Os<scp>HOP</scp>2 regulates the maturation of crossovers by promoting homologous pairing and synapsis in rice meiosis

Shi, Wenqing; Tang, Ding; Shen, Yi; Xue, Zhihui; Zhang, Fanfan; Zhang, Chao; Ren, Lijun; Liu, Changzhen; Du, Guijie; Li, Yafei; Yan, Changjie; Cheng, Zhukuan

doi:10.1111/nph.15664

Cited by 17 publications

(21 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, the expression of phosphatidylinositol 3 kinase-like (PI3K) protein kinase Ataxia-Telangiectasia Mutated ( ATM ), which undertakes a conserved function in sensing DNA damage and evoking DSB repair events (reviewed by (Paull, 2015)), is elevated under heat stress (Ning et al, 2021). In multiple species, the activity of ATM is negatively correlated with DSB abundance (Joyce et al, 2011; Lange et al, 2011; Zhang et al, 2011; Carballo et al, 2013; Garcia et al, 2015; Mohibullah and Keeney, 2017; Shi et al, 2019). In Arabidopsis, ATM limits DSB formation by restricting the accumulation of SPO11 on prophase I chromosomes (Yao et al, 2020; Kurzbauer et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Interfered Chromosome Pairing Promotes Meiosis Instability of Autotetraploid Arabidopsis by High Temperatures

Zhao

Ren

et al. 2021

Preprint

View full text Add to dashboard Cite

Alterations of environmental temperature affect multiple meiosis processes in flowering plants. Polyploid plants derived from whole genome duplication (WGD) have enhanced genetic plasticity and tolerance to environmental stress, but meanwhile face a challenge for organization and segregation of doubled chromosome sets. In this study, we investigated the impact of increased environmental temperature on male meiosis in autotetraploid Arabidopsis thaliana. Under low to mildly-increased temperatures (5-28°C), irregular chromosome segregation universally takes place in synthesized autotetraploid Columbia-0 (Col-0). Similar meiosis lesions occur in autotetraploid rice (Oryza sativa L.) and allotetraploid canola (Brassica napus cv. Westar), but not in evolutionary-derived hexaploid wheat (Triticum aestivum). As temperature increases to extremely high, chromosome separation and tetrad formation are severely disordered due to univalent formation caused by suppressed crossing-over. We found a strong correlation between tetravalent formation and successful chromosome pairing, both of which are negatively correlated with temperature elevation, suggesting that increased temperature interferes with crossing-over prominently by impacting homolog pairing. Besides, we showed that loading irregularities of axis proteins ASY1 and ASY4 co-localize on the chromosomes of syn1 mutant, and the heat-stressed diploid and autotetraploid Col-0, revealing that heat stress affects lateral region of synaptonemal complex (SC) by impacting stability of axis. Moreover, we showed that chromosome axis and SC in autotetraploid Col-0 are more sensitive to increased temperature than that of diploid Arabidopsis. Taken together, our study provide evidence suggesting that WGD without evolutionary and/or natural adaption negatively affects stability and thermal tolerance of meiotic recombination in Arabidopsis thaliana.

show abstract

Section: Discussionmentioning

confidence: 99%

Interfered Chromosome Pairing Promotes Meiosis Instability of Autotetraploid Arabidopsis by High Temperatures

Zhao

Ren

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Recently, the interaction of OsHOP2 and OsZIP1 was reported, indicating that OsHOP2 play a key role in facilitating pairing of synapsis and the formation of CO (Shi et al, 2019). Obviously, not all OsHOP2 form a complex with OsMFS1.…”

Section: Discussionmentioning

confidence: 99%

OsMFS1/OsHOP2 Complex Participates in Rice Male and Female Development

Wang

et al. 2020

Front. Plant Sci.

View full text Add to dashboard Cite

Meiosis plays an essential role in the production of gametes and genetic diversity of posterities. The normal double-strand break (DSB) repair is vital to homologous recombination (HR) and occurrence of DNA fragment exchange, but the underlying molecular mechanism remain elusive. Here, we characterized a completely sterile Osmfs1 (male and female sterility 1) mutant which has its pollen and embryo sacs both aborted at the reproductive stage due to severe chromosome defection. Mapbased cloning revealed that the OsMFS1 encodes a meiotic coiled-coil protein, and it is responsible for DSB repairing that acts as an important cofactor to stimulate the single strand invasion. Expression pattern analyses showed the OsMFS1 was preferentially expressed in meiosis stage. Subcellular localization analysis of OsMFS1 revealed its association with the nucleus exclusively. In addition, a yeast two-hybrid (Y2H) and pulldown assay showed that OsMFS1 could physically interact with OsHOP2 protein to form a stable complex to ensure faithful homologous recombination. Taken together, our results indicated that OsMFS1 is indispensable to the normal development of anther and embryo sacs in rice.

show abstract

“…RAD51 and DMC1 also associate with other proteins modulating their activities such as BRCA2 ( Siaud et al, 2004 ; Dray et al, 2006 ; Seeliger et al, 2012 ; Fu et al, 2020 ), FIGL1 and FLIP ( Zhang P. et al, 2017 ; Fernandes et al, 2018a ; Kumar et al, 2019 ), or MND1, and HOP2 ( Vignard et al, 2007 ; Uanschou et al, 2013 ; Aklilu et al, 2020 ). Rice MND1/MSF1 can interact with RPA2b and HOP2 ( Lu et al, 2020 ), while OsHOP2 can directly interact with the SC central element ZEP1/ZYP1 suggesting a second mechanism for the link observed between early recombination and synapsis ( Shi et al, 2019 ) at the SEI step. The excess number of SEI creating several interconnections between homologous chromosomes (also called interhomolog joint molecules) were proposed to be involved in the pairing process ( Pawlowski et al, 2003 ).…”

Section: Single-end Invasions and Pairingmentioning

confidence: 99%

The Formation of Bivalents and the Control of Plant Meiotic Recombination

et al. 2021

View full text Add to dashboard Cite

During the first meiotic division, the segregation of homologous chromosomes depends on the physical association of the recombined homologous DNA molecules. The physical tension due to the sites of crossing-overs (COs) is essential for the meiotic spindle to segregate the connected homologous chromosomes to the opposite poles of the cell. This equilibrated partition of homologous chromosomes allows the first meiotic reductional division. Thus, the segregation of homologous chromosomes is dependent on their recombination. In this review, we will detail the recent advances in the knowledge of the mechanisms of recombination and bivalent formation in plants. In plants, the absence of meiotic checkpoints allows observation of subsequent meiotic events in absence of meiotic recombination or defective meiotic chromosomal axis formation such as univalent formation instead of bivalents. Recent discoveries, mainly made in Arabidopsis, rice, and maize, have highlighted the link between the machinery of double-strand break (DSB) formation and elements of the chromosomal axis. We will also discuss the implications of what we know about the mechanisms regulating the number and spacing of COs (obligate CO, CO homeostasis, and interference) in model and crop plants.

show abstract

OsHOP2 regulates the maturation of crossovers by promoting homologous pairing and synapsis in rice meiosis

Cited by 17 publications

References 64 publications

Interfered Chromosome Pairing Promotes Meiosis Instability of Autotetraploid Arabidopsis by High Temperatures

Interfered Chromosome Pairing Promotes Meiosis Instability of Autotetraploid Arabidopsis by High Temperatures

OsMFS1/OsHOP2 Complex Participates in Rice Male and Female Development

The Formation of Bivalents and the Control of Plant Meiotic Recombination

Contact Info

Product

Resources

About