Optimized depth-resolved estimation to measure optical attenuation coefficients from optical coherence tomography and its application in cerebral damage determination

et al. 2019

Neurophoton.

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edema detection in vivo after middle cerebral artery occlusion using swept-source optical coherence tomography,"Abstract. Cerebral edema is a severe complication of ischemic cerebrovascular disease, which can lead to microcirculation compression resulting in additional ischemic damage. Real-time and continuous in vivo imaging techniques for edema detection are of great significance to basic research on cerebral edema. We attempted to monitor the cerebral edema status in rats with middle cerebral artery occlusion (MCAO) over time, using a wide field-of-view swept-source optical coherence tomography (SS-OCT) system. Optical attenuation coefficients (OACs) were calculated by an optimized depth-resolved estimation method, and en face OAC maps covering the whole cortex were obtained. Then, the tissue affected by edema was segmented from the OAC maps, and the cortical area affected by edema was estimated. Both magnetic resonance image (MRI) and brain water content measurements were used to verify the presence of cerebral edema. The results showed that the average OAC of the ischemic area gradually decreased as cerebral edema progressed, and the edema area detected by SS-OCT had high similarity in position and shape to that obtained by MRI. This work extends the application of OCT and provides an option for detecting cerebral edema in vivo after ischemic stroke. © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

Section: Calculation Of Optical Attenuation Coefficientmentioning

confidence: 99%

Section: -5mentioning

confidence: 99%

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Cerebral edema detection in vivo after middle cerebral artery occlusion using swept-source optical coherence tomography

et al. 2019

Neurophoton.

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“…In this study, a spectral‐domain OCT (SD‐OCT) system with a central wavelength of 1310 nm and a spectral bandwidth of 60 nm was used to visualize the femoral vein and for all acquisitions as previously described . Briefly, the spectrometer used A line scan infrared InGaAs array detector operating at a 92 kHz line rate, and for each cross‐sectional image 400 A lines were collected.…”

Section: Methodsmentioning

confidence: 99%

In vivo monitoring of thrombosis in mice by optical coherence tomography

Journal of Biophotonics

et al. 2019

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The objective of this study is to establish a novel method for continuously monitoring thrombus progression with various outcome measures and to assess the efficacy of antithrombotic drugs in murine thrombosis model in mice. In the study, thrombus was induced in the femoral vein of mice by FeCl 3 and monitored over time by spectraldomain optical coherence tomography (OCT). Three-dimensional images of thrombi with or without heparin as an antithrombotic agent were obtained from OCT angiography. In addition, several parameters of thrombi were analyzed and compared between control and anticoagulant groups. By using OCT, we were able to trace thrombus generation in the same mouse in real time. We found that in our model heparin reduced thrombus size by~60% and thrombus cross-sectional area by 50%. OCT results also show that both time to thrombus size (>0.02mm 3 ) and time to occlusion (>30%) were significantly reduced after heparin addition. This study demonstrates that OCT reliably monitors thrombus generation and progression from various aspects including thrombus size. This enables us to measure the kinetic of thrombosis more accurately, and effectively evaluate the efficacy and activities of antithrombotic drugs.This model may represent a useful tool in antithrombotic drug discoveries in preclinical studies. K E Y W O R D Santithrombotic drugs, mice, optical coherence tomography, thrombosis

“…An optimized depth-resolved estimation method was used to calculate OAC [36]. Then, a 3D matrix was reconstructed from the cortex surface, extending a specific number of pixels vertically.…”

Section: Calculation Of Oacmentioning

confidence: 99%

Simultaneous detection of cerebral blood perfusion and cerebral edema using swept‐source optical coherence tomography

Journal of Biophotonics

et al. 2019

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The progression of ischemic cerebral edema (CE) is closely related to the level of cerebral blood perfusion (CBP) and affects each other.Simultaneous detection of CBP and CE is helpful in understanding the mechanisms of ischemic CE development. In this article, a wide field of view swept-source optical coherence tomography system was used to detect CE status and CBP levels simultaneously in middle cerebral artery occlusion rats. Images reflecting these two physiological states can be reconstructed with only one C-scan. We quantify these two physiological states into four parameters, which contain two vascular parameters (vascular displacement distance and vascular perfusion density) and two edema parameters (optical attenuation coefficient and edema area). The association between the two vascular parameters and the two edema parameters was analyzed. The results show that there is a strong linear relationship between blood flow parameters and edema parameters. This work provides a new option for CE in vivo detection, and is very likely to play an important role in the development of relevant drugs or in selection of treatment options. K E Y W O R D Scerebral blood perfusion, cerebral edema, middle cerebral artery occlusion, optical attenuation coefficient, swept-source optical coherence tomography