Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Nuutila, Anna-Maria; Hämäläinen, Jouni; Mannonen, Leena

doi:10.1016/s0168-9452(99)00202-2

Cited by 38 publications

(29 citation statements)

References 23 publications

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“…This was earlier noted in callus cultures and anther cultures of barley, and currently in barley isolated microspore cultures (Nuutila et al 2000;Wojnarowiez et al 2002;Jacquard et al 2009). In our study we did not confirm the previous reports suggesting that additional copper supplementation reduces albinism in androgenesis.…”

Section: Discussionmentioning

confidence: 94%

“…Many authors emphasise that the factor which crucially limits the efficiency of androgenesis is albinism among of regenerated plants. It would seem that, given the cellular functions performed by Cu 2+ , additional supplementation into the medium may have a beneficial impact on reducing this problem (Nuutila et al 2000;Grauda et al 2014). According to Jacquard et al (2009), an increase in CuSO 4 content in the medium has a positive effect on the density of plastids in the cells.…”

Section: Discussionmentioning

confidence: 99%

“…Studies in the field have identified QTLs in nuclear genome associated with AP production, plastid genome rearrangements identified for plants without chlorophyll and cytological features characterised for plastid inability to transform into chloroplasts (Hofinger et al 2000;Caredda et al 2004;Muñoz-Amatriain et al 2008, reviewed in Makowska & Oleszczuk 2014. The frequency of AP can be reduced by using appropriate external factors, one of them being copper ions supplemented to the pretreatment solution and induction medium (Nuutila et al 2000;Wojnarowiez et al 2002). Cu 2+ ions are among heavy metals which, in high concentrations, are toxic and mutagenic for plants.…”

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confidence: 99%

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The effect of copper on plant regeneration in barley microspore culture

Makowska¹,

Oleszczuk²,

Zimny³

2017

CZECH J GENET PLANT

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Makowska K., Oleszczuk S., Zimny J. (2017): The effect of copper on plant regeneration in barley microspore culture. Czech J. Genet. Plant Breed., 53: 17-22.Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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The effect of copper on plant regeneration in barley microspore culture

Makowska¹,

Oleszczuk²,

Zimny³

2017

CZECH J GENET PLANT

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“…Regarding COPT1 function in these cell types, copper has been shown to optimize plant regeneration yield during in vitro culture of several explants (40,41). Although the molecular mechanisms underlying these observations remain unclear, copper has been postulated to function in extracellular oxidation processes (42), which may play a key role in the early stages of development and cell proliferation.…”

Section: Discussionmentioning

confidence: 99%

The Arabidopsis Copper Transporter COPT1 Functions in Root Elongation and Pollen Development

Sancenón

Puig

Mateu‐Andrés

et al. 2004

Journal of Biological Chemistry

253

197

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Copper plays a dual role in aerobic organisms, as both an essential and a potentially toxic element. To ensure copper availability while avoiding its toxic effects, organisms have developed complex homeostatic networks to control copper uptake, distribution, and utilization. In eukaryotes, including yeasts and mammals, high affinity copper uptake is mediated by the Ctr family of copper transporters. This work is the first report on the physiological function of copper transport in Arabidopsis thaliana. We have studied the expression pattern of COPT1 in transgenic plants expressing a reporter gene under the control of the COPT1 promoter. The reporter gene is highly expressed in embryos, trichomes, stomata, pollen, and root tips. The involvement of COPT1 in copper acquisition was investigated in CaMV35S::COPT1 antisense transgenic plants. Consistent with a decrease in COPT1 expression and the associated copper deprivation, these plants exhibit increased mRNA levels of genes that are down-regulated by copper, decreased rates of 64 Cu uptake by seedlings and reduced steady state levels of copper as measured by atomic absorption spectroscopy in mature leaves. Interestingly, COPT1 antisense plants also display dramatically increased root length, which is completely and specifically reversed by copper addition, and an increased sensitivity to growth inhibition by the copper-specific chelator bathocuproine disulfonic acid. Furthermore, COPT1 antisense plants exhibit pollen development defects that are specifically reversed by copper. Taken together, these studies reveal striking plant growth and development roles for copper acquisition by high affinity copper transporters.

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“…Potassium nitrate was found to be important in the germination of plum (Prunus domestica L.) 27 while some banana has a preference for NH 4 NO 3 28 . MS medium has a high concentration of NH 4 NO 3 , which is a source of NH Nitrate-N induces the activity of nitrate reductase enzyme that reduces nitrate to ammonium, which is then incorporated into proteins 29,30 . Additionally, the form of inorganic nitrogen and ammonium to nitrate ratio has been reported to greatly affect the growth and differentiation of cultured tissues 31,32 .…”

Section: Discussionmentioning

confidence: 99%

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Thawaro¹,

Te-chato²

2010

ScienceAsia

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Nutritional requirements for germination of mature zygotic embryos of four genotypes of Elaeis guineensis Jacq. Dura × Pisifera were assessed. A factorial experiment was conducted with four genotypes from separate crossing events and three culture media to show Interactions between genotypes and culture media on seedling germination (p < 0.05). The highest frequency of normal seedling germination was facilitated by 1/2 Murashige and Skoog (MS) medium (29.2%), followed by MS (15.0%) and Blaydes (12.9%). Speed of shoot formation followed a similar trend. Roots were induced most rapidly in 1/2 MS medium, followed by Blaydes and then MS. Root induction was similar in 1/2 MS medium (2.29%) and Blaydes medium (1.25%) but only significantly higher than in MS (0.00%).

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Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.)

Cited by 38 publications

References 23 publications

The effect of copper on plant regeneration in barley microspore culture

The effect of copper on plant regeneration in barley microspore culture

The Arabidopsis Copper Transporter COPT1 Functions in Root Elongation and Pollen Development

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