Organization and structure of NADH-dependent glutamate synthase gene from rice plants

Goto, Satoshi; Akagawa, Takumi; Kojima, Shinichi; Hayakawa, Toshihiko; Yamaya, Tomoyuki

doi:10.1016/s0167-4838(98)00142-3

Cited by 52 publications

(32 citation statements)

References 38 publications

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“…Radiotracer studies suggested that indica rice utilizes nitrate better than ammonia, the predominant form of N in flooded fields fertilized with urea (Kronzucker et al, 2000). The enzymes and genes of Nmetabolism in rice are known (Choi et al, 1989;Sakamoto et al, 1989;Goto et al, 1998) and their regulation by N metabolites and signaling processes have also begun to be understood (Ali et al, 2007a,b).…”

Section: Introductionmentioning

confidence: 99%

Spirulina nitrate-assimilating enzymes (NR, NiR, GS) have higher specific activities and are more stable than those of rice

Ali

Jha

Sandhu

et al. 2008

Physiol Mol Biol Plants

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Spirulina platensis, a cyanobacterium whose N-metabolic pathway is similar to that of higher plants like rice (Oryza sativa), produces tenfold more protein, indicating a higher capacity for nitrate utilization/removal. Our in vitro analyses in crude extracts revealed that this can be attributed, at least in part, to the higher specific activities (3-6 fold) and half lives (1.2-4.4 fold) of the N-assimilating enzymes, nitrate reductase (NR), nitrite reductase (NiR) and glutamine synthetase (GS) in

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Section: Introductionmentioning

confidence: 99%

Spirulina nitrate-assimilating enzymes (NR, NiR, GS) have higher specific activities and are more stable than those of rice

Ali

Jha

Sandhu

et al. 2008

Physiol Mol Biol Plants

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“…A full-length cDNA for OsAMT3;1 was also amplified by RT-PCR against rice-root poly(A) + RNA using information of a rice genome sequence (Rice Genome Project). Sequencing, as well as gel blot analyses of DNA and RNA, was carried out as described previously (Goto et al 1998). To exclude PCR errors, all PCR products were produced in dupli- Fig.…”

mentioning

confidence: 99%

“…The following primers were used: 5¢-TCTCACCGAACAACATCCTGCT-3¢ as forward primer and 5¢-TTGCCGATCTGCTTGATACC-3¢ as reverse primer for OsAMT2;1; 5¢-GTCCCGCTCTGGCTCACCTT-3¢ as forward primer and 5¢-CGGGGAGGAAGAGGGAGCA-3¢ as reverse primer for OsAMT3;1; 5¢-CCTTTTGCTTGCCATGGTATT-3¢ as forward primer and 5¢-AAGCCCATATATCAACCAGCA-3¢ as reverse primer for GAPDH. Specificity of each primer was confirmed by PCR using a rice-root cDNA library (Goto et al 1998) as a template.…”

mentioning

confidence: 99%

Constitutive Expression of a Novel-Type Ammonium Transporter OsAMT2 in Rice Plants

Suenaga¹,

Moriya²,

Sonoda³

et al. 2003

Plant and Cell Physiology

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175

105

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;To characterize ammonium transport pathways in rice, two cDNAs with high homology to MEP/AMT2-type ammonium transporters, OsAMT2;1 and OsAMT3;1, were isolated. Expression of OsAMT2;1 in an ammoniumuptake-defective yeast mutant showed that this gene encodes functional ammonium transporters. OsAMT2;1 was constitutively expressed in both roots and shoots irrespective of the supply of inorganic nitrogen to the medium, whereas OsAMT3;1 expression was relatively weak. A database search with the amino acid sequence of OsAMT2;1 showed that there are 10 putative OsAMT genes in rice, i.e. three each for OsAMT1, OsAMT2 and OsAMT3, respectively, and one for OsAMT4.

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“…Metabolic profiles also changed in transgenic potato differentially expressing the external mitochondrial NADPH dehydrogenase [47]. NADH-GOGAT1 is predicted to be present in the plastids [3]; the altered content of NAD(H) affects the TCA cycle enzymes through translocation of substrates such as 2-OG and citrate between the cytosol and organelles [45,48]. As reported previously, repression of mitochondrial enzymes results in changes to metabolic profiles [49].…”

Section: Nadh-gogat and Pyridine Nucleotides Statusmentioning

confidence: 54%

“…The major physiological role of Fd-GOGAT was suggested to be re-assimilation of the ammonium liber-ated in photorespiration [2]. cDNA clones of NADH-GOGAT have been isolated from several plants such as alfalfa (Medicago sativa), Arabidopsis thaliana, rice [3], and soybean (Glycine max) [4]. Fd-GOGAT cDNAs have been cloned from maize (Zea mays), tobacco (Nicotiana tabacum), barley (Hordeum vulgare), spinach (Spinacia oleracea), Scotch pine (Pinus sylvestris), and Arabidopsis [2].…”

mentioning

confidence: 99%

Suppression of glutamate synthase genes significantly affects carbon and nitrogen metabolism in rice (Oryza sativa L.)

Luo

Yang

et al. 2011

Sci. China Life Sci.

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Rice (Oryza sativa) glutamate synthase (GOGAT, EC 1.4.1.14) enzymes have been proposed to have great potential for improving nitrogen use efficiency, but their functions in vivo and their effects on carbon and nitrogen metabolism have not been systematically explored. In this research, we analyzed transcriptional profiles of rice GOGAT genes using a genome-wide microarray database, and investigated the effects of suppression of glutamate synthase genes on carbon and nitrogen metabolism using GOGAT co-suppressed rice plants. Transcriptional profiles showed that rice GOGAT genes were expressed differently in various tissues and organs, which suggested that they have different roles in vivo. Compared with the wild-type, tiller number, total shoot dry weight, and yield of GOGAT co-suppressed plants were significantly decreased. Physiological and biochemical studies showed that the contents of nitrate, several kinds of free amino acids, chlorophyll, sugars, sugar phosphates, and pyridine nucleotides were significantly decreased in leaves of GOGAT co-suppressed plants, but the contents of free ammonium, 2-oxoglutarate, and isocitrate in leaves were increased. We conclude that GOGATs play essential roles in carbon and nitrogen metabolism, and that they are indispensable for efficient nitrogen assimilation in rice.glutamate synthase, Oryza sativa, co-suppression, carbon and nitrogen metabolism Citation:Lu Y E, Luo F, Yang M, et al. Suppression of glutamate synthase genes significantly affects carbon and nitrogen metabolism in rice (Oryza sativa

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Organization and structure of NADH-dependent glutamate synthase gene from rice plants

Cited by 52 publications

References 38 publications

Spirulina nitrate-assimilating enzymes (NR, NiR, GS) have higher specific activities and are more stable than those of rice

Spirulina nitrate-assimilating enzymes (NR, NiR, GS) have higher specific activities and are more stable than those of rice

Constitutive Expression of a Novel-Type Ammonium Transporter OsAMT2 in Rice Plants

Suppression of glutamate synthase genes significantly affects carbon and nitrogen metabolism in rice (Oryza sativa L.)

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