Oral administration of Brazilian propolis exerts estrogenic effect in ovariectomized rats

Okamoto, Yoshinori; Tobe, Takao; Ueda, Koji; Takada, Tatsuyuki; Kojima, Nakao

doi:10.2131/jts.40.235

Cited by 16 publications

(27 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our hypothesis is that the neonatal chronic stress induces the apoptosis of granulosa cells and may reduce 17-beta estradiol biosynthesis, and consequently reduce the quality of the ovum. Our results are in agreement with the findings reported by Okamoto et al , showed that oral administration of propolis induces estrogenic activity in estrogen target organs in vivo suggesting that propolis is a useful dietary source of phytoestrogens and a promising treatment for post-menopausal symptoms ( 33 ).…”

Section: Discussionsupporting

confidence: 93%

“…The several reports have shown that the ovaries of the female rats contain only the oocytes immediately after birth and are without any follicles, and this means that follicular development and evolution process occurs after the birth in the rats ( 29 , 33 ). As well, it is known that primordial follicles are created three days after the birth and lead to the formation of primary follicles immediately after their growth and differentiation process ( 32 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress

Arabameri

Sameni

Bandegi

2017

IJRM

View full text Add to dashboard Cite

Background:Stress in infancy has dramatic effects on different systems, including the nervous system, endocrine, immune, reproductive and etc.Objective:The purpose of this study was to investigate the effects of extract of Iranian propolis (EIP) on ovarian tissue and oxidative stress in rats with maternal separation stress.Materials and Methods:48 immature female rats were divided randomly into six groups. 1) Control group, 2) Control group+saline, 3) Stress group, includes infants that were separated from their mothers 6 hr/day, the 4th, 5th and 6th groups consisted of infants who in addition to daily stress received 50, 100 and 200 mg/kg of EIP, respectively. Then serum corticosterone, 17-beta-estradiol, malondialdehyde, total superoxide dismutase, glutathione peroxidase and ferric reducing antioxidant power levels were measured. The ovarian sections were stained by H&E, PAS, and TUNEL methods and were studied with optical microscopy. Results:Stress increased the blood serum corticosterone levels and 17-beta-estradiol reduced significantly (p<0.001) and EIP prevented from this changes (p<0.01). EIP significantly increased the number of ovarian follicles, oocytes and oocytes diameter in neonatal rat following stress (p<0.01). EIP also significantly decreased the number of atretic follicles, TUNEL+granulosa cells, malondialdehyde levels and increased ferric reducing antioxidant power, total superoxide dismutase and glutathione peroxidase serum levels in neonatal rats following stress. The dose of 200 mg/kg EIP was more effective.Conclusion:This Study showed that the Iranian Propolis significantly could prevent oxidative stress and histopathological changes in the ovary of the neonatal rat the following stress.

show abstract

Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress

Arabameri

Sameni

Bandegi

2017

IJRM

View full text Add to dashboard Cite

show abstract

“…Chemical composition of propolis greatly varies according to the plants that can be found in a specific region [18, 19]. The caffeic acid derivatives has been detected as potential estrogenic components in Cameroonian and Korean propolis, while Okamoto et al [42] attributed the estrogenic effects of Brazilian propolis to the well-known phytoestrogens Kaempferol, quercetin, naringenin, Biochanin A and formononetin. These aforesaid suggest a regional difference in estrogenic components of propolis.…”

