Original article Comparative phytochemical screening, elemental content and chromatographic evaluation for detection and quantification of polyphenolic compounds for strong antioxidant activity of various extracts of Abutilon indicum (Link) Sweet leaves

Abstract

Abutilon indicum (Link) Sweet (AI) belongs to family Malvaceae. Traditionally, the plant was reported as healer for many ailments and other medicinal values such as leaves are effective against ulcer, laxative, expectorant, diuretic, astringent, analgesic, anti-inflammatory, anthelmintic, demulcent and aphrodisiac. Though, the plant is distributed in tropical and subtropical area, but is became threatened in Karnataka, India due to its habitat lost and over exploitation. The present experiment was carried out with a comparative study to investigate an effect of aqueous and methanol leaves extracts on presence of phytochemicals, vis-a-vis chromatographic evaluation for detection and quantification of gallic acid and quercetin for effective antioxidant activity. Metal ions such as Fe, Cu, Zn, Co, Ni, Cd, As, Hg were detected form dried leaves, using Atomic Absorption Spectrophotometer by acid digestion method. Microwave oven extraction was carried out, using both aqueous and methanol as solvents. The percentage yield was calculated and revealed maximum yield with methanol extract (18.62%). Various elements such as Fe, Zn, Cu were present in high amount in methanolic extract whereas Co, Ni, Cd, As, Pb, Hg were not detected in the leaves. Thereafter, phytochemical screening was carried out with various chemical tests and revealed presence of amino acids, carbohydrates, alkaloids, flavonoids, sterols, phenols and saponins with methanolic extract whereas amino acids, carbohydrates and flavonoids are present in aqueous extract. TLC study revealed presence of gallic acid and quercetin in methanol extract but gallic acid was absent in aqueous extract. Further, quantified by HPLC and showed higher amount of both the constituent in methanol extract (gallic acid 0.45 g/100 g and quercetin 0.179 g/100 g) than aqueous one (0.019 g/100 g of quercetin) where absence of gallic acid in aqueous extract. Furthermore, antioxidant study was carried out using DPPH, FRAP assay method and revealed strong free radical scavenging activity with methanol extract which showed positive significa nt correlation with element contents. Finally, results indicated that methanolic AI extract has strong antioxidant activity, may be due to presence of polyphenolic compounds such as gallic acid and quercetin.

Protective effect of leaf extract of Abutilon indicum on DNA damage and peripheral blood lymphocytes in combating the oxidative stress

“…Recent studies have revealed the cytoprotective efficacy of bioactive compounds from traditional medicinal plant extracts ( Song et al, 2019 , Dhanasekaran et al, 2012 ). The phenolic compounds found in Abutilon indicum are known as powerful chain-breaking antioxidants ( Das et al, 2019 ). Polyphenols are the major phytochemical constituents reported in Abutilon indicum that scavenge free radicals due to its hydroxyl group and may directly contribute to its antioxidative functions ( Tlili and Sarikurkcu, 2020 , Abcha et al, 2019 , Bendary et al, 2013 ).…”

Cancer preventive and neuroprotective potentials of red hulls, kernels and oleo-gum resins from Pistachio

“…By now, anticancer, antiproliferative, anticholinesterase, antityrosinase, antioxidant, and other biological activities of numerous medicinal and aromatic plants (MAPs) and secondary metabolites isolated from MAPs were analysed in our laboratory (Akgunlu et al, 2016;Sekeroglu et al, 2017;Gezici et al, 2017;Belkhodja et al, 2017;Karik et al, 2018;Gundogdu et al, 2018;Senol et al, 2018;Gezici, 2018;Das et al, 2019;Shida et al, 2019;Gezici and Sekeroglu, 2019a;Gezici and Sekeroglu, 2019b;Gezici, 2019a;Gezici 2019b;Gezici, 2019, Sekeroglu et al, 2019 in press). Take into consideration our ongoing projects, evaluation cancer protective potentials against human cancer cells, investigating neuroprotective activities through enzyme inhibitions on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and TYR (tyrosinase) enzymes, along with determination of antioxidant capacities of extracts obtained from red hulls, kernels and oleo-gum resins of Pistachio (P. vera L.) are the main objectives of the presented research.…”

Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays

“…Additionally, the phenolic compounds present in A. hirtum may play a critical role as antioxidants, which are considered strong chain breaker agents as suggested by Ref. [ 68 ]. This may explain the previous results in the current study and confirm the success of using the plant for medicinal purposes, with the need to adjust the therapeutic dose.…”