OsMATL mutation induces haploid seed formation in indica rice

Yao, Li; Zhang, Ya Ping; Liu, Chunxia; Liu, Yubo; Wang, Yanli; Liang, Dan; Liu, Juntao; Sahoo, Gayatri; Kelliher, Timothy

doi:10.1038/s41477-018-0193-y

Cited by 211 publications

(134 citation statements)

References 19 publications

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“…The authors edited PAIR1 , OsREC8 , OsOSD1 , and OsMATL in the F1 generation (Wang et al ). However, consistent with previously reported, mutation of OsMATL gives rise to a low fertility and low haploid or apomictic induction rate (Yao et al ; Wang et al ). These defects could be great obstacles to the application of such strategies.…”

Section: Resultssupporting

confidence: 92%

A strategy for generating rice apomixis by gene editing

Xie

Tang

et al. 2019

JIPB

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Summary Apomixis is an asexual reproduction way of plants that can produce clonal offspring through seeds. In this study, we introduced apomixis into rice (Oryza sativa) by mutating OsSPO11‐1, OsREC8, OsOSD1, and OsMATL through a CRISPR/Cas9 system. The quadruple mutant showed a transformation from meiosis to mitosis and produced clonal diploid gametes. With mutated Osmatl, which gives rise to haploid induction in plants, the quadruple mutant is expected to be able to be produced apomictic diploid offspring. We named this quadruple mutant as AOP (Apomictic Offspring Producer) for its ability to produce apomictic offspring.

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Section: Resultssupporting

confidence: 92%

A strategy for generating rice apomixis by gene editing

Xie

Tang

et al. 2019

JIPB

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“…A recent study showed that haploidy inducer lines of rice can be established via knockout of the rice ortholog of the maize MATL gene. When the matl mutant lines were used as pollinators, as much as 6% of the progeny proved to be haploids (Yao et al ).…”

Section: Applications Of Targeted Genome Modification By Nhejmentioning

confidence: 99%

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Kumlehn

Pietralla

Hensel

et al. 2018

JIPB

102

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Since the discovery that nucleases of the bacterial CRISPR (clustered regularly interspaced palindromic repeat)‐associated (Cas) system can be used as easily programmable tools for genome engineering, their application massively transformed different areas of plant biology. In this review, we assess the current state of their use for crop breeding to incorporate attractive new agronomical traits into specific cultivars of various crop plants. This can be achieved by the use of Cas9/12 nucleases for double‐strand break induction, resulting in mutations by non‐homologous recombination. Strategies for performing such experiments − from the design of guide RNA to the use of different transformation technologies − are evaluated. Furthermore, we sum up recent developments regarding the use of nuclease‐deficient Cas9/12 proteins, as DNA‐binding moieties for targeting different kinds of enzyme activities to specific sites within the genome. Progress in base deamination, transcriptional induction and transcriptional repression, as well as in imaging in plants, is also discussed. As different Cas9/12 enzymes are at hand, the simultaneous application of various enzyme activities, to multiple genomic sites, is now in reach to redirect plant metabolism in a multifunctional manner and pave the way for a new level of plant synthetic biology.

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“…The observed phenotype of TaPLAs knockout lines including a ~2-3% haploid frequency and significantly decreased SSR, are similar to that of maize and rice (Gilles et al, 2017; Kelliher et al, 2017; Liu et al, 2017a; Yao et al, 2018), suggesting that MTL /Zm PLA1 / NLD homologues are involved in the similar pathway during double-fertilization across different crop species. Thus, our study provided an alternative crop species in exploring the biological mechanism of haploid induction.…”

Section: Resultsmentioning

confidence: 61%

“…Loss of function of the gene MTL / ZmPLA1 / NLD in qhir1 triggers haploid induction (Gilles et al, 2017; Kelliher et al, 2017; Liu et al, 2017a). Importantly, in vivo haploid induction system had been successfully applied to rice (Yao et al, 2018), making this method more important in plant breeding. Genetic analysis on the QTLs contributing HIR revealed that qhir8 can improve haploid induction efficiency by 3- to 5- fold (Liu et al, 2015), this is a promising result for improving DH breeding efficiency in crop species other than maize.…”

Section: Introductionmentioning

confidence: 99%

Extension of thein vivohaploid induction system from maize to wheat

Liu

Zhong

et al. 2019

Preprint

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3Doubled haploid breeding technology has been one of the most important techniques for 1 4 accelerating crop breeding. In compare to in vivo haploid induction in maize, which is efficient 1 5 and background independent, wheat haploid production by interspecific hybridization pollinated 1 6 with maize is influenced by genetic background and requires rescue of young embryos. Here, we 1 7 analyzed the homologues of maize haploid induction gene MTL/ZmPLA1/NLD in several crop 1 8 species systematically, the homologues are highly conserved in sorghum, millet and wheat etc. 1 9Since wheat is a very important polyploidy crop, as a proof of concept, we demonstrated that the 2 0 in vivo haploid induction method could be extended from diploid maize to hexaploid wheat by 2 1 knocking out the wheat homologues (TaPLAs). Result showed that double knock-out mutation 2 2 could trigger wheat haploid induction at ~ 2%-3%, accompanied by 30% -60% seed setting rate. 3The performance of haploid wheat individual showed shorter plant, narrower leaves and male 2 4 sterile. Our results also revealed that knockout of TaPLA-A and TaPLA-D do not affect pollen 2 5 viability. This study not only confirmed the function of the induction gene and explored a new 2 6approach for haploid production in wheat, but also provided an example that the in vivo haploid 2 7 induction could be applied in more crop species with different ploidy levels. Furthermore, by 2 8 combining with gene editing, it would be a fast and powerful platform for traits improvement in 2 9 polyploidy crops breeding. 3 0

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OsMATL mutation induces haploid seed formation in indica rice

Cited by 211 publications

References 19 publications

A strategy for generating rice apomixis by gene editing

A strategy for generating rice apomixis by gene editing

The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology

Extension of thein vivohaploid induction system from maize to wheat

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