiting factor for the yield of aromatic rice. Toovercome this limitation, itmight behelpful to screenaromatic rice using markers linked to grain number.In this study, an elementary DNA fingerprinting database of the nine aromatic rice cultivars was built using four SSR primer pairs linked to Gn1 gene responsible for grain number in rice. For genotyping of aromatic rice cultivars, DNA was extracted from leaf samples using IRRI standard protocol. Allele scoring was done by using a computer based program Alpha Ease FC 4.0 and data were analyzed by Power Marker version 3.25 software. Nei’s genetic distance value and similarity werecomputed. From the analysis, it was found that a total of 21 alleles were detected with an average number of 5.25 alleles per locus having PIC values ranging from 0.3402 (RM5493) to 0.7883 (RM3452) and the average value 0.6629. The highest gene diversity (0.8148) was observed in loci RM3452,and the lowest gene diversity (0.3404) was observed in loci RM5493.From the study, it can be stated that all of the aromatic rice germplasm have bands of the gene that influence the grain but they showed genetic variability.Information obtained from genotyping of varieties helped to analyze the genetic diversity within and among closely related crop varieties which has the potential for crop improvement and to meet the diverse goals like producing cultivars with increased yield of aromatic rice. Progressive Agriculture 29 (4): 336-344, 2018

show abstract

Optimization of <i>Agrobacterium tumefaciens</i> mediated genetic transformation protocol for aromatic ric

Cited by 5 publications

References 6 publications

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Overexpression of annexin gene in rice (Oryza sativa L.) for salinity and water stress

Optimization of genetic transformation method for an indica rice (Oryza sativa L.) variety Ratnagiri-711

DNA fingerprint and diversity in aromatic rice by Gn1 gene linked SSR markers

Contact Info

Product

Resources

About