Organization of the Catecholaminergic System in the Short-Lived Fish Nothobranchius furzeri

Borgonovo, Janina; Ahumada-Galleguillos, Patricio; Oñate-Ponce, Alejandro; Allende-Castro, Camilo; Henny, Pablo; Concha, Miguel L.

doi:10.3389/fnana.2021.728720

Cited by 8 publications

(2 citation statements)

References 98 publications

(158 reference statements)

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“…In contrast, midbrain DA cells have been identified in the tegmental area of cartilaginous fish (Carrera et al, 2005 , 2012 ). Recently, several studies have reported Th-expressing neurons in different actinopterygian species in similar brain regions compared with the neurons we identified in the tegmental part of the midbrain (Lopez et al, 2019 ; Lozano et al, 2019 ; Borgonovo et al, 2021 ). This suggests that our newly identified Th-expressing neurons are not an exclusive feature of zebrafish but may be evolutionary remnants of the midbrain DA neurons found in cartilaginous fish, which are lost or severely reduced in most teleost species.…”

Section: Discussionsupporting

confidence: 74%

CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain

Altbürger,

Holzhauser,

Driever

2023

Front. Neuroanat.

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Catecholaminergic neuron clusters are among the most conserved neuromodulatory systems in vertebrates, yet some clusters show significant evolutionary dynamics. Because of their disease relevance, special attention has been paid to mammalian midbrain dopaminergic systems, which have important functions in motor control, reward, motivation, and cognitive function. In contrast, midbrain dopaminergic neurons in teleosts were thought to be lost secondarily. Here, we generated a CRISPR/Cas9-based knock-in transgene at the th locus, which allows the expression of the Q-system transcription factor QF2 linked to the Tyrosine hydroxylase open reading frame by an E2A peptide. The QF2 knock-in allele still expresses Tyrosine hydroxylase in catecholaminergic neurons. Coexpression analysis of QF2 driven expression of QUAS fluorescent reporter transgenes and of th mRNA and Th protein revealed that essentially all reporter expressing cells also express Th/th. We also observed a small group of previously unidentified cells expressing the reporter gene in the midbrain and a larger group close to the midbrain–hindbrain boundary. However, we detected no expression of the catecholaminergic markers ddc, slc6a3, or dbh in these neurons, suggesting that they are not actively transmitting catecholamines. The identified neurons in the midbrain are located in a GABAergic territory. A coexpression analysis with anatomical markers revealed that Th-expressing neurons in the midbrain are located in the tegmentum and those close to the midbrain–hindbrain boundary are located in the hindbrain. Our data suggest that zebrafish may still have some evolutionary remnants of midbrain dopaminergic neurons.

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Section: Discussionsupporting

confidence: 74%

CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain

Altbürger,

Holzhauser,

Driever

2023

Front. Neuroanat.

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“…Since the overall composition of dopaminergic neurons is conserved among teleosts, the distribution of dopaminergic neurons in turquoise killifish is similar to zebrafish and medaka. Please refer to the latest report on the analysis of catecholaminergic neurons in the central nervous system of turquoise killifish [12].…”

Section: Central Nervous System In Small Fishesmentioning

confidence: 99%

Zebrafish, Medaka and Turquoise Killifish for Understanding Human Neurodegenerative/Neurodevelopmental Disorders

Kodera

Matsui

2022

IJMS

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In recent years, small fishes such as zebrafish and medaka have been widely recognized as model animals. They have high homology in genetics and tissue structure with humans and unique features that mammalian model animals do not have, such as transparency of embryos and larvae, a small body size and ease of experiments, including genetic manipulation. Zebrafish and medaka have been used extensively in the field of neurology, especially to unveil the mechanisms of neurodegenerative diseases such as Parkinson’s and Alzheimer’s disease, and recently, these fishes have also been utilized to understand neurodevelopmental disorders such as autism spectrum disorder. The turquoise killifish has emerged as a new and unique model animal, especially for ageing research due to its unique life cycle, and this fish also seems to be useful for age-related neurological diseases. These small fishes are excellent animal models for the analysis of human neurological disorders and are expected to play increasing roles in this field. Here, we introduce various applications of these model fishes to improve our understanding of human neurological disorders.

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Immunohistochemical characterisation of the adult Nothobranchius furzeri intestine

Borgonovo,

Allende-Castro,

Medinas

et al. 2023

Cell Tissue Res

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Organization of the Catecholaminergic System in the Short-Lived Fish Nothobranchius furzeri

Cited by 8 publications

References 98 publications

CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain

CRISPR/Cas9-based QF2 knock-in at the tyrosine hydroxylase (th) locus reveals novel th-expressing neuron populations in the zebrafish mid- and hindbrain

Zebrafish, Medaka and Turquoise Killifish for Understanding Human Neurodegenerative/Neurodevelopmental Disorders

Immunohistochemical characterisation of the adult Nothobranchius furzeri intestine

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