Recombinant Toxoplasma gondii Calreticulin protein provides partial protection against acute and chronic toxoplasmosis

Zhang, Jing; Yang, Zhenke; Ma, Hangbin; Tian, Xiaowei; Li, Jiani; Zhang, Zihang; Yang, Longxin; Feng, Mengchuang; Mei, Xuefang; Zhang, Zhenchao; Li, Xingrui; Wang, Shuai

doi:10.1016/j.actatropica.2023.106883

Cited by 2 publications

(2 citation statements)

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“…21 The severity of T. gondii infection largely depends on the individual's immune system; immunocompromised patients and foetuses face high infection rates, serious complications and even death. 22 Vaccination is one of the best ways to prevent diseases; hence, it is imperative to develop candidate vaccines for toxoplasmosis. 23 Protein vaccines can induce a strong humoral response by inducing antigen-specific antibodies.…”

Section: Discussionmentioning

confidence: 99%

“…Toxoplasmosis is one of the most dangerous zoonotic diseases, causing serious economic losses worldwide due to abortion and reproductive problems 21 . The severity of T. gondii infection largely depends on the individual's immune system; immunocompromised patients and foetuses face high infection rates, serious complications and even death 22 . Vaccination is one of the best ways to prevent diseases; hence, it is imperative to develop candidate vaccines for toxoplasmosis 23 .…”

Section: Discussionmentioning

confidence: 99%

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Comparison of the immune response and protection against the experimental Toxoplasma gondii infection elicited by immunization with the recombinant proteins BAG1, ROP8, and BAG1‐ROP8

Xing,

Yang,

Yao

et al. 2024

Parasite Immunology

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Toxoplasmosis is one of the most dangerous zoonotic diseases, causing serious economic losses worldwide due to abortion and reproductive problems. Vaccination is the best way to prevent disease; thus, it is imperative to develop a candidate vaccine for toxoplasmosis. BAG1 and ROP8 have the potential to become vaccine candidates. In this study, rTgBAG1, rTgROP8, and rTgBAG1‐rTgROP8 were used to evaluate the immune effect of vaccines in each group by detecting the humoral and cellular immune response levels of BABL/c mice after immunization and the ability to resist acute and chronic infection with Toxoplasma gondii (T. gondii). We divided the mice into vaccine groups with different proteins, and the mice were immunized on days 0, 14, and 28. The protective effects of different proteins against T. gondii were analysed by measuring the cytokines, serum antibodies, splenocyte proliferation assay results, survival time, and number and diameter of brain cysts of mice after infection. The vaccine groups exhibited substantially higher IgG, IgG1, and IgG2a levels and effectively stimulated lymphocyte proliferation. The levels of IFN‐γ and IL‐2 in the vaccine group were significantly increased. The survival time of the mice in each vaccine group was prolonged and the diameter of the cysts in the vaccine group was smaller; rTgBAG1‐rTgROP8 had a better protection. Our study showed that the rTgBAG1, rTgROP8, and rTgBAG1‐rTgROP8 recombinant protein vaccines are partial but effective approaches against acute or chronic T. gondii infection. They are potential candidates for a toxoplasmosis vaccine.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%