Section: Discussionmentioning

confidence: 99%

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Zingue

Nde

Michel

et al. 2017

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundSince the biological properties of propolis depend to the plants that can be found in a specific region, propolis from unexplored regions attracts the attention of scientists. Ethanolic extract of Cameroonian propolis (EEP) is used to treat various ailments including gynecological problems and amenorrhea. Since there were no scientific data to support the above claims, the present study was therefore undertaken to assess estrogenic properties of Cameroonian propolis.MethodsTo achieve our goal, the ability of EEP to induce MCF-7 cells proliferation in E-screen assay as well as to activate estrogen receptors α (ERα) and β (ERβ) in cell-based reporter gene assays using human embryonic kidney cells (HEK293T) transfected with ERs was tested. Further, a 3-day uterotrophic assay was performed and the ability of EEP to alleviate hot flushes in ovariectomized adult rats was evaluated.ResultsIn vitro, EEP showed an antiestrogenic activity in both HEK293T ER-α and ER-β cells. In vivo, EEP induced a significant increase in a bell shape dose response manner of the uterine wet weight, the total protein levels in the uterus, the uterine and vaginal epithelium height and acini border cells of mammary gland with the presence of abundant eosinophil secretions. Moreover, EEP induced a significant decrease in the total number, average duration as well as frequency of hot flushes after 3 days of treatment in rat (equivalent to a month in woman). The dose of 150 mg/kg exhibited the most potent estrogenic effects among all the tested doses. The UPLC-HRMS analysis showed the presence of caffeic acid derivatives and trirtepernoids in EEP, which are well known endowed with estrogenic properties.ConclusionThese results suggest that Ethanolic extract of Cameroonian propolis has estrogen-like effects in vivo and may alleviate some menopausal problems such as vaginal dryness and hot flushes.Graphical abstractEthanol-extracted Cameroobian propolis exhibited in vitro and in vivo estrogen-like effects. This extract may contain promising phytoestrogens.

show abstract

“…There was a non-significant increase of uterine tumors incidence in high dose female group, and we concluded that in the present conditions, 2.5% EEP is likely to exert estrogenic activity but lacks the carcinogenicity in the uterus of Wistar Hannover rats. Previously, the estrogenic effects of propolis were demonstrated through the activation of an estrogen receptor [ 26 ]. For instance, EEP was found to bind human estrogen receptors and to induce the expression of estrogen-responsive genes in ER-positive MCF-7 and Ishikawa cells and to induce estrogenic activity in ER-expressing organs of ovariectomized rats.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, ethanol-extracted Brazilian green propolis (EEP) exerted promoting effect on F344 rat bladder carcinogenesis initiated with N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) [ 23 ]. Moreover, it was recently found to exert estrogenic effect in ovariectomized rats, significantly increasing uterine luminal epithelium thickness and ductal cell proliferation in the mammary glands [ 26 ]. Therefore, there is a doubt concerning propolis carcinogenicity in the liver, bladder, mammary gland and uterus.…”

Section: Introductionmentioning

confidence: 99%

Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats

et al. 2016

View full text Add to dashboard Cite

The present study was conducted over a course of 104 weeks to estimate the carcinogenicity of ethanol-extracted Brazilian green propolis (EEP). Groups of 50 male and 50 female Wistar Hannover rats, 6-week-old at commencement were exposed to EEP at doses of 0, 0.5 or 2.5% in the diet. Survival rates of 0.5% and 2.5% EEP-treated male and female rats, respectively, were significantly higher than those of respective control groups. Overall histopathological evaluation of neoplasms in rat tissues after 2 years showed no significant increase of tumors or preneoplastic lesions in any organ of animals administered EEP. Significantly lower incidences of pituitary tumors in 0.5% EEP male and 2.5% EEP female groups, malignant lymphoma/leukemia in both 2.5% EEP-treated males and females and total thyroid tumors in 0.5% EEP male group were found. Administration of EEP caused significant decreases of lymphoid hyperplasia of the thymus and lymph nodes in 2.5% EEP-treated rats, tubular cell hyperplasia of kidneys in all EEP groups, and cortical hyperplasia of adrenals in EEP-treated females. In the blood, significant reduction of neutrophils in all EEP-treated males and band neutrophils in 2.5% EEP-treated females was found indicating lower levels of inflammation. Total cholesterol and triglicerides levels were significantly lower in the blood of 2.5% EEP-treated female rats. In conclusion, under the conditions of the 2-year feeding experiment, EEP was not carcinogenic, did not induce significant histopathological changes in any organ, and further exerted anti-inflammatory and antitumorigenic effects resulting in increase of survival of Wistar Hannover rats.

show abstract

Oral administration of Brazilian propolis exerts estrogenic effect in ovariectomized rats

Cited by 16 publications

References 22 publications

The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress

The effects of propolis extract on ovarian tissue and oxidative stress in rats with maternal separation stress

Ethanol-extracted Cameroonian propolis exerts estrogenic effects and alleviates hot flushes in ovariectomized Wistar rats

Ethanol-Extracted Brazilian Propolis Exerts Protective Effects on Tumorigenesis in Wistar Hannover Rats

Contact Info

Product

Resources

About