Osmo- und Volumenregulation beiHirudo medicinalis nach Nahrungsaufnahme

Zerbst‐Boroffka, Irene

doi:10.1007/bf00697606

Cited by 37 publications

(16 citation statements)

References 11 publications

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“…20%, is attributed to the conversion of AI into AII, of AII into AIII and of AIII into AIV, the three active peptides in leech osmoregulation [1]. These results are in line with those of Zerbst-Boroffka [20]. This author has demonstrated by physiological experiments conducted in Hirudo medicinalis an 8-fold increase of the urine volume excreted 15 min after a blood meal, which persisted for many hours.…”

Section: Resultsmentioning

confidence: 56%

“…Western blot analyses *Corresponding author. Fax: (33) (20) 43-4054. E-mail: salzet@pop.univ-lille 1. fr Abbreviations: AI, angiotensin I; AII, angiotensin II; AIl-amide, angiotensin II-amide; a-AI, anti-angiotensin l; ACE, angiotensinconverting enzyme; AP, aminopeptidase; DIA, dot immunobinding assay; HPLC, high performance liquid chromatography; NEP, endopeptidase-24.11; RAS, renin-angiotensin system; TBS, Tris/HC1 buffer saline; NAP, neutral aminopeptidase; DPAP, dipeptidyl aminopeptidase; Arg-AP, arginyl-aminopeptidase; Asp-AP, asparagyl-aminopeptidase.…”

Section: Introductionmentioning

confidence: 99%

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Metabolism of angiotensins by head membranes of the leech Theromyzon tessulatum

Laurent

1996

FEBS Letters

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Angiotensins (angiotensin I, angiotensin II, angiotensin II-amide) have been isolated in leeches and such peptides are involved in diuresis in these animals. To explore possible inactivation mechanisms of these peptides, angiotensins were incubated with head membranes of the leech T. tessulatum. Membranes derived from head parts of this leech are very rich in peptidases. They contain endopeptidase-24.11-1ike enzyme (NEP-like) associated with a battery of exopeptidase. The way that angiotensins are degraded by the combined attack of these membrane peptidases has been investigated. The contribution of individual peptidases was assessed by adding inhibitors (phosphoramidon, captopril and amastatin) to the membrane fractions, when they were incubated with the peptides. In the case of angiotensin I, the primary attack was performed by a combined action of the NEP-like and the ACE-like enzymes, followed by aminopeptidase attacks. Angiotensin II and III were hydrolyzed by NEP-like enzyme at the same Tyr-Ile bond, whereas the Nterminal arginine residue of angiotensin IH was removed by an arginyl aminopeptidase. These results show that angiotensins are efficiently degraded by membranes and that NEP-like enzyme plays a key role in this process.

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Section: Resultsmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Metabolism of angiotensins by head membranes of the leech Theromyzon tessulatum

Laurent

1996

FEBS Letters

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“…A number of morphological and physiological features may allow leeches to retain the ability to swim after feeding. Leeches have highly extensible body wall muscles (Wilson et al , 1996) with broad length tension curves (Miller, 1975), and are capable of high rates of urine production (Zerbst‐Boroffka, 1973). Such features could allow for the maintenance of muscle contractility during distension of the body wall and the rapid reduction of body volume post‐feeding, favoring the maintenance and/or restoration of swimming ability after a meal.…”

Section: Introductionmentioning

confidence: 99%

Effects of feeding on medicinal leech swimming performance

et al. 2009

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The locomotor system of sanguivorous leeches is presented with a unique challenge: how to maintain mobility while coping with a 4500% increase in body mass during feeding. A meal of this size is likely to disrupt the function of the muscular hydrostat during swimming, reducing speed and increasing predation risks. We quantified the effects of feeding to satiety on swimming kinematics, and the time course of recovery of swimming performance post-feeding in the medicinal leech Hirudo verbana. There was a 5.07 AE 0.04-fold increase in mass during feeding (mean AE SEM, n= 7). Despite this, leeches were able to swim immediately after feeding, reaching 27% of their pre-feeding speed. Reduced speed was a consequence of a reduction in both swimming cycle frequency and stride length to 69 and 42% of the pre-feeding values, respectively. Recovery of swimming ability was rapid, despite a prolonged increase in body mass. Fifty per cent restoration of swimming speed was achieved in c. 1 h while body mass was still 4.2-fold greater than before feeding. Rapid mass and volume reduction immediately post-feeding, and the properties of the obliquely striated swimming muscles appear to aid recovery of swimming performance. Such features that aid postfeeding recovery of mobility may have been important in the evolution of leech sanguivory.

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“…Leeches were prescreened the day before filming to ensure that the skin pigmentation was light enough to visualize the hearts. Any host blood in the crop, which would also interfere with imaging, was removed by lavage through the mouth (for a detailed description see Wenning et al 1980;Zerbst-Boroffka 1973). The animal was freed and placed in artificial pond water overnight and imaged the next or a later day.…”

Section: Animals and Solutionsmentioning

confidence: 99%