Oral administration of Lactococcus chungangensis inhibits 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in NC/Nga mice

Choi, Woo Jin; Konkit, Maytiya; Kim, Yena; Kim, Mi-Kyung; Kim, Wonyong

doi:10.3168/jds.2016-11301

Cited by 21 publications

(17 citation statements)

References 69 publications

(66 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we investigated cytokines related to Th2 in an in vivo model. Jawoongo treatment reduced the increased serum levels of IgE, IL-6, IL-10 and IL-12 induced by DNCB treatment In fact, it was reported that IL-12 was increased by DNCB [ 46 , 47 ], and this increase was suppressed by Jawoongo as expected. We also found that Jawoongo reduced DNCB-stimulated increases in eosinophil, neutrophil, monocyte, basophil, lymphocyte and WBC numbers and in IL-4, IL-13 and TNF-α mRNA expression.…”

Section: Discussionsupporting

confidence: 60%

The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo

Hong

Kim

et al. 2018

BMC Complement Altern Med

View full text Add to dashboard Cite

BackgroundJawoongo is an herbal mixture used in traditional medicine to treat skin diseases. This study aimed to investigate whether Jawoongo ameliorates Atopic dermatitis (AD)-like pathology in mice and to understand its underlying cellular mechanisms.MethodsAD was induced by 2, 4-Dinitrocholrlbenzene (DNCB) in BALB/c mice. Treatment with Jawoongo was assessed to study the effect of Jawoongo on AD in mice. Histological Analysis, blood analysis, RT-PCR, western blot analysis, ELISA assay and cell viability assay were performed to verify the inhibitory effect of Jawoongo on AD in mice.ResultsWe found that application of Jawoongo in an ointment form on AD-like skin lesions on DNCB-exposed BALB/c mice reduced skin thickness and ameliorated skin infiltration with inflammatory cells, mast cells and CD4+ cells. The ointment also reduced the mRNA levels of IL-2, IL-4, IL-13 and TNF-α in the sensitized skin. Leukocyte counts and the levels of IgE, IL-6, IL-10 and IL-12 were decreased in the blood of the DNCB-treated mice. Furthermore, studies on cultured cells demonstrated that Jawoongo exhibits anti-inflammatory activities, including the suppression of proinflammatory cytokine expression, nitric oxide (NO) production, and inflammation-associated molecule levels in numerous types of agonist-stimulated innate immune cell, including human mast cells (HMC-1), murine macrophage RAW264.7 cells, and splenocytes isolated from mice.ConclusionThese findings indicate that Jawoongo alleviates DNCB-induced AD-like symptoms via the modulation of several inflammatory responses, indicating that Jawoongo might be a useful drug for the treatment of AD.Electronic supplementary materialThe online version of this article (10.1186/s12906-018-2280-z) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussionsupporting

confidence: 60%

The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo

Hong

Kim

et al. 2018

BMC Complement Altern Med

View full text Add to dashboard Cite

show abstract

“…29 Several studies have shown that a probiotic, Lactococcus chungangensis, could improve atopic dermatitis symptoms in a COM-induced mouse model. 30 Using this model, we observed that a composite mixture of four species of probiotic bacteria and sodium butyrate mitigated atopic dermatitis symptoms due to histamine secretion. Atopic dermatitis is characterized by Th1/Th2 cells imbalance and dominant Th2 cells-mediated responses.…”

