OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis

Li, Jinjie; Guo, Xuewu; Zhang, Minghui; Wang, Xin; Zhao, Yan; Zhen, Yin; Zhang, Zhanying; Wang, Yanming; Xiong, Haiyan; Zhang, Hongliang; Todorovska, Elena; Li, Zichao

doi:10.1016/j.plantsci.2018.01.017

Cited by 92 publications

(58 citation statements)

References 53 publications

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“…However, the MDA content was significantly lower in OsEXPA7-OX plants than in WT plants, which suggests reduced lipid peroxidation in transgenic plants under 150 mM salt stress ( Figure 6D). Proline, which is an amino acid, acts as a beneficial solute to increase cellular osmolarity during abiotic stress, by balancing the cytoplasmic osmotic potential and scavenging ROS [44]. The increase in proline accumulation was much higher in OsEXPA7-OX plants than in WT plants after salt treatment ( Figure 6E).…”

Section: Overexpression Of Osexpa7 Improved the Antioxidant Capacity mentioning

confidence: 96%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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Expansins are key regulators of cell-wall extension and are also involved in the abiotic stress response. In this study, we evaluated the function of OsEXPA7 involved in salt stress tolerance. Phenotypic analysis showed that OsEXPA7 overexpression remarkably enhanced tolerance to salt stress. OsEXPA7 was highly expressed in the shoot apical meristem, root, and the leaf sheath. Promoter activity of OsEXPA7:GUS was mainly observed in vascular tissues of roots and leaves. Morphological analysis revealed structural alterations in the root and leaf vasculature of OsEXPA7 overexpressing (OX) lines. OsEXPA7 overexpression resulted in decreased sodium ion (Na+) and accumulated potassium ion (K+) in the leaves and roots. Under salt stress, higher antioxidant activity was also observed in the OsEXPA7-OX lines, as indicated by lower reactive oxygen species (ROS) accumulation and increased antioxidant activity, when compared with the wild-type (WT) plants. In addition, transcriptional analysis using RNA-seq and RT-PCR revealed that genes involved in cation exchange, auxin signaling, cell-wall modification, and transcription were differentially expressed between the OX and WT lines. Notably, salt overly sensitive 1, which is a sodium transporter, was highly upregulated in the OX lines. These results suggest that OsEXPA7 plays an important role in increasing salt stress tolerance by coordinating sodium transport, ROS scavenging, and cell-wall loosening.

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Section: Overexpression Of Osexpa7 Improved the Antioxidant Capacity mentioning

confidence: 96%

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Jadamba

Kang

Paek

et al. 2020

IJMS

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“…In addition, numerous studies have proposed that plenty of AP2/ERF TFs respond to phytohormones ethylene and abscisic acid (ABA), thereby activating ethylene‐ and ABA‐dependent stress response pathways (Xie et al., 2019). For instance, OsERF71 confers high salinity and drought tolerance by regulating ABA signaling and proline synthesis (Li et al., 2018). Overall, the capability to respond to various stresses and integrate multiple stress signals makes AP2/ERF TFs form a complicated regulatory network, thus AP2/ERF TFs are prospective candidate factors for crop genetic engineering to increase yield under abiotic stress.…”

Section: Introductionmentioning

confidence: 99%

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

et al. 2020

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The AP2/ERF transcription factors (TFs) regulate various processes of plant growth, development, and response to environmental stimuli. Although they have been extensively documented in many plants, little is known about stress responsive APETALA2/ethylene response factor (AP2/ERF) genes in sweetpotato (Ipomoea batatas L.). In this study, 20 putative AP2/ERF genes, named IbERF1, IbERF2, and IbERF5–IbERF23 were identified based on the salt‐treated RNA‐seq data from two sweetpotato cultivars with different salt tolerance. Phylogenetic analysis and sequence characterization identified that all the screened IbERF proteins contained a conserved AP2 domain, which belonged to dehydration‐responsive element binding proteins (DREB; 7 members) or ERF (13 members) subfamily. The systematic expression analysis of 12 mainly salt‐induced IbERFs by quantitative real‐time polymerase chain reaction (qRT‐PCR) revealed that most IbERFs were remarkably increased by multiple abiotic stresses, such as NaCl, dehydration, and cold stress. Furthermore, varying degrees of upregulation were also detected in the expression of most IbERFs when treated with hormone abscisic acid, jasmonic acid, and salicylic acid. The expression profiles of IbERFs corroborate their involvement in diverse biological processes. In addition, the IbERF5, which was exclusively upregulated by the treatments, was identified as a nuclear protein without transcriptional activation activity. Collectively, the data of stress responsive IbERFs will provide valuable knowledge for further functional studies of IbERFs in sweetpotato stress tolerance.

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“…In addition, the ERF genes also play a key role in plant growth and development. For example, MdERF1B can regulate the biosynthesis of apple anthocyanin and procyanidins (Zhang et al, 2018), and OsERF1 significantly affects the growth and development of transgenic Arabidopsis (Hu et al, 2008). However, no ERF family genes have been reported to be involved in the response to Al stress in plants.…”

Section: Discussionmentioning

confidence: 99%

GsERFenhances aluminum tolerance through an ethylene-mediated pathway inArabidopsis thaliana

Nian

et al. 2020

Preprint

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AbstractThe ethylene response factor (ERF) transcription factor is a subfamily of AP2/ERF superfamily in plants, which plays multiple roles in plant growth and development as well as stress response. In this study, we found that the GsERF gene from BW69 line of wild soybean held a constitutive expression pattern and induced by aluminum stress with more transcripts in soybean root. The putative GsERF protein containing an AP2 domain was in the nucleus and transactivation activity. In addition, the overexpression of the GsERF gene enhanced root relative length rate in Arabidopsis and shallow staining by hematoxylin under the treatments of AlCl3. The ethylene synthesis related genes such as ACS4, ACS5 and ACS6 are upregulated in the GsERF overexpressed plants than those in wild type plants under the treatment of AlCl3. Furthermore, expression levels of stress/ABA-responsive marker genes, including ABI1, ABI2, ABI4, ABI5, RD29B and RD22 in transgenic lines compared with those in wild type Arabidopsis were affected by AlCl3 treatments. Taken together, the results indicate that overexpression of GsERF may enhance aluminum tolerance through an ethylene-mediated pathway and/or ABA signaling pathway in Arabidopsis thaliana.

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OsERF71 confers drought tolerance via modulating ABA signaling and proline biosynthesis

Cited by 92 publications

References 53 publications

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Overexpression of Rice Expansin7 (Osexpa7) Confers Enhanced Tolerance to Salt Stress in Rice

Identification, expression analysis, and functional characterization of salt stress‐responsive genes of AP2/ERF transcription factors in sweetpotato

GsERFenhances aluminum tolerance through an ethylene-mediated pathway inArabidopsis thaliana

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