Optimization of poly(amido amine)s as vectors for siRNA delivery

Aa, L.J. van der; Vader, Pieter; Storm, Gert; Schiffelers, Raymond M.; Engbersen, Johan F.J.

doi:10.1016/j.jconrel.2010.11.030

Cited by 48 publications

(40 citation statements)

References 26 publications

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“…A viable non-viral vector should be able to protect genes from complex biological environment, improve cellular uptake and facilitate endosomal escape. Due to the anionic nature of genes, various synthetic cationic polymers, such as poly(ethyleneimine) (PEI), [51] poly(amido amine) (PAA), [52] poly(β-amino ester) (PAE), [53] polypeptide, [54] poly(dimethylaminoethyl methacrylate) (PDMAEMA) [55] and some natural polymers, such as chitosan [56] and cationic starches [57] have been studied and used to form complex with genes through electrostatic interactions to fabricate gene delivery systems.…”

Section: Co-delivery Of Chemical Drugs and Genesmentioning

confidence: 99%

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Thambi

Lee

2017

Adv Healthcare Materials

107

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Drug and gene delivery systems based on nanoparticles, microparticles and hydrogels have been widely studied for cancer treatment in the past decade. To achieve an efficient and safe delivery, selection of drug and gene delivery carrier is critical. Biocompatible polymeric nanoparticles are considerably promising carrier candidates in delivery of drugs and genes because of their unique chemical and physical properties. However, delivery of a drug or gene sometimes cannot achieve a satisfactory treatment effect. Therefore, co‐delivery of dual drugs or co‐delivery of a drug and a gene in a polymeric nanoparticle has attracted attention. Such co‐delivery systems can overcome multi‐drug resistance of chemical drugs and achieve a synergistic therapeutic effect. In this progress report, we summarize recent progress in the preparation and application of polymeric drug and gene co‐delivery nanosystems. The remaining challenges and future trends in this field are also included.

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Section: Co-delivery Of Chemical Drugs and Genesmentioning

confidence: 99%

Co‐Delivery of Drugs and Genes Using Polymeric Nanoparticles for Synergistic Cancer Therapeutic Effects

Thambi

Lee

2017

Adv Healthcare Materials

107

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“…25 Polymerization was conducted in vials containing a methanol/ water mixture (9/1, v/v) at 35°C for a period of 4 days. The reaction conditions and results are shown in Table 1.…”

Section: Synthesis Of Dual-responsive Paasmentioning

confidence: 99%

Bioreducible and acid-labile poly(amido amine)s for efficient gene delivery

Yu¹,

Yan²,

You³

et al. 2012

IJN

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Intracellular processes, including endosomal escape and intracellular release, are efficiency-determining steps in achieving successful gene delivery. It has been found that the presence of acid-labile units in polymers can facilitate endosomal escape and that the presence of reducible units in polymers can lead to intracellular release. In this study, poly(amido amine)s with both bioreducible and acid-labile properties were synthesized to improve gene delivery compared with single-responsive carriers. Transfection and cytotoxicity were evaluated in three cell lines. The complexes of DNA with dual-responsive polymers showed higher gene transfection efficiency than single-responsive polymers and polyethylenimine. At the same time, these polymers were tens of times less cytotoxic than polyethylenimine. Therefore, a polymer that is both reducible and acid-labile is a promising material for efficient and biocompatible gene delivery.

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“…therapeutic activity. 3 Currently, carriers for siRNA delivery include viral and nonviral carriers, such as peptides, 4 cationic lipids, 5 and cationic polymers, such as polyethylenimine (PEI), 6 and poly-l-lysine. 7 In addition, carriers based on inorganic nanoparticles, such as silica nanoparticles, are also members of the nonviral carriers.…”

Section: Introductionmentioning

confidence: 99%

“…For cancer therapy, in order to reach distant tumors or metastases, systemic administration of siRNA polyplexes is inevitable. 3 Upon intravenous injection, however, positively charged polyplexes might interact nonspecifically with serum proteins or erythrocytes and other blood cells, leading to the formation of aggregates, which causes rapid clearance by the reticuloendothelial system and sometimes significant toxicity. 18 The biocompatibility of polyplexes can be enhanced by conjugation of polyethylene glycol (PEG) to the cationic polymer (PEGylation).…”

Section: Introductionmentioning

confidence: 99%