Oral administration of dibutyryl adenosine cyclophosphate improved growth performance in weaning piglets by enhancing lipid fatty acids metabolism

Li, Guanya; Chang, Ling; Zhang, Guanglei; Song, Zehe; Wan, Dan; Xie, Changqing; Wang, Hong; Fan, Zhiyong

doi:10.1016/j.aninu.2018.06.002

Cited by 4 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The prawns fed with a 100 mg/kg TTO diet improved the growth indicators (WGR, SGR, FCR) while decreasing the HSI compared to the control. Decreased HSI may be associated with enhanced hepatic lipid energy metabolism and the energy used for the growth of other tissues [ 38 ]. Therefore, combined with our undisclosed results about muscles, we speculated that the 100 mg/kg TTO diet may promote the other tissues’ growth (e.g., muscles) instead of the hepatopancreas.…”

Section: Discussionmentioning

confidence: 99%

Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii

Zheng

Sun

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Both oxidative stress and autophagy refer to regulating fat metabolism, and the former affects autophagy, but the role and mechanism of the antioxidant–autophagy axis in regulating lipid metabolism remains unclear. As an antioxidant, tea tree oil (TTO) has little research on the regulatory mechanism of lipid metabolism in crustaceans. This study investigated whether TTO could alter hepatopancreatic lipid metabolism by affecting the antioxidant–autophagy axis. Feed Macrobrachium rosenbergii with three different levels of TTO diets for 8 weeks: CT (0 mg/kg TTO), 100TTO (100 mg/kg TTO), and 1000TTO (1000 mg/kg TTO). The results showed that 100TTO treatment reduced the hemolymph lipids level and hepatopancreatic lipid deposition compared to CT. In contrast, 1000TTO treatment increased hepatopancreatic lipid deposition, damaging both morphology and function in the hepatopancreas. The 100TTO treatment promoted lipolysis and reduced liposynthesis at the transcriptional level compared to the CT group. Meanwhile, it improved the hepatopancreas antioxidant capacity and maintained mitochondrial structural and ROS homeostasis. In addition, it simultaneously activated the expression of transcription factors Keap1-Nrf2 and Imd-Relish. By contrast, the 1000TTO group significantly enhanced the ROS level, which considerably activated the Keap1-Nrf2 signaling expression but had no significant effects on the expression of Imd-Relish. The 100TTO group supplementation significantly enhanced lipid droplet breakdown and autophagy-related genes and protein expression. On the contrary, the 1000TTO group significantly inhibited the expression of genes and proteins related to autophagy. Pearson analysis revealed that Nrf2 has a positive correlation to lipid anabolism-related genes (Fasn, Srebp1, Pparγ) and autophagy regulators (mtor, akt, p62), and were negatively correlated with lipolysis-related genes (Cpt1, Hsl, Ampkα) and autophagy markers (Ulk1, Lc3). Relish was positively correlated with Atgl, Cpt1, Ampkα, Ulk1, and Lc3, and negatively correlated with Pparγ and p62. Moreover, Keap1 and Imd were negatively correlated with p62 and mtor, respectively. In sum, 100 mg/kg TTO enhanced antioxidant activity and increased autophagy intensity through the Relish-Imd pathway to enhance lipid droplet breakdown, while 1000 mg/kg TTO overexpressed Nrf2, thus inhibiting autophagy and ultimately causing excessive lipid deposition and peroxidation. Our study gives a fresh perspective for deciphering the bidirectional regulation mechanism of lipid metabolism by different doses of TTO based on the antioxidant–autophagy axis.

show abstract

Section: Discussionmentioning

confidence: 99%

Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii

Zheng

Sun

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

Dietary dibutyryl cAMP supplementation regulates the fat deposition in adipose tissues of finishing pigs via cAMP/PKA pathway

Zhu

Wang

Nie

et al. 2021

Animal Biotechnology

View full text Add to dashboard Cite

Weaning Stress in Piglets Alters the Expression of Intestinal Proteins Involved in Fat Absorption

Liu

et al. 2022

The Journal of Nutrition

View full text Add to dashboard Cite

Background In vivo data on intestinal fat absorption in weanling piglets are scarce. Objective This study aimed to investigate the effect of weaning stress on intestinal fat absorption. Methods 18 seven-day-old sow-reared piglets (Duroc-Landrace-Yorkshire) were assigned to 3 groups (n = 6/group, 3 males and 3 females per group). Piglets were nursed by sows until 24 d of age (suckling piglets, S), or weaned at 21 d of age to a corn-soybean meal-based diet until 24 d (3 d post-weaning, W 3) or 28 d (7 d post-weaning, W 7) of age, respectively. Duodenum, jejunum, and ileum were collected to determine intestinal morphology and abundance of proteins related to fat absorption. Result Compared with the S group, the W 3 group had lower villus height (17%-34%) and villus height to crypt depth ratio (13%-53%), as well as a 1-1.45 times greater crypt depth, which in the W7 group was 1.18-1.31, 0.69-1.15, and 1.47-1.87 times greater than in the W 3 group, respectively. Compared with the S group, weaning stress for both 3 and 7 d reduced intestinal alkaline phosphatase activity (26%-73%), serum lipids (26%-54%), and abundances of proteins related to fatty acid transport (FATP4 and I-FABP) and chylomicron assembly (APOA4, MTTP, APOB, and APOA1) in the duodenum and ileum (10%-55%), as well as in the jejunum (25%-85%). All these indexes did not differ between W 3 and W 7 groups. Compared with the S group, the W 3 group had lower mRNA levels of duodenal APOA4 and APOA1 (25%-50%), as well as jejunal FATP4, IFABP, MTTP, APOA4, and APOA1 (35%-50%), whereas they were 5-15% and 10-37% lower in the W 7 group than in the W 3 group, respectively, Conclusion Weaning stress in piglets attenuates the expression of intestinal proteins related to fatty acid transport (FATP4 and I-FABP) and chylomicron synthesis (APOA4).

show abstract

Oral administration of dibutyryl adenosine cyclophosphate improved growth performance in weaning piglets by enhancing lipid fatty acids metabolism

Cited by 4 publications

References 36 publications

Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii

Tea Tree Oil Mediates Antioxidant Factors Relish and Nrf2-Autophagy Axis Regulating the Lipid Metabolism of Macrobrachium rosenbergii

Dietary dibutyryl cAMP supplementation regulates the fat deposition in adipose tissues of finishing pigs via cAMP/PKA pathway

Weaning Stress in Piglets Alters the Expression of Intestinal Proteins Involved in Fat Absorption

Contact Info

Product

Resources

About