Orientation of Red Blood Cells and Rouleaux Disaggregation in Interference Laser Fields

Kruchenok, Julia V.; Бушук, С. Б.; Kurilo, G. I.; Nemkovich, N. A.; Рубинов, А. Н.

doi:10.1007/s10867-005-6732-6

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…12 The photobiomodulation effect induced by low power lasers is related to the monochromatic and collimated characteristics of laser beams, 13 which allow photon absorption by a specific photoacceptor 14 This effect has been reported to accelerate tissue repair and analgesia. 15,16 Also, photobiomodulation has been described in cells associated with inflammatory response and tissue repair, such as lymphocytes, 17 fibroblasts, 18 macrophages, 19 in the movement of red blood cells, 20 as well as in blood plasma components, such as hemoglobin, immunoglobulins and proteins, 21 cell growth factors 22 and cytokines. 23 Although therapeutic protocols have been used successfully, the mechanism of action of photobiomodulation has not been understood.…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of caspase 3 and Bcl-2 genes in acute lung injury

Sergio

Thomé

Trajano

et al. 2018

Photochem Photobiol Sci

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Acute respiratory distress syndrome (ARDS) and acute lung injury (ALI) are defined as pulmonary inflammation that could occur from sepsis and lead to pulmonary permeability and alveolar edema making them life-threatening diseases. Photobiomodulation (PBM) properties have been widely described in the literature in several inflammatory diseases; although the mechanisms of action are not always clear, this could be a possible treatment for ARDS/ALI. Thus, the aim of this study was to evaluate the mRNA levels from caspase-3 and BCL-2 genes and DNA fragmentation in lung tissue from Wistar rats affected by ALI and subjected to photobiomodulation by exposure to a low power infrared laser (808 nm; 100 mW; 3.571 W cm-2; four points per lung). Adult male Wistar rats were randomized into 6 groups (n = 5, for each group): control, PBM10 (10 J cm-2, 2 J and 2 seconds), PBM20 (20 J cm-2, 5 J and 5 seconds), ALI, ALI + PBM10 and ALI + PBM20. ALI was induced by intraperitoneal Escherichia coli lipopolysaccharide injection. Lung samples were collected and divided for mRNA expression of caspase-3 and Bcl-2 and DNA fragmentation quantifications. Data show that caspase-3 mRNA levels are reduced and Bcl-2 mRNA levels increased in ALI after low power infrared laser exposure when compared to the non-exposed ALI group. DNA fragmentation increased in inflammatory infiltrate cells and reduced in alveolar cells. Our research shows that photobiomodulation can alter relative mRNA levels in genes involved in the apoptotic process and DNA fragmentation in inflammatory and alveolar cells after lipopolysaccharide-induced acute lung injury. Also, inflammatory cell apoptosis is part of the photobiomodulation effects induced by exposure to a low power infrared laser.

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Section: Introductionmentioning

confidence: 99%

Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of caspase 3 and Bcl-2 genes in acute lung injury

Sergio

Thomé

Trajano

et al. 2018

Photochem Photobiol Sci

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“…LBI is a promising technology for the volume creation of a large regular array of optical traps with easily constructed and inexpensive lenses. This method does not require strongly focused beams and provides a way to generate periodical and quasi-periodical optical traps with adjustable periods and less damaging, so that simultaneous spatial manipulation of a large number of particles can be achieved [10]. However, compared with the current approaches, most of studies for the creation of multiple optical traps are limited to only create a pattern of linear interference fringes or a few of dot traps without the adjustment of the formation.…”

Section: Introductionmentioning

confidence: 99%

Capture and sorting of multiple cells by polarization-controlled three-beam interference

Hou

Wang

et al. 2016

J. Opt.

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For the capture and sorting of multiple cells, a sensitive and highly efficient polarizationcontrolled three-beam interference set-up has been developed. With the theory of superposition of three beams, simulations on the influence of polarization angle upon the intensity distribution and the laser gradient force change with different polarization angles have been carried out. By controlling the polarization angle of the beams, various intensity distributions and different sizes of dots are obtained. We have experimentally observed multiple optical tweezers and the sorting of cells with different polarization angles, which are in accordance with the theoretical analysis. The experimental results have shown that the polarization angle affects the shapes and feature sizes of the interference patterns and the trapping force.

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“…Drochon [8] obtained the relationship between the RBC velocity and deformed shape under different flow strengths, suspending medium viscosity and cell membrane elasticity using the cell transit analyzer. Kruchenok et al [9] observed the orientation of individual RBCs in interference fringes and found that RBC rouleaux undergo disaggregation under the action of interference laser fields. Lima et al [10] measured velocity distribution of RBC suspension flow in a microchannel with a 0.1 × 0.1 mm 2 cross-section by confocal micro-PIV.…”

Section: Introductionmentioning

confidence: 99%

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

2011

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The size of red blood cells (RBC) is on the same order as the diameter of microvascular vessels. Therefore, blood should be regarded as a two-phase flow system of RBCs suspended in plasma rather than a continuous medium of microcirculation. It is of great physiological and pathological significance to investigate the effects of deformation and aggregation of RBCs on microcirculation. In this study, a visualization experiment was conducted to study the microcirculatory behavior of RBCs in suspension. Motion and deformation of RBCs in a microfluidic chip with straight, divergent, and convergent microchannel sections have been captured by microscope and high-speed camera. Meanwhile, deformation and movement of RBCs were investigated under different viscosity, hematocrit, and flow rate in this system. For low velocity and viscosity, RBCs behaved in their normal biconcave disc shape and their motion was found as a flipping motion: they not only deformed their shapes along the flow direction, but also rolled and rotated themselves. RBCs were also found to aggregate, forming rouleaux at very low flow rate and viscosity. However, for high velocity and viscosity, RBCs deformed obviously under the shear stress. They elongated along the flow direction and performed a tank-treading motion.

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Orientation of Red Blood Cells and Rouleaux Disaggregation in Interference Laser Fields

Cited by 7 publications

References 23 publications

Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of caspase 3 and Bcl-2 genes in acute lung injury

Photobiomodulation prevents DNA fragmentation of alveolar epithelial cells and alters the mRNA levels of caspase 3 and Bcl-2 genes in acute lung injury

Capture and sorting of multiple cells by polarization-controlled three-beam interference

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

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