Osmotic effect of choline and glycine betaine on the gills and hepatopancreas of the Chasmagnathus granulata crab submitted to hyperosmotic stress

Jahn, Matheus Parmegiani; Cavagni, Gabriela Maura; Kaiser, D L; Kucharski, Luiz Carlos

doi:10.1016/j.jembe.2006.01.006

Cited by 28 publications

(25 citation statements)

References 30 publications

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“…According to Rand et al (2000), the study on osmoregulation gave rise to a multitude of analyses at various levels of biological organization (i.e. organisms, cells and molecules), especially with coastal marine invertebrates, which, being often exposed to salinity variations in their natural habitat, are good models for the study about the effects of osmolarity changes.…”

Section: Discussionmentioning

confidence: 99%

“…The study showed that in invertebrates, just as vertebrates and micro-organisms, to overcome a hypoosmotic shock that causes water input that may lead to osmolysis, the cells will release organic osmolytes, especially free amino acids and their derivatives, through its membrane (JAHN et al, 2006), which explains the morphological modifications in zoanthid P. variabilis. Reimer (1971) showed that in zoanthids, the opening of oral disc is related to peptides.…”

Section: Discussionmentioning

confidence: 99%

“…Environmental factors, such as drought, salinity and temperature extremes, are limiting factors for species survival. Organisms that live in habitats where these factors are a major issue have developed some adaptations to survive in these environments (JAHN et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Acclimation responses increase the overall capacity of an organism to perform a physiological function. The acclimation response is similar or identical to phenotypic plasticity; its presence or absence often determines the capacity of an animal to live in certain habitats and, thus, determines the ecological limits to species distributions (MCCORMICK; BRADSHAW, 2006 respect to osmotic regulation: the "strong regulators", which can maintain their hemolymph osmolality almost constant in a wide range of external salinity values, and the "weak regulators", which only osmoregulate within a narrow range of external salinity values and whose hemolymph osmolality drops, more or less in parallel with the isosmotic line, when the medium salinity falls below a certain limit (PRUSCH, 1983). The Order Zoanthidea members are colonial anthozoarians, usually connected through a stolon on their lower parts.…”

Section: Introductionmentioning

confidence: 99%

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Osmoregulation in tropical zoanthid Protopalythoa variabilis (Cnidaria: Anthozoa)

Soares

Souza

2013

Acta Sci. Biol. Sci.

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ABSTRACT. This study evaluated the physiological, morphological and ethological effects of salinity variations on the tropical zoanthid Protopalythoa variabilis. This zoanthid was submitted to different salinity levels to probe the hypothesis of osmoregulation. Specimens collected in beach rocks from Northeastern Brazil were taken alive to the laboratory in their original water. The osmoregulatory ability of P. variabilis can be determined by measuring the hemolymph osmolality under various salinity conditions and comparing with the osmolality of the medium. The zoanthid P. variabilis is a "weak regulator", as it only osmoregulates within a narrow range of external salinity values, and its hemolymph osmolality drops, approximately in parallel with the isosmotic line, when the medium salinity falls below a certain limit. Ethological and morphological modifications under different salinities are discussed. This experiment shows for the first time the importance of osmotic regulation in the tropical zoanthid P. variabilis.Keywords: anthozoan, salinity, physiology, reefs. Osmorregulação no zoantídeo tropical Protopalythoa variabilis (Cnidaria: Anthozoa) RESUMO. Este estudo avaliou os efeitos fisiológicos, morfológicos e etológicos no zoantídeo tropicalProtopalythoa variabilis em diferentes níveis de salinidade. Para testar a hipótese de osmorregulação, o zoantídeo foi submetido a diferentes variações de salinidade. Os indivíduos foram coletados em recifes de arenito no Nordeste brasileiro e foram levados vivos para o laboratório na água em que foram recolhidos. A capacidade osmorregulatória de P. variabilis foi determinada pela medição da osmolaridade da hemolinfa em diferentes condições de salinidade e comparadas com a osmolaridade do meio. O zoantídeo P. variabilis é um "regulador fraco" visto que osmorregula dentro de uma estreita faixa de valores de salinidade externa e quando a salinidade do meio cai abaixo de um certo limite, ocorre redução da osmolaridade da hemolinfa, aproximadamente em paralelo com a linha isosmótica. As mudanças etológicas e morfológicas em diferentes salinidades são discutidas. Este experimento demonstra pela primeira vez a importância da regulação osmótica no zoantídeo tropical P. variabilis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Osmoregulation in tropical zoanthid Protopalythoa variabilis (Cnidaria: Anthozoa)

Soares

Souza

2013

Acta Sci. Biol. Sci.

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“…Hyper-osmoregulation requires the involvement of various branchial ion transport mechanisms such as Na + /K + -ATPase activity and carbonic anhydrase, although the extent, timing and activity in individual gills can be speciesspecific; hypo-osmoregulation responses appear more variable and may require different mechanisms (López Mañanes et al 2000, 2002, Schleich et al 2001, Bianchini et al 2008, Freire et al 2008, McNamara and Faria 2012, Romano and Zeng 2012. Biochemical adaptation to environmental salinity appears to be a complex process involving also the participation of enzymes and transport systems in extrabranchial tissues (Jahn et al 2006, Pinoni and López Mañanes, 2004, 2009, Martins et al 2011. The biochemical changes occurring in salinity adaptation require a metabolic reorganization to support increasing energy demands (Freire et al 2008, McNamara and Faria 2012, Romano and Zeng 2012.…”

Section: Introductionmentioning

confidence: 99%

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

Michiels¹,

Valle²,

Mañanes³

2012

Sci. Mar.

