OriginPro 9.1: Scientific Data Analysis and Graphing Software—Software Review

Seifert, E.

doi:10.1021/ci500161d

Cited by 361 publications

(166 citation statements)

References 3 publications

Supporting

Mentioning

162

Contrasting

Unclassified

Order By: Relevance

“…Resulting activities were plotted against the ATP concentration and kinetic parameters were fitted using the Hill equation in the program ORIGIN (Seifert, 2014). …”

Section: Methodsmentioning

confidence: 99%

Structural basis for the disaggregase activity and regulation of Hsp104

Heuck

Schitter-Sollner

Suskiewicz

et al. 2016

eLife

View full text Add to dashboard Cite

The Hsp104 disaggregase is a two-ring ATPase machine that rescues various forms of non-native proteins including the highly resistant amyloid fibers. The structural-mechanistic underpinnings of how the recovery of toxic protein aggregates is promoted and how this potent unfolding activity is prevented from doing collateral damage to cellular proteins are not well understood. Here, we present structural and biochemical data revealing the organization of Hsp104 from Chaetomium thermophilum at 3.7 Å resolution. We show that the coiled-coil domains encircling the disaggregase constitute a ‘restraint mask’ that sterically controls the mobility and thus the unfolding activity of the ATPase modules. In addition, we identify a mechanical linkage that coordinates the activity of the two ATPase rings and accounts for the high unfolding potential of Hsp104. Based on these findings, we propose a general model for how Hsp104 and related chaperones operate and are kept under control until recruited to appropriate substrates.DOI: http://dx.doi.org/10.7554/eLife.21516.001

show abstract

“…Resulting activities were plotted against the ATP concentration and kinetic parameters were fitted using the Hill equation in the program ORIGIN (Seifert, 2014). …”

Section: Methodsmentioning

confidence: 99%

Structural basis for the disaggregase activity and regulation of Hsp104

Heuck

Schitter-Sollner

Suskiewicz

et al. 2016

eLife

View full text Add to dashboard Cite

show abstract

“…Subsequently, 300 points were marked in every contour line and the coordinates of each point were obtained and fitted to the curve by using Origin software and the existing function of egg shape (Seifert, 2014). The degree of coincidence between the two curves was determined using the simple Pearson correlation coefficient of these point sets.…”

Section: Function Of Goose Egg Shapementioning

confidence: 99%

Experimental Study on the Geometrical and Mechanical Properties of Goose Eggshells

Zhang

Peng

Tang

et al. 2017

Rev. Bras. Cienc. Avic.

View full text Add to dashboard Cite

This paper examined the properties of goose eggshells to determine possible areas of improvement in egg transport and storage. First, we measured goose egg sizes and performed statistical tests, and found that the major axis, minor axis, and egg-shape index presented normal distribution. Eggshell thickness first increased and then decreased from the blunt end to the sharp end. Second, the shape of individual goose eggshell was measured using a 3D scanner. Volume equation, surface equation, and contour function of goose eggshell shape were obtained, exhibiting a highly symmetrical structure. Finally, goose eggs were compressed along their major and minor axes between two plates. Breaking strength was highly dependent on the shape index. A crack was found on the force point along the major axis of each goose egg.

show abstract

“…The AMBER 14 integrated CPPTRAJ and PTRJ modules were used to analyze obtained coordinates and trajectories. The structural and visual analysis was done using the graphical user interface of UCSF Chimera, and data were plotted with MicroCal Origin data analysis software …”

Section: Methodsmentioning

confidence: 99%

Dynamic perspectives into the mechanisms of mutation‐induced p53‐DNA binding loss and inactivation using active perturbation theory: Structural and molecular insights toward the design of potent reactivators in cancer therapy

Olotu

2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

The DNA-binding ability of p53 represents the crux of its tumor suppressive activities, which involves transcriptional activation of target genes responsible for apoptosis and cell-cycle arrest. Mutational occurrences within or in close proximity to the DNA-binding surface of p53 have accounted for the loss of direct DNA-binding ability and inactivation implicated in many cases of cancer. Moreover, the design of therapeutic compounds that can restore DNA-binding ability in p53 mutants has been identified as a way forward in curtailing their oncogenic activities. However, there is still the need for more insights into evaluate the perturbations that occur at the DNA-binding interface of mp53 relative to DNA-binding loss, inactivation, and design of potent reactivators, hence the purpose of this study. Therefore, we evaluated p53-structural (R175H) and contact (R273C) mutational effects using tunnel perturbation analysis and other computational tools. We identified significant perturbations in the active tunnels of p53, which resulted in altered geometry and loss, unlike in the wild-type p53. This corroborated with structural, DNA-binding, and interaction network analysis, which showed that loss of flexibility, repulsion of DNA-interactive residues, and instability occurred at the binding interface of both mutants. Also, these mutations altered bonding interactions and network topology at the DNA-binding interface, resulting in the reduction of p53-DNA binding proximity and affinity. Therefore, these findings would aid the structure-based design of novel chemical entities capable of restoring p53-DNA binding and activation.

show abstract

OriginPro 9.1: Scientific Data Analysis and Graphing Software—Software Review

Cited by 361 publications

References 3 publications

Structural basis for the disaggregase activity and regulation of Hsp104

Structural basis for the disaggregase activity and regulation of Hsp104

Experimental Study on the Geometrical and Mechanical Properties of Goose Eggshells

Dynamic perspectives into the mechanisms of mutation‐induced p53‐DNA binding loss and inactivation using active perturbation theory: Structural and molecular insights toward the design of potent reactivators in cancer therapy

Contact Info

Product

Resources

About