2021
DOI: 10.13057/biodiv/d220702
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Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes

Abstract: Abstract. Carsono N, Juwendah E, Liberty, Sari S, Damayanti F, Rachmadi M. 2021. Optimize 2,4-D concentration and callus induction time enhance callus proliferation and plant regeneration of three rice genotypes. Biodiversitas 22: 2555-2560. The development of callus in the course of transgenic rice avoids the somaclonal variants. To obtain a high number of normal phenotypes and a low number of somaclonal variants requires an appropriate 2,4-D concentration. In this study, we obtained the best callus induction… Show more

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Cited by 15 publications
(10 citation statements)
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“…The formation of calli is very important to regenerate adventitious buds. Calluses of different ages showed different growth capacities and regeneration abilities ( Cheng et al., 2009 ; Carsono et al., 2021 ), which was also shown in our study. The highest frequency of callus and adventitious bud induction of E. urophylla × E. grandis DH32-29 was obtained when leaves were used to induce calluses for 15 days.…”
Section: Discussionsupporting
confidence: 88%
“…The formation of calli is very important to regenerate adventitious buds. Calluses of different ages showed different growth capacities and regeneration abilities ( Cheng et al., 2009 ; Carsono et al., 2021 ), which was also shown in our study. The highest frequency of callus and adventitious bud induction of E. urophylla × E. grandis DH32-29 was obtained when leaves were used to induce calluses for 15 days.…”
Section: Discussionsupporting
confidence: 88%
“…Auxins cause embryogenic and organogenic differentiation, cytodifferentiation, and cell division in tissue culture [ 24 ]. Among PGRs, the auxin 2,4-D (dichlorophenoxyacetic acid) is well-known for helping to accelerate the proliferation and expansion of embryogenic calli [ 25 ]. Many modifications in both type and concentration of PGRs have been evaluated.…”
Section: Introductionmentioning
confidence: 99%
“…The medium contain 1 mg/L 2,4-D + 1 mg/L BAP (ACM1) generates the greatest calli weight (2.086 grams). The involvement of 2,4-D is to promote the mechanism of morphogenesis of calli, calli initiation, and to regulate the genetic structure of the plant tissues [16,17]. In this treatment, the use of endogenous or exogenous auxin and cytokinin promotes regeneration [18,19,20].…”
Section: Characteristics Of Calli Formationmentioning
confidence: 99%