Ototoxicity and Drug Transport in the Cochlea

Kennon-McGill, Stefanie; McGill, Mitchell R.

doi:10.1002/9781119171003.ch13

Cited by 1 publication

(1 citation statement)

References 53 publications

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“…[42][43][44] Most of the studies have used >5 mm of acetaminophen to assess the hepatotoxicity of the drug. [45][46][47] The acute toxicity of acetaminophen on hepatic cells, both primary hepatocytes within the Generation 3 oLOCs and HepG2 cells within the Generation 2 oLOCs, was assessed by measuring the viability and metabolic activity of hepatocytes perfused with different concentrations of acetaminophen for 48 h. Hepatotoxicity of acetaminophen on primary hepatocytes is presented in Figure 4a as live/ dead staining images. Similarly, Figure S5a of the Supporting Information shows the cytotoxicity (live/dead staining) of acetaminophen on HepG2 cells.…”

Section: Wwwadvmattechnoldementioning

confidence: 99%

Customizable Microfluidic Origami Liver‐on‐a‐Chip (oLOC)

Xie

Maharjan

Kelly

et al. 2021

Adv Materials Technologies

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The design and manufacture of an origami‐based liver‐on‐a‐chip device is presented, together with demonstrations of the chip's effectiveness at recapitulating some of the liver's key in vivo architecture, physical microenvironment, and functions. Laser‐cut layers of polyimide tape are folded together with polycarbonate nanoporous membranes to create a stack of three adjacent flow chambers separated by the membranes. Endothelial cells are seeded in the upper and lower flow chambers to simulate sinusoids, and hepatocytes are seeded in the middle flow chamber. Nutrients and metabolites flow through the simulated sinusoids and diffuse between the vascular pathways and the hepatocyte layers, mimicking physiological microcirculation. Studies of cell viability, metabolic functions, and hepatotoxicity of pharmaceutical compounds show that the endothelialized liver‐on‐a‐chip model is conducive to maintaining hepatocyte functions and evaluation of the hepatotoxicity of drugs. The unique origami approach speeds chip development and optimization, effectively simplifying the laboratory‐scale fabrication of on‐chip models of human tissues without necessarily reducing their structural and functional sophistication.

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