Organization of the capsule biosynthesis gene locus of the oral streptococcus Streptococcus anginosus

Tsunashima, Hiroyuki; Miyake, Katsuhide; Motono, Makoto; Iijima, Shinji

doi:10.1016/j.jbiosc.2011.10.013

Cited by 12 publications

(15 citation statements)

References 31 publications

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“…1995; Yang et al. 2009) and the S. anginosus CPS locus was first characterized in strain ATCC 33397 (Tsunashima et al. 2012).…”

Section: Resultsmentioning

confidence: 99%

“…3). In figure 3, strain ATCC 33397 was used as a representative genome containing CPS locus (Tsunashima et al. 2012).…”

Section: Resultsmentioning

confidence: 99%

“…3.—The capsular polysaccharide (CPS) gene clusters from Streptococcus anginosus strains ATCC 33397, J4211, and J4206. The CPS region of S. anginosus was first described in strain ATCC 33397 (Tsunashima et al 2012), which was used as a baseline for comparison to the same cluster in strains J4211 and J4206. The J4206 CPS gene cluster shown in the figure is the region that includes genes SanJ4206_1756 through SanJ4206_1786 on the genome.…”

Section: Resultsmentioning

confidence: 99%

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Comparative Genome Analysis of the Daptomycin-ResistantStreptococcus anginosusStrain J4206 Associated with Breakthrough Bacteremia

et al. 2016

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Streptococcus anginosus is a member of the normal oral flora that can become a pathogen causing pyogenic infections in humans. The genome of daptomycin-resistant strain J4206, originally isolated from a patient suffering from breakthrough bacteremia and septic shock at the University of Texas Health Science Center at San Antonio, was determined. The circular genome is 2,001,352 bp long with a GC content of 38.62% and contains multiple mobile genetic elements, including the phage-like chromosomal island SanCI that mediates a mutator phenotype, transposons, and integrative conjugative elements. Daptomycin resistance involves multiple alterations in the cell membrane and cell wall, and unique features were identified in J4206 that may contribute to resistance. A cluster of capsular polysaccharide (CPS) genes for choline metabolism and transport are present that may help neutralize cell surface charges, destabilizing daptomycin binding. Further, unique J4206 genes encoding sortases and LPXTG-target proteins that are involved in cell wall modification were present. The J4206 genome is phylogenetically closely related to the recently reported vancomycin-resistant SA1 strain; however, these genomes differ with SNPs in cardiolipin synthetase, histidine kinase yycG, teichoic acid modification genes, and other genes involved in cell surface modification. Transmission electron microscopy showed that the cell walls of both strains J4206 and SA1 were significantly thicker and more electron dense than daptomycin- and vancomycin-sensitive strain J4211. This comparative genomic study has identified unique genes as well as allelic variants in the J4206 genome that are involved in cell surface modification and thus might contribute to the acquisition of daptomycin resistance.

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“…1995; Yang et al. 2009) and the S. anginosus CPS locus was first characterized in strain ATCC 33397 (Tsunashima et al. 2012).…”

Section: Resultsmentioning

confidence: 99%

“…3). In figure 3, strain ATCC 33397 was used as a representative genome containing CPS locus (Tsunashima et al. 2012).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Genome Analysis of the Daptomycin-ResistantStreptococcus anginosusStrain J4206 Associated with Breakthrough Bacteremia

et al. 2016

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“…Employing our knowledge about competence kinetics in S. anginosus , we report a Cre‐recombinase‐based mutation technique that allows fast and efficient construction of gene deletions in this species. Gene deletions in the SAG are currently performed through allelic exchange and the integration of an antibiotic resistance marker . These methods are highly selective but the generation of multiple gene deletions in the same strain can be limited by the availability of different resistance markers.…”

Section: Discussionmentioning

confidence: 99%

“…Gene deletions in the SAG are currently performed through allelic exchange and the integration of an antibiotic resistance marker. 36,47,48 These methods are highly selective but the generation of multiple gene deletions in the same strain can be limited by the availability of different resistance markers. In a second transformation step the Cre-recombinase encoded on pAT18-cre-rec tufA is introduced into the mutant leading to the desired excision of spc.…”

Section: Discussionmentioning

confidence: 99%

The competence system of Streptococcus anginosus and its use for genetic engineering

Bauer

Mauerer

Grempels

et al. 2018

Molecular Oral Microbiology

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Streptococcus anginosus is considered a human commensal but improvements in species identification in recent years have highlighted its role as an emerging pathogen. However, our knowledge about the pathogenicity mechanisms in this species is scarce. One reason for this is the lack of published genetic manipulation techniques in the S. anginosus group. To establish a novel mutation technique we investigated the competence system of S. anginosus and created a Cre-recombinase-based mutation method that allows the generation of markerless gene deletions in S. anginosus. In silico analysis of the competence system demonstrated that S. anginosus encodes homologues for the vast majority of genes that are known to be essential for the transformation of S. pneumoniae. Analysis of transformation kinetics confirmed that S. anginosus SK52 possesses an S. pneumoniae-like competence development with a rapid increase of competence after treatment with Competence Stimulating Peptide (CSP), reaching a maximum transformation efficiency of 0.24% ± 0.08%. The combination of CSP-induced transformation and the Cre-lox system allows the efficient and fast creation of markerless gene deletions and will facilitate the investigation of the pathogenicity of S. anginosus on a genetic level.

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Bicarbonate and unsaturated fatty acids enhance capsular polysaccharide synthesis gene expression in oral streptococci, Streptococcus anginosus

Matsumoto

Miyake

Ozawa

et al. 2019

Journal of Bioscience and Bioengineering

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Organization of the capsule biosynthesis gene locus of the oral streptococcus Streptococcus anginosus

Cited by 12 publications

References 31 publications

Comparative Genome Analysis of the Daptomycin-ResistantStreptococcus anginosusStrain J4206 Associated with Breakthrough Bacteremia

Comparative Genome Analysis of the Daptomycin-ResistantStreptococcus anginosusStrain J4206 Associated with Breakthrough Bacteremia

The competence system of Streptococcus anginosus and its use for genetic engineering

Bicarbonate and unsaturated fatty acids enhance capsular polysaccharide synthesis gene expression in oral streptococci, Streptococcus anginosus

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