Organization and evolution of a higher plant alphoid-like satellite DNA sequence

Grellet, Françoise; Delcasso, Dominique; Panabières, Franck; Delseny, Michel

doi:10.1016/0022-2836(86)90329-3

Cited by 111 publications

(51 citation statements)

References 46 publications

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“…Southem Blot Analysis DNA was extracted from frozen leaves and purified on an ethidium bromide CsCl gradient, as previously reported (9). Four micrograms of DNA was digested with an excess of restriction enzymes according to the supplier's recommendations.…”

Section: Dna Sequencingmentioning

confidence: 99%

Characterization of a Radish Nuclear Gene Expressed during Late Seed Maturation

Raynal¹,

Depigny²,

Cooke³

et al. 1989

Plant Physiol.

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To study long-lived mRNAs stored in radish (Raphanus sativus) seed, we have selected clones from a dry seed cDNA library by differential screening. One of these clones, p8B6, whose mRNAs are abundant in the dry seed, was characterized. This clone hybridizes to an RNA class of approximately 600 nucleotides whose accumulation begins during the desiccation phase, reaches its maximum level in the dry seed, and is no longer detectable in 12 hour old seedlings. mRNAs hybrid-selected by p8B6 encode four polypeptides, but only two are compatible with the size class of RNAs detected by Northem analysis. Three of them have previously been identified as major 'early germination' polypeptides, and their synthesis has been shown to be induced prematurely in immature embryos by a desiccation treatment. The protein deduced from the p8B6 nucleotide sequence is 9 kilodaltons in size, highly hydrophilic, rich in Gly and Glu, and contains no Cys, Trp, and lie. The amino acid sequence shares good homology with that of two recently described seed proteins: a cotton late embryogenesis abundant protein and the wheat early methionine-labeled protein. Southem blot analysis suggests that the p8B6 sequence belongs to a very small gene family. The exact function of the product encoded by p8B6 remains to be determined.Dry seed embryos contain translatable stored mRNAs, which encode the first proteins synthesized during imbibition (1,3,22,25). However, the nature, origin, and function of the proteins encoded by these long-lived mRNAs remain unclear. Do they correspond to embryogenesis-specific polypeptides or do they play an essential role during early germination? To answer this question, several groups (1,24,25) have analyzed proteins and mRNA at different stages of development. Proteins were analyzed by staining, while the mRNA population was studied by radioactive labeling of proteins in vivo or by an in vitro translation system. Up to now, several major gene families have been distinguished by their differential expression (1,4,23,24

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Section: Dna Sequencingmentioning

confidence: 99%

Characterization of a Radish Nuclear Gene Expressed during Late Seed Maturation

Raynal¹,

Depigny²,

Cooke³

et al. 1989

Plant Physiol.

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“…The other prominent class of dispersed repetitive sequences is also widely distributed in many plant taxa (Guidet et al, 1991;Heuros et al, 1993;Kubis et al, 1998;Katsiotis et al, 2000;Linares et al, 2000;Ananiev et al, 2002). Tandemly repeated DNA sequences have been variously classi- 175-180 Capesius, 1983;Benslimane et al, 1986;Grellet et al, 1986;Lakshmikumaran and Ranade, 1990;Xia et al, 1993;Harrison and Heslop-Harrison, 1995 …”

confidence: 99%

“…For example, the 170-180-bp related centromeric satellite sequences, widely distributed in several Brassica species and other Cruciferae genera (with 75-100% sequence homology) (Grellet et al, 1986;Xia et al, 1993;Harrison and Heslop-Harrison, 1995;Heslop-Harrison et al, 2003), exhibit homology with several tRNA genes thereby suggesting their origin from short sequences related to tRNA genes and SINEs (short interspersed nuclear elements homologous to tRNAs) (Benslimane et al, 1986;Harrison and Heslop-Harrison, 1995). The 177-bp monomeric unit of the above type, composed of three extensively diverged internal repeats of F60 bp, appears to have evolved from an ancestral block of 60 bp made up of two 30-bp distinct elements (Grellet et al, 1986;Xia et al, 1993).…”

Section: Satellite Dna Families "Derivative" Of Known Class Of Sequencesmentioning

confidence: 99%

Organization and evolution of highly repeated satellite DNA sequences in plant chromosomes

Sharma

Raina²

2005

Cytogenet Genome Res

108

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A major component of the plant nuclear genome is constituted by different classes of repetitive DNA sequences. The structural, functional and evolutionary aspects of the satellite repetitive DNA families, and their organization in the chromosomes is reviewed. The tandem satellite DNA sequences exhibit characteristic chromosomal locations, usually at subtelomeric and centromeric regions. The repetitive DNA family(ies) may be widely distributed in a taxonomic family or a genus, or may be specific for a species, genome or even a chromosome. They may acquire large-scale variations in their sequence and copy number over an evolutionary time-scale. These features have formed the basis of extensive utilization of repetitive sequences for taxonomic and phylogenetic studies. Hybrid polyploids have especially proven to be excellent models for studying the evolution of repetitive DNA sequences. Recent studies explicitly show that some repetitive DNA families localized at the telomeres and centromeres have acquired important structural and functional significance. The repetitive elements are under different evolutionary constraints as compared to the genes. Satellite DNA families are thought to arise de novo as a consequence of molecular mechanisms such as unequal crossing over, rolling circle amplification, replication slippage and mutation that constitute “molecular drive”.

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“…Alphoid-like satellite DNA sequences have been characterised for a number of plant species including radish [31], cereals [9], and members of the Liliaceae [32]. In all cases these sequences were located in the variable heterochromatic regions of specific chromosomes.…”

Section: Cytological Analysismentioning

confidence: 99%

Chromosomal localization of a tandemly repeated DNA sequence in Trifolium repens L.

Ellison

Rowland

1996

Cell Res

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A karyotype of Trifolium repens constructed from mitotic cells revealed 13 pairs of metacentric and 3 pairs of submetacentric chromosomes including a pair of satellites located at the end of the short arm of chromosome 16. C-bands were identified around the centromeric regions of 8 pairs of chromosomes. A 350 bp tandemly repeated DNA sequence from T. repens labelled with digoxygenin hybridized to the proximal centromeric regions of 12 chromosome pairs. Some correlation between the distribution of the repeat sequence and the distribution of C-banding was demonstrated.

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Organization and evolution of a higher plant alphoid-like satellite DNA sequence

Cited by 111 publications

References 46 publications

Characterization of a Radish Nuclear Gene Expressed during Late Seed Maturation

Characterization of a Radish Nuclear Gene Expressed during Late Seed Maturation

Organization and evolution of highly repeated satellite DNA sequences in plant chromosomes

Chromosomal localization of a tandemly repeated DNA sequence in Trifolium repens L.

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