Section: Discussionmentioning

confidence: 95%

Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model

Kim

et al. 2018

Journal of Medicinal Food

View full text Add to dashboard Cite

Atopic dermatitis is a chronic and recurrent inflammatory skin disease. Recently, probiotics have been shown to suppress allergic symptoms through immunomodulatory responses. In the present study, combinatorial effects on allergic symptoms were identified in BALB/c mice fed with a mixture of four species of probiotics, Bifidobacterium lactis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus plantarum, and sodium butyrate. Following sensitization with whey protein, the mice were challenged and divided into two groups: (1) mice administered with phosphate-buffered saline as a control and (2) mice administered with the probiotic mixture and sodium butyrate. Allergic symptoms were assessed by measuring ear thicknesses, serum histamine and IL-10 concentrations, and the quantities of leaked Evans blue. T cell differentiation was determined by analyzing the T cells groups in the mesenteric lymph nodes (MLNs) and spleen. To examine changes in the total gut microbiota, total fecal microflora was isolated, species identification was performed by DNA sequencing using Illumina MiSeq, and changes in intestinal beneficial bacteria were analyzed using quantitative polymerase chain reaction. Treatment with the probiotic mixture and sodium butyrate reduced ear thicknesses, the quantity of leaked Evans blue, and serum histamine values, while increasing serum IL-10 values. In the mouse model, the probiotic mixture and sodium butyrate increased Th1 and Treg cell differentiation in MLN and spleen tissues; the ratio of Firmicutes/Bacteroidetes, which is associated with reduction in allergic reactions; and microorganisms that lead to cell differentiation into Treg. These results suggest that the probiotic mixture and sodium butyrate can prevent and alleviate allergic symptoms.

show abstract

“…Probiotic bacteria can interact with the host mucosa and may beneficially modulate immune system (Choi et al . ). Previously, clinical trials involving in vivo animal models have reported that various types of LAB alleviate allergic symptoms (Kalliomaki et al .…”

Section: Discussionmentioning

confidence: 97%

“…; Choi et al . ). Lactobacillales or lactic acid bacteria (LAB) produce lactic acid as a metabolic end‐product, and are known to prevent AD symptoms (Masuda et al .…”

Section: Introductionmentioning

confidence: 97%

Lactococcus lactisKR-050L extract suppresses house dust mite induced-atopic skin inflammation through inhibition of keratinocyte and mast cell activation

et al. 2018

View full text Add to dashboard Cite

Aims: Atopic dermatitis (AD) is a chronic inflammatory skin disease, with a steadily increasing prevalence. Lactic acid bacteria (LAB) have been widely used in the food industry and are an attractive option for preventing and treating allergic skin diseases. We previously isolated new LABs including Lactococcus lactis KR-050L from Gajuknamu kimchi, and showed the anti-inflammatory effects of extract of L. lactis KR-050L culture broth (LLK). In this study, we investigated the effects of LLK on AD. Methods and Results: For the in vitro study, we used human keratinocytes (HaCaT) and mast cells (RBL-2H3). In vivo study, we investigated the effects of LLK on Dermatophagoides farinae extract (DFE) and 2,4-dinitrochlorobenzene (DNCB)-induced atopic skin inflammation in mice. LLK suppressed expression of pro-inflammatory cytokines and chemokines by down-regulation of p38 MAPK, STAT1 and nuclear translocation of NF-jB in keratinocytes. Topical application of LLK suppressed AD symptoms based on reduction in ear thickness, serum IgE levels and immune cell infiltration. Furthermore, LLK inhibited serum histamine levels and mast cells infiltration in vivo, and reduced mast cells activation in vitro. Conclusions: These results suggest that LLK inhibits AD symptoms through inhibition of keratinocytes and mast cells activation. Significance and Impact of the Study: LLK is a potential therapeutic candidate for AD treatment.

show abstract

Oral administration of Lactococcus chungangensis inhibits 2,4-dinitrochlorobenzene-induced atopic-like dermatitis in NC/Nga mice

Cited by 21 publications

References 69 publications

The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo

The prevention of 2,4-dinitrochlorobenzene-induced inflammation in atopic dermatitis-like skin lesions in BALB/c mice by Jawoongo

Anti-Inflammatory Effects of a Mixture of Lactic Acid Bacteria and Sodium Butyrate in Atopic Dermatitis Murine Model

Lactococcus lactisKR-050L extract suppresses house dust mite induced-atopic skin inflammation through inhibition of keratinocyte and mast cell activation

Contact Info

Product

Resources

About