Origin and Specificity of Surface Immunoglobulin on MLR Activated Mouse T Cells

Nagy, Zoltán Á.; Elliott, B. E.; Nabholz, Markus; Krammer, Peter H.; Pernis, Benvenuto

doi:10.1016/b978-0-12-233750-5.50038-5

Cited by 12 publications

(24 citation statements)

References 8 publications

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“…The immunogenic stimulus for the T cell-macrophage interaction may be carried within the activated T cell population, perhaps in the form of an active antigen fragment carried by the T cells themselves or by the very few macrophages found as contaminants within the T cell population. Association of foreign alloantigens with the immune T cell surface has been reported (22 (1977) identify listerial antigens associated with immune T cells using anti-Listeria antibodies have failed. Alternatively, bovine proteins in the Listerla culture broth may be bound by the Listeria organisms, so that infection with Listeria also generates T cells reactive to bovine proteins.…”

Section: Discussionmentioning

confidence: 99%

Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex.

Farr¹,

Dorf²,

Unanue³

1977

Proc. Natl. Acad. Sci. U.S.A.

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In this study we show that T cells from mice infected with Listeria monocytogenes can interact in vitro with normal macrophages to pqoduce a number of soluble mediators, including loymphos imulayinolecules. One of these molecules was a 15,000 dalton protein mitogenic for thymocytes. Generation of mitogenic activity was essentially completed by the first 24 hr of culture and did not require the addition of Listeria antigens. Production of mitogenic protein required contact between the lymphocytes and macrophages, because it did not occur when the two cells were separated by a cell-impermeable membrane. Optimal production of mitogenic protein occurred only when-the lymphocytes and macrophages shared homologous I-A regions of the major histocompatibility complex. Once generated, the; mitogenic protein did not display histocompatibijity. restriction and could stimulate allogeneic as well as syngenelc thymocytes. Strains of mice with the C57 background responded oorly to mitogenic protein even though those strains were capable of producing it. We conclude that an early stage in T ce immunity to Listenia Iivolves an intimate association with macropliages regulated by the H-2 complex. It is becoming a*rent that most, if not all, of the responses of thymus-derive lymphocytes (T cells) require interaction with antigens that are, or have become associated with, cell surface components, more specifically, with products of the major histocompatibility complex (MHG) (1). Murine cytotoxic T cells readily recognize viral antigens (2-4), tumor-associated antigens (5-7), or chemically modified cell surface proteins (8), all of which appear to be associated with H-2K or H-2D products. There is also good evidence that recognition of soluble protein antigens by T cells, reflected by T cell proliferation, requires the involvement of nrtcrophages as antigen-presenting cells. In this case, the macrophages must bear critical I-region gene products for the T cell to respond (9, 10). Similar restrictions in T cell function have been shown in T-B cell collaboration (11) and in delayed sensitivity reactions (12). The implication from these observations is that-the surface molecules coded by the MHC gene complex represent key regulatory elements involved in the interaction between the T cell and the cell-bound antigen. The manner by which the MHC gene products regulate the interaction is not clear.The purpose of this paper is to report studies on T cellmacrophage interaction controlled by the MHC gene complex in which the immunizing antigen is the facultative intracellular bacterium Listeria monocytogenes. The specific immunity to Listeria organisms is conveyed by T cells that activate the mononuclear phagocyte system for increased bactericidal activity. It had been shown previously that addition of T cells from mice infected with Listeria monocytogenes to cultures of normal macrophages resulted in the increased secretion of a The costs of publication of this article were defrayed in part by the payment of page charges from funds m...

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Section: Discussionmentioning

confidence: 99%

Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex.

Farr¹,

Dorf²,

Unanue³

1977

Proc. Natl. Acad. Sci. U.S.A.

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“…For this reason, the positive effect of clone 86 derived lymphokines, readily detected in our in vitro model of altered self-cytolysis, is likely to be lost in the in vivo situation. These considerations, combined with the intriguing possibility of antigen binding and presentation by activated Th cells (18,19) (7).…”

Section: Discussionmentioning

confidence: 99%

“…These effector cells grow well in vitro and mediate extremely efficient cytolytic activity. While these allospecific Th cells function at least in part by the secretion of lymphokines, the capacity of Th cells to bind (18,19) and present (19) antigen to the immune system suggests that these cells may permit a novel immunotherapeutic strategy for human diseases. lated from non-T cells by E rosette formation with neuraminidasetreated sheep red blood cells and a second Ficoll-Hypaque centrifugation, as previously described (20).…”

Section: Introductionmentioning

confidence: 99%

Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells.

Friedman¹,

Green²,

Russo³

et al. 1988

J. Clin. Invest.

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“…The culture medium for MLR, anti-TNP response, and mitogen stimulation was RPMI 1640 (Gibco, Grand Island, NY) supplemented as described previously [20]. For the T cell proliferation assay (responses to G A and LDHB) the same medium supplemented with 5% horse serum (Gibco), instead of 10% fetal calf serum (FCS), was used.…”

Section: Cell-mediated Lympholysis (Cml)mentioning

confidence: 99%

“…T blast cells were obtained by centrifugation of cultures over Ficoll-Urovision [20]. To remove passively bound stimulator antigens [17,201 and self-Ia antigens [8,91, the cells were treated with trypsin, and incubated overnight in fresh medium containing IL2 (see Sect.…”

Section: Binding Assaysmentioning

confidence: 99%

T cell idiotypes recognizing self‐major histocompatibility complex molecules: H‐2 specificity, allotype linkage, and expression on functional T cell populations

et al. 1982

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An anti-idiotypic serum (antiserum 5936, B. Rubin et al., J. Exp. Med. 1979. 150: 307) was used to demonstrate receptor sites for self-major histocompatibility complex (MHC) antigens on T lymphocytes. The antiserum was raised by injecting rabbits tolerant to mouse Ig with a B6 anti-CBA (anti-H2k) alloantibody. It recognized a large proportion of T cells from H-2k strains carrying the b, c, d or e allele at the Igh-1 locus, but only a few T cells from H-2k strains with Igh-1 alleles a, f and j. Allotype linkage of the 5936 idiotype was also demonstrated by segregation analysis. The antiserum did not recognize either H-2k B cells or T cells from other H-2 haplotypes despite the presence of a permissive Igh-1 allele. The 5936 idiotype was found to be associated with several different antigen specificities, indicating that it is not located on the binding site for foreign antigen. Furthermore, the 5936 antiserum inhibited the binding of soluble Ik antigens by H-2k, Igh-1b, T cells, and, in the presence of complement, eliminated T cells responding to different antigens in an I-Ak-restricted fashion. Collectively, the data indicate that the structure bearing the 5936 idiotype is a receptor for I-Ak antigens, expressed by strains carrying the I-Ak allele and a permissive allele at the Igh-1 locus. The relevance of this finding to the MHC-restricted recognition of antigens by T cells is discussed.

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Origin and Specificity of Surface Immunoglobulin on MLR Activated Mouse T Cells

Cited by 12 publications

References 8 publications

Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex.

Secretion of mediators following T lymphocyte-macrophage interaction is regulated by the major histocompatibility complex.

Amplification of altered self-reactive cytolytic T lymphocyte responses by cloned, allospecific human Th cells.

T cell idiotypes recognizing self‐major histocompatibility complex molecules: H‐2 specificity, allotype linkage, and expression on functional T cell populations

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