Oral immunization with attenuated Salmonella encoding an elastase elicits protective immunity against Trichinella spiralis infection

Zhang, Xin Zhuo; Yue, Wencong; Bai, Sheng Jie; He, Hao; Song, Yan; Long, Stuart A.; Liu, Ruo Dan; Cui, Jing; Wang, Zhong Quan

doi:10.1016/j.actatropica.2021.106263

Cited by 19 publications

(21 citation statements)

References 83 publications

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“…Moreover, gut sIgA could reduce the female reproductive capacity of intestinal T . spiralis [ 9 ]. Our results revealed that the female fecundity of vaccinated mice with TsCRT+TsSP1.1 combination is significantly lower than only TsCRT or TsSP1.1 vaccination alone.…”

Section: Discussionmentioning

confidence: 99%

“…The positive transformant was selected on MacConkey Agar Medium with 50 μg/ml ampicillin and identified by PCR. The PCR products were sequenced (Sangon Biotech, Shanghai, China) to further verify the true introduction of plasmids into the bacteria and the successful construction of oral DNA vaccines [ 9 ].…”

Section: Methodsmentioning

confidence: 99%

“…Trichinella infection in food animals is a serious threat to the meat food safety [ 6 , 7 ]. Therefore, it’s necessary to develop anti- Trichinella vaccines to interrupt Trichinella infection in domestic pigs, and eliminate the ML in pork [ 8 , 9 ]…”

Section: Introductionmentioning

confidence: 99%

“…Trichinella infection in food animals is a serious threat to the meat food safety [6,7]. Therefore, it's necessary to develop anti-Trichinella vaccines to interrupt Trichinella infection in domestic pigs, and eliminate the ML in pork [8,9] After contaminated meat is eaten, T. spiralis ML are released from the capsules in stomach under the digestion of gastric juice, and activated into intestinal infectious larvae (IIL) after exposure to bile [10,11]. The IIL intrude into gut epithelium, and develop to adult worms (AW) after molting 4 times.…”

Section: Introductionmentioning

confidence: 99%

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Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice

et al. 2022

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Background Trichinella spiralis is a foodborne parasitic nematode which is a serious risk to meat safety. Development of anti-Trichinella vaccine is needed to control Trichinella infection in food animals. In this study, two novel T. spiralis genes (calreticulin and serine protease 1.1) in combination were used to construct oral DNA vaccines, and their induced protective immunity was evaluated in a murine model. Methodology/Principal findings TsCRT+TsSP1.1, TsCRT and TsSP1.1 DNA were transformed into attenuated Salmonella typhimurium ΔcyaSL1344. Oral vaccination of mice with TsCRT+TsSP1.1, TsCRT and TsSP1.1 DNA vaccines elicited a gut local mucosal sIgA response and systemic Th1/Th2 mixed response. Oral vaccination with TsCRT+TsSP1.1 induced obviously higher level of serum specific antibodies, mucosal sIgA and cellular immune response than either of single TsCRT or TsSP1.1 DNA vaccination. Oral vaccination of mice with TsCRT+TsSP1.1 exhibited a 53.4% reduction of enteral adult worms and a 46.05% reduction of muscle larvae, conferred a higher immune protection than either of individual TsCRT (44.28 and 42.46%) or TsSP1.1 DNA vaccine (35.43 and 29.29%) alone. Oral vaccination with TsCRT+TsSP1.1, TsCRT and TsSP1.1 also obviously ameliorated inflammation of intestinal mucosa and skeletal muscles of vaccinated mice after challenge. Conclusions TsCRT and TsSP1.1 might be regarded the novel potential targets for anti-Trichinella vaccines. Attenuated Salmonella-delivered DNA vaccine provided a prospective approach to control T. spiralis infection in food animals.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice

et al. 2022

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“…SCXK 2020–0004). These mice were bred in individual ventilated cages (IVC, Suzhou Fengshi Laboratory Animal Equipment Co., Ltd., Suzhou, China) [ 27 ]. Intestinal epithelium cells (IECs) were isolated from the small intestine of normal BALB/c mice, and negative control C2C12 cells were from mouse skeletal muscle myoblast cells that are insensitive to Trichinella larval penetration [ 28 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells

Song

Ning

et al. 2022

Vet Res

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The aim of this study was to investigate the characteristics of a novel type C lectin from Trichinella spiralis (TsCTL) and its role in larval invasion of intestinal epithelial cells (IECs). TsCTL has a carbohydrate recognition domain (CRD) of C-type lectin. The full-length TsCTL cDNA sequence was cloned and expressed in Escherichia coli BL21. The results of qPCR, Western blotting and immunofluorescence assays (IFAs) showed that TsCTL was a surface and secretory protein that was highly expressed at the T. spiralis intestinal infective larva (IIL) stages and primarily located at the cuticle, stichosome and embryos of the parasite. rTsCTL could specifically bind with IECs, and the binding site was localized in the IEC nucleus and cytoplasm. The IFA results showed that natural TsCTL was secreted and bound to the enteral epithelium at the intestinal stage of T. spiralis infection. The rTsCTL had a haemagglutinating effect on murine erythrocytes, while mannose was able to inhibit the rTsCTL agglutinating effect for mouse erythrocytes. rTsCTL accelerated larval intrusion into the IECs, whereas anti-rTsCTL antibodies and mannose significantly impeded larval intrusion in a dose-dependent manner. The results indicated that TsCTL specifically binds to IECs and promotes larval invasion of intestinal epithelium, and it might be a potential target of vaccines against T. spiralis enteral stages.

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Recombinant Toxoplasma gondii Calreticulin protein provides partial protection against acute and chronic toxoplasmosis

Zhang

Yang

et al. 2023

Acta Tropica

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Oral immunization with attenuated Salmonella encoding an elastase elicits protective immunity against Trichinella spiralis infection

Cited by 19 publications

References 83 publications

Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice

Oral vaccination of mice with attenuated Salmonella encoding Trichinella spiralis calreticulin and serine protease 1.1 confers protective immunity in BALB/c mice

A novel C-type lectin from Trichinella spiralis mediates larval invasion of host intestinal epithelial cells

Recombinant Toxoplasma gondii Calreticulin protein provides partial protection against acute and chronic toxoplasmosis

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