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SUMMARY:We studied the occurrence and characteristics of lipase activity and the response of lipase and proteolytic activity to salinity and dopamine injections in hepatopancreas of the euryhaline crab (Cyrtograpsus angulatus). Lipase activity was maximal at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent K m =0.019 mM), was higher at 37°C and appeared to be cold tolerant, being also high at 4°C. In 10 psu (hyper-regulation conditions), lipase and proteolytic activity were about 3 and 5 times higher, respectively, than in 35 psu (osmoconformation). In 40 psu (hypo-regulation), lipase activity was about three times higher than in 35 psu, while proteolytic activity was similar. Lipase activity was inhibited in vivo by 10 -4 M dopamine in 35 psu but not in 10 or 40 psu. Proteolytic activity was not affected by 10 -4 M dopamine. The differential responses of lipase and proteolytic activity to salinity and dopamine suggest the occurrence of distinct digestive adjustments and mechanisms of regulation upon osmoregulatory conditions. This study contributes to a better understanding of the complexity of the biochemical adaptations to salinity in euryhaline crabs. The fact that higher digestive enzyme activities could be associated with a differential digestive capacity potentially leading to enhanced availability of energy substrates is discussed.Keywords: Cyrtograpsus angulatus, euryhaline crabs, salinity, hepatopancreas, digestive enzymes, dopamine, Mar Chiquita lagoon. RESUMEN: EfEcto dE la salinidad ambiEntal y dE la inyEcción dE dopamina sobrE Enzimas digEstivas clavE En hEpatopáncrEas dEl cangrEjo EurihalinoCyrtograpsus angulatus (dEcapoda: brachyura: varunidaE). -Se estudió la existencia y características bioquímicas de actividad de lipasa y la respuesta a salinidad y a dopamina de actividad de lipasa y proteolítica total en el hepatopáncreas del cangrejo eurihalino Cyrtograpsus angulatus. La actividad de lipasa fue máxima a pH 8,5; mostró una cinética michaeliana (K m =0.019 mM); fue mayor a 37°C, y resistente a baja temperatura (4°C). En 10 psu (condición de hiperregulación), las actividades de lipasa y proteolítica fueron 3 y 5 veces mayores, respectivamente, que en 35 psu (condición de osmoconformación). En 40 psu (condición de hiporregulación), la actividad de lipasa fue de alrededor 3 veces mayor que en individuos aclimatados a 35 psu, mientras que la actividad proteolítica fue similar. La actividad de lipasa fue inhibida in vivo por dopamina 10 -4 M en 35 psu pero no fue afectada en 10 o 40 psu. Dopamina 10 -4 M no afectó la actividad proteolítica. Las respuestas de las actividades de lipasa y proteolítica a salinidad y a dopamina permiten sugerir la existencia de ajustes digestivos y mecanismos de regulación diferenciales en relación al estado osmorregulatorio. Los resultados constituyen un aporte relevante al conocimiento sobre las complejas adaptaciones a nivel bioquímico a la salinidad en cangrejos eurihalinos.

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Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

2013

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This study constitutes a first attempt to investigate intraspecific differences in osmoregulatory capacity and digestive and metabolic responses at the biochemical level in relation to hyper-and hypo-regulation in a single species of estuarine crab inhabiting contrasting habitats within a same intertidal area. We compared hemolymph osmolality, key digestive enzymes, glycemia and energy reserves in Neohelice granulata (Dana in Proc Acad Nat Sci Philadelphia 5:247-254, 1851) from the mudflat and saltmarsh of Mar Chiquita coastal lagoon (37°32 0 /37°45 0 S-57°19 0 /57°26 0 W) under a wide range of salinities (6-60 psu). Individuals from both sites exhibited high and similar osmoregulatory capacity, but while in individuals from mudflat low and high salinities affected lipase activity in hepatopancreas and triglycerides in muscle, in crabs from saltmarsh, high salinities affected glycogen in anterior gills. Low salinity differentially affected free glucose in anterior gills. The results suggest the occurrence of intraspecific distinct digestive and metabolic adjustments in relation to osmoregulatory responses and habitat.

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Osmotic effect of choline and glycine betaine on the gills and hepatopancreas of the Chasmagnathus granulata crab submitted to hyperosmotic stress

Cited by 28 publications

References 30 publications

Osmoregulation in tropical zoanthid Protopalythoa variabilis (Cnidaria: Anthozoa)

Osmoregulation in tropical zoanthid Protopalythoa variabilis (Cnidaria: Anthozoa)

Effect of environmental salinity and dopamine injections on key digestive enzymes in hepatopancreas of the euryhaline crab <i>Cyrtograpsus angulatus</i> (Decapoda: Brachyura: Varunidae)

Phenotypic flexibility in response to environmental salinity in the euryhaline crab Neohelice granulata from the mudflat and the saltmarsh of a SW coastal lagoon

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