Organic–inorganic nanovesicles for doxorubicin storage and release

Leung, Siu Ling; Zha, Zhengbao; Teng, Weibing; Cohn, Celine; Dai, Zhifei; Wu, Xiaoyi

doi:10.1039/c2sm07452d

Cited by 29 publications

(20 citation statements)

References 37 publications

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“…Quenching of fluorescence of DOX after its loading into the membrane of DNA‐PE vesicle can be attributed to the self‐quenching of fluorescence due to the loading of DOX into the membrane of the vesicle as reported in similar systems . Drug loading efficiency of DNA‐PE vesicle is found to be 9.5 %, which is comparable with the similar reported systems …”

Section: Resultssupporting

confidence: 85%

DNA‐Decorated Luminescent Vesicles as Drug Carriers

et al. 2016

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DNA based spherical nanostructures is one of the promising nanocarriers for drug delivery applications because of their excellent biocompatibility and unique structural features of DNA. Herein, we report the amphiphilicity driven self-assembly of DNA-phenyleneethynylene based amphiphile into luminescent vesicles and their cellular uptake toward HeLa cell. Spectroscopic and microscopic analyses reveal the self-assembly of this class of amphiphiles into vesicular nanostructures with corona made of DNA and a functional hydrophobic membrane. Vesicles exhibit promising cell permeability.

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Section: Resultssupporting

confidence: 85%

DNA‐Decorated Luminescent Vesicles as Drug Carriers

et al. 2016

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“…Doxorubicin was added at a concentration of 1 μM. At times 0, 4, 8, 16, and 24 min after the addition of doxorubicin the cells were washed three times with PBS, resuspended in 500 μL of 50% MeOH/H 2 O, sonicated 5 × 10 s, centrifuged 10 min at 5000 g, and the supernatant was analysed by fluorimetry with a fluorimeter CytoFluor® series 4000 multiwell plate reader, using the 485/20 filter for excitation and 590/ 30 filter for emission, in agreement to the fluorescence characteristics of doxorubicin [27]. The extraction step was done to avoid artefacts due to cell components such as proteins and DNA, and methanol was chosen due to the high recovery of doxorubicin previously reported [28].…”

Section: Fluorescence Measurement Of Intracellular Doxorubicin Concenmentioning

confidence: 99%

In situ Fourier transform infrared analysis of live cells' response to doxorubicin

Fale

Altharawi

Chan

2015

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research

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The study of the response of cancer cells to chemotherapy drugs is of high importance due to the specificity of some drugs to certain types of cancer and the resistance of some specific cancer types to chemotherapy drugs. Our aim was to develop and apply the label-free and non-destructive Fourier transform infrared (FTIR) method to determine the sensitivity of three different cancer cell-lines to a common anti-cancer drug doxorubicin at different concentrations and to demonstrate that information about the mechanism of resistance to the chemotherapy drug can be extracted from spectral data. HeLa, PC3, and Caco-2 cells were seeded and grown on an attenuated total reflection (ATR) crystal, doxorubicin was applied at the clinically significant concentration of 0.1-20 μM, and spectra of the cells were collected hourly over 20 h. Analysis of the amide bands was correlated with cell viability, which had been cross validated with MTT assays, allowing to determine that the three cell lines had significantly different resistance to doxorubicin. The difference spectra and principal component analysis (PCA) highlighted the subtle chemical changes in the living cells under treatment. Spectral regions assigned to nucleic acids (mainly 1085 cm(-1)) and carbohydrates (mainly 1024 cm(-1)) showed changes that could be related to the mode of action of the drug and the mechanism of resistance of the cell lines to doxorubicin. This is a cost-effective method that does not require bioassay reagents but allows label-free, non-destructive and in situ analysis of chemical changes in live cells, using standard FTIR equipment adapted to ATR measurements.

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“…In general, small molecules are placed in a groove binding due to geometrical compatibility with specific base sequences and are intercalated with nucleobases through hydrogen bonding and π-π stacking 10 . Doxorubicin hydrochloride (DOX) is a well-known anthraquinone anticancer drug molecule that is extensively used in anthracycline, and it has been proven effective against various human malignancies, including various types of cancers 11 12 13 14 .…”

mentioning

confidence: 99%

Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

Gnapareddy

Dugasani

et al. 2015

Sci Rep

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Double-stranded salmon DNA (SDNA) was doped with doxorubicin hydrochloride drug molecules (DOX) to determine the binding between DOX and SDNA, and DOX optimum doping concentration in SDNA. SDNA thin films were prepared with various concentrations of DOX by drop-casting on oxygen plasma treated glass and quartz substrates. Fourier transform infrared (FTIR) spectroscopy was employed to investigate the binding sites for DOX in SDNA, and electrical and photoluminescence (PL) analyses were used to determine the optimum doping concentration of DOX. The FTIR spectra showed that up to a concentration of 30 μM of DOX, there was a tendency for binding with a periodic orientation via intercalation between nucleosides. The current and PL intensity increased as the DOX concentration increased up to 30 μM, and then as the concentration of DOX further increased, we observed a decrease in current as well as PL quenching. Finally, the optical band gap and second band onset of the transmittance spectra were analyzed to further verify the DOX binding and optimum doping concentration into SDNA thin films as a function of the DOX concentration.

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Organic–inorganic nanovesicles for doxorubicin storage and release

Cited by 29 publications

References 37 publications

DNA‐Decorated Luminescent Vesicles as Drug Carriers

DNA‐Decorated Luminescent Vesicles as Drug Carriers

In situ Fourier transform infrared analysis of live cells' response to doxorubicin

Chemical and Physical Characteristics of Doxorubicin Hydrochloride Drug-Doped Salmon DNA Thin Films

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