Osteogenic effects of bioactive glass on bone marrow stromal cells

Radin, S.; Reilly, Gwendolen C.; Bhargave, Geeta; Leboy, Phoebe S.; Ducheyne, Paul

doi:10.1002/jbm.a.30241

Cited by 87 publications

(88 citation statements)

References 28 publications

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“…The enhanced bone growth induced by BG results in a multistage mechanism that begins as the glass gets in contact with physiological fluids and this release is believed to stimulate osteogenesis. There is evidence that the ionic products of BG enhance osteoblast attachment, proliferation, differentiation and mineralization, and also induce differentiation of bone marrow stromal cells (4).…”

Section: Introductionmentioning

confidence: 99%

Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

et al. 2017

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This study aimed to investigate the influence of a three-dimensional cell culture model and bioactive glass (BG) particles on the expression of osteoblastic phenotypes in rat calvaria osteogenic cells culture. Cells were seeded on two-dimensional (2D) and threedimensional (3D) collagen with BG particles for up to 14 days. Cell viability and alkaline phosphatase (ALP) activity was performed. Cell morphology and immunolabeling of noncollagenous bone matrix proteins were assessed by epifluorescence and confocal microscopy. The expressions of osteogenic markers were analyzed using RT-PCR. Mineralized bone-like nodule formation was visualized by microscopy and calcium content was assessed quantitatively by alizarin red assay. Experimental cultures produced a growing cell viability rate up to 14 days. Although ALP activity at 7 days was higher on BG cultures, cells on 3D and 3D+BG had an activity decrease of ALP at 14 days. Three-dimensional conditions favored the immunolabeling for OPN and BSP and the expression of ALP and COL I mRNAs. BG particles influenced positively the OC and OPN mRNAs expression and calcified nodule formation in vitro. The results indicated that the 3D cultures and BG particles contribute to the expression of osteoblastic phenotype and to differentiated and mineralized matrix formation.

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Section: Introductionmentioning

confidence: 99%

Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

et al. 2017

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“…By contrast, in-vitro, it has been documented that the ionic products from the dissolution of the bioactive glass actually enhance osteoblast attachment, proliferation, differentiation and mineralization [10][11][12], as well as induce the differentiation of bone marrow stromal cells into mature extracellular producing osteoblasts [13]. Furthermore, the dissolution products of bioactive glass exert control over genetic factors of bone growth [14].…”

Section: Introductionmentioning

confidence: 99%

Effect of ciprofloxacin incorporation in PVA and PVA bioactive glass composite scaffolds

Mabrouk

Mostafa

Oudadesse

et al. 2014

Ceramics International

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Scaffolds are implants used to deliver cells, drugs, and genes into the body in a local controlled release pattern which offers many advantages over systematic drug delivery. Composite scaffolds of polyvinyl alcohol (PVA) and quaternary bioactive glass (46S6 system) with different ratios of glass contents were prepared by lyophilisation technique. The broad spectrum antibiotic ciprofloxacin (Cip) was impregnated to the scaffold during the fabrication in a concentration of 5, 10 and 20%. Biodegradation rate and in-vitro mineralization of the prepared scaffolds were performed by soaking the scaffolds in simulated body fluid (SBF). Phase identification, microstructure, porosity, bioactivity, mechanical properties and drug release pattern in PBS were characterized by XRD, SEM coupled with EDS, Hg-porosimeter, inductively coupled plasma-optical emission spectroscopy (ICP-OES), universal testing machine, fourier transform infrared (FTIR) and UV-spectrophotometer, respectively. A porous scaffold has been obtained with porosity up to 85%. By increasing the glass contents in the prepared scaffold the porosity and the degradation rate decrease however, the compressive strength was enhanced. A sustained drug release pattern was observed with a quasi-Fickian diffusion mechanism. The formulated ciprofloxacin loaded porous polyvinyl alcohol scaffold gave an acceptable physicochemical properties and was able to deliver the drug in a prolonged release pattern which offers a distinguish treatment for osteomylitis as well as local antibacterial effect.

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“…[17][18][19][20] Calcium phosphate scaffolds mimic bone minerals and can facilitate cell attachment and function. 14,[21][22][23] They are bioactive and can bond to bone to form a functional interface. 8,18,24,25 Calcium phosphate cements (CPCs) have good biocompatibility and osteoconductivity.…”

Section: Introductionmentioning

confidence: 99%

Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells

Chen

Zhou

Tang

et al. 2012

Tissue Engineering Part A

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Tissue engineering approaches are promising to meet the increasing need for bone regeneration. Calcium phosphate cement (CPC) can be injected and self-set to form a scaffold with excellent osteoconductivity. The objectives of this study were to develop a macroporous CPC-chitosan-fiber construct containing alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem cells (hUCMSCs) and to investigate hUCMSC release from the degrading microbeads and proliferation inside the porous CPC construct. The hUCMSC-encapsulated microbeads were completely wrapped inside the CPC paste, with the gas-foaming porogen creating macropores in CPC to provide for access to culture media. Increasing the porogen content in CPC significantly increased the cell viability, from 49% of live cells in CPC with 0% porogen to 86% of live cells in CPC with 15% porogen. The alginate-fibrin microbeads started to degrade and release the cells inside CPC at 7 days. The released cells started to proliferate inside the macroporous CPC construct. The live cell number inside CPC increased from 270 cells/mm 2 at 1 day to 350 cells/mm 2 at 21 days. The pore volume fraction of CPC increased from 46.8% to 78.4% using the gas-foaming method, with macropore sizes of approximately 100 to 400 mm. The strength of the CPC-chitosan-fiber scaffold at 15% porogen was 3.8 MPa, which approximated the reported 3.5 MPa for cancellous bone. In conclusion, a novel gas-foaming macroporous CPC construct containing degradable alginate-fibrin microbeads was developed that encapsulated hUCMSCs. The cells had good viability while wrapped inside the porous CPC construct. The degradable microbeads in CPC quickly released the cells, which proliferated over time inside the porous CPC. Self-setting, strong CPC with alginate-fibrin microbeads for stem cell delivery is promising for bone tissue engineering applications.

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Osteogenic effects of bioactive glass on bone marrow stromal cells

Cited by 87 publications

References 28 publications

Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

Bioactive Glass Particles in Two-Dimensional and Three-Dimensional Osteogenic Cell Cultures

Effect of ciprofloxacin incorporation in PVA and PVA bioactive glass composite scaffolds

Gas-Foaming Calcium Phosphate Cement Scaffold Encapsulating Human Umbilical Cord Stem Cells

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