Organ-specific expression of different histone H3 and H4 gene subfamilies in developing and adult maize

Chaubet, Nicole; Clément, Bernadette; Philipps, Gabriel; Gigot, Claude

doi:10.1007/bf00037077

Cited by 26 publications

(15 citation statements)

References 13 publications

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“…These results suggest that the expression of HMG2 could be restricted to tissues containing actively dividing cells. This assumption is supported by our results on the pattern of accumulation of HMG2 mRNA, which closely resembles that of histone H4 mRNA, which is known to be preferentially expressed in young tissues with high mitotic activity (37). While the broad expression of HMG1 suggests that it may encode a housekeeping form of HMGR, the enzyme encoded by HMG2 might have a more specialized role in the synthesis of specific isoprenoids required in actively dividing cells.…”

Section: Discussionsupporting

confidence: 78%

“…It is worth noting that the levels of HMG1 mRNA are much higher than those of HMG2 mRNA, which was detected only when poly(A)+ RNA was used. Since HMG2 mRNA was exclusively detected in parts of the plant containing meristematic tissues, the same mRNA samples were analyzed for the presence of histone H4 mRNA, known to be expressed in actively dividing cells (37). Fig.…”

Section: Materlils and Methodsmentioning

confidence: 99%

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Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, which encode microsomal forms of the enzyme.

Enjuto

Balcells

Campos

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

179

157

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The enzyme 3-hydroxy-3-methylglutarylCoA reductase (HMGR; EC 1.1.1.34) catalyzes the first ratelimiting step in plant isoprenoid biosynthesis. Arabidopsis thaliana contains two genes, HMGI and HMG2, that encode HMGR. We have doned these two genes and analyzed their structure and expression. HMGI and HMG2 consist of four exons and three small introns that interrupt the coding sequence at equivalent positions. The two genes share sequence similarity in the coding regions but not in the 5'-or 3'-flanking regions. HMG1 mRNA is detected in all tissues, whereas the presence of HMG2 mRNA is restricted to young seedlings, roots, and inflorescences. The similarity between the two encoded proteins (HMGR1 and HMGR2) is restricted to the regions corresponding to the membrane and the catalytic domains. Arabidopsis HMGR2 represents a divergent form of the enzyme that has no counterpart among plant HMGRs characterized so far. By using a coupled in vitro transcriptiontranslation assay, we show that both HMGR1 and HMGR2 are cotranslationally inserted into endoplasmic reticulum-derived microsomal membranes. Our results suggest that the endoplasmic reticulum is the only cell compartment for the targeting of HMGR in Arabidopsis and support the hypothesis that in higher plants the formation of mevalonate occurs solely in the cytosol.

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Section: Discussionsupporting

confidence: 78%

Section: Materlils and Methodsmentioning

confidence: 99%

Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, which encode microsomal forms of the enzyme.

Enjuto

Balcells

Campos

et al. 1994

Proc. Natl. Acad. Sci. U.S.A.

179

157

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“…Northern analysis in various tissues of plants indicated that they are actively transcribed in tissues with high mitotic activity, suggesting that most of plant histone genes primarily belong to a class of replication-dependent histone genes [4,11,18,25]. However, these studies also demonstrated that histone mRNAs are detected at low levels in tissues that do not have division activities, suggesting that higher plants also carry replication-independent histone genes.…”

Section: Introductionmentioning

confidence: 73%

A type I element composed of the hexamer (ACGTCA) and octamer (CGCGGATC) motifs plays a role(s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants

Terada¹,

Nakayama

Iwabuchi

et al. 1995

Plant Mol Biol

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Type I element (CCACGTCACCGATCCGCG) is a well-conserved regulatory element found in proximal promoter region of a certain class of plant histone genes, that is composed of two independent cis-acting elements of the hexamer (ACGTCA) and the reverse-oriented octamer (GATCCGCG) motifs. To investigate functional role(s) of the type I element in regulation of a wheat histone H3 gene (TH012) promoter activity in vivo, base substitution mutations were introduced into the element and activities of the mutated promoters were examined in cultured rice cells, and in regenerated roots and anther walls of transgenic rice plants by employing a GUS reporter system. Mutations of each or both of the hexamer and the octamer motifs caused a reduction in the promoter activity in protoplasts transfected transiently or stably transformed calli. The mutation of the octamer motif with or without the mutation of the hexamer motif caused a marked reduction of the promoter activity in the root meristem of transgenic rice although the mutation of the hexamer motif alone caused a weak reduction. In contrast to these results, no effect of the mutations of either the hexamer or the octamer motif was found in the anther wall in which replication-independent activity of the H3 promoter was observed. Our results suggested that the hexamer and the octamer motifs may play important role(s) in regulation of replication-dependent but not of replication-independent expression of the wheat histone H3 gene.

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“…Chromatin structure and sensitivity to nucleases of the histone H3C4 gene promoter region Expression of the genes encoding histones progressively increases during the early steps of maize germination to reach a maximum after 4 days [5]. At that stage the concentration of histone mRNA in embryos is high, although they are present primarily in meristematic tissues, i.e.…”

Section: Resultsmentioning

confidence: 99%

“…The H3C4 gene which is studied here is a member of a small multigene subfamily of 4-5 copies. Its expression as well as that of the histone genes in general is under a strict regulatory control resulting in a preferential accumulation of specific mRNAs in proliferating tissues [5,13]. Evaluation of the activity of an Arabidopsis H4 promoter in transgenic plants strongly suggests the regulation to occur essentially at the level ofgene transcription [ 1 ], and the expression to result from both a low replication-independent and an enhanced replication-dependent activity [151.…”

Section: Introductionmentioning

confidence: 99%

Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter

et al. 1993

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A 1 kb region of a maize H3 histone gene promoter has been analysed at a structural and functional level. Micrococcal nuclease digestion of isolated nuclei showed that the promoter region is organized into nucleosomes but a zone extending over approximately one nucleosome (20 to 230 bp upstream of the TATA box) displays remarkable accessibility to digestion. Three DNase I-hypersensitive sites were found within this zone at the vicinity of consensus sequences, some of which are already known to act as cis elements. This promoter region is able to direct faithful expression of the GUS reporter gene in meristematic tissues of transgenic tobacco plants.

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Organ-specific expression of different histone H3 and H4 gene subfamilies in developing and adult maize

Cited by 26 publications

References 13 publications

Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, which encode microsomal forms of the enzyme.

Arabidopsis thaliana contains two differentially expressed 3-hydroxy-3-methylglutaryl-CoA reductase genes, which encode microsomal forms of the enzyme.

A type I element composed of the hexamer (ACGTCA) and octamer (CGCGGATC) motifs plays a role(s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants

Nuclease sensitivity and functional analysis of a maize histone H3 gene promoter

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