Optimization of separation of a complex mixture of natural and synthetic corticoids by micellar liquid chromatography using sodium dodecyl sulphate

Mixture‐designed electrolytes for the MEKC separation of natural and synthetic steroids

“…The extraction procedure was adapted from the literature [11]: to a 3.00 mL portion of thawed membrane-filtered sample, 0.35 g NaCl, 0.5 g Na 2 HPO 4 (to raise the pH to 9.0), and 4.00 mL dichloromethane were added; the organic layer was removed, dried with anhydrous Na 2 SO 4 , evaporated to dryness, reconstituted into 50 mL methanol, and diluted with 450 mL 5 mmol/L SDS/5 mmol/L tetraborate solution prior to injection.…”

“…For confirmatory testing, chromatographic techniques such as GC and LC, especially when coupled to MS, are usually preferred [2,[7][8][9]. The unique selectivity of micellar LC (MLC) systems has been demonstrated for anabolic steroids and corticosteroids determination [10,11] and as a means to mimicking membrane structure [12]. In gen- eral, these approaches require qualified operators, complex instrumentation, whose maintenance is relatively costly, and often samples must be derivatized prior to injection.…”

Optimization of the separation of a complex mixture of natural and synthetic anabolic steroids by micellar liquid chromatography

“…In previous papers, the optimization of the separation of a complex mixture of natural and synthetic corticoids [17] and urinary steroids (corticoids and anabolics) [18] in MLC using SDS and different organic modifiers has been reported. To improve column efficiency, higher temperatures and smaller flow-rates than those used in HPLC were proposed.…”

“…MLC has been employed for separation studies of complex mixtures of corticoids, urinary steroids (corticoids and anabolics) [17,18] and for the determination of single anabolic compounds in pharmaceuticals [9].…”

Determination of some banned stimulants in sports by micellar liquid chromatography

“…Micellar liquid chromatography (MLC) has proved to be a useful technique in the determination of diverse groups of drugs in urine, such as barbiturates [12], natural and synthetic corticosteroids [13], 13-antagonists [14], steroids [15] and nicotine and cotidine [16].…”