Outer Membrane Vesicles Protect Gram-Negative Bacteria against Host Defense Peptides

Balhuizen, Melanie D.; Dijk, Albert van; Jansen, Jeroen W. A.; Lest, Chris H.A. van de; Veldhuizen, Edwin J.A.; Haagsman, Henk P.

doi:10.1128/msphere.00523-21

Cited by 31 publications

(33 citation statements)

References 48 publications

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“…For instance, differentially packaged sRNAs in H. pyroli-derived OMVs attenuated IL-8 secretion in human cells to evade the host immune response [51]. Furthermore, OMVs could protect Gram-negative bacteria against host defense peptides (HDPs), part of the innate immune system, and could comprise first-line defense against invading pathogens [76].…”

Section: Immunomodulatory Host-bacteria Communicationsmentioning

confidence: 99%

Biomimetic Nanoparticles Coated with Bacterial Outer Membrane Vesicles as a New-Generation Platform for Biomedical Applications

et al. 2021

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The biomedical field is currently reaping the benefits of research on biomimetic nanoparticles (NPs), which are synthetic nanoparticles fabricated with natural cellular materials for nature-inspired biomedical applications. These camouflage NPs are capable of retaining not only the physiochemical properties of synthetic nanoparticles but also the original biological functions of the cellular materials. Accordingly, NPs coated with cell-derived membrane components have achieved remarkable growth as prospective biomedical materials. Particularly, bacterial outer membrane vesicle (OMV), which is a cell membrane coating material for NPs, is regarded as an important molecule that can be employed in several biomedical applications, including immune response activation, cancer therapeutics, and treatment for bacterial infections with photothermal activity. The currently available cell membrane-coated NPs are summarized in this review. Furthermore, the general features of bacterial OMVs and several multifunctional NPs that could serve as inner core materials in the coating strategy are presented, and several methods that can be used to prepare OMV-coated NPs (OMV-NPs) and their characterization are highlighted. Finally, some perspectives of OMV-NPs in various biomedical applications for future potential breakthrough are discussed. This in-depth review, which includes potential challenges, will encourage researchers to fabricate innovative and improvised, new-generation biomimetic materials through future biomedical applications.

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Section: Immunomodulatory Host-bacteria Communicationsmentioning

confidence: 99%

Biomimetic Nanoparticles Coated with Bacterial Outer Membrane Vesicles as a New-Generation Platform for Biomedical Applications

et al. 2021

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“…Furthermore, OMVs can be induced by membrane destabilization using sonication, which does not remove LPS from the membrane [65][66][67]. These vesicles are prepared by sonication of the bacterial pellet, thereby forming membrane fragments which fuse to form lysis OMVs (lOMVs).…”

Section: Induction Methods Of Omvsmentioning

confidence: 99%

“…No binding was observed of CATH-2 with rough LPS of E. coli and this correlated with lower MIC values, while binding was observed with smooth LPS of E. coli, resulting in higher MIC values [64]. The importance of an Oantigen was also demonstrated in Bordetella bronchiseptica, since loss of the O-antigen resulted in increased susceptibility for several cationic peptides, to a similar extent as Bordetella pertussis who intrinsically lacks the O-antigen [65]. Lack of an O-antigen might decrease entrapment of HDPs in this complex sugar network.…”

Section: Lps Bindingmentioning

confidence: 97%

“…B. pertussis lOMVs have been studied first, induced using sonication methods. Additionally, the pagL gene was introduced in this bacterial strain, which removes one acyl chain of the LPS, to decrease LPS toxicity (therefore resulting in lgOMVs) [65].…”

Section: B Pertussismentioning

confidence: 99%

“…Recently, the immune response evoked by B. pertussis lOMVs was studied further and revealed to activate the inflammasome in mice and human macrophages [146]. However, since the lOMVs or lgOMVs were extracted using sonication, which disrupts the entire bacterial membrane, contamination of the OMV sample by other bacterial products could have occurred, or the loss of natural cargo, making the studied immune responses not relevant to in vivo produced sOMVs [65].…”

Section: B Pertussismentioning

confidence: 99%

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Interplay between Outer Membrane Vesicles and Host Defense Peptides

Balhuizen¹

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Gram-negative bacteria release vesicular structures from their outer membrane, so called outer membrane vesicles (OMVs). OMVs have a variety of functions, such as waste disposal, communication and antigen or toxin delivery. These vesicles are promising structures for vaccine development since OMVs carry many surface antigens identical to the bacterial surface. However, isolation is often difficult and results in low yields. Several methods to enhance OMV yield exist, but these do affect the resulting OMVs. In this review, our current knowledge about OMVs will be presented. Different methods to induce OMVs will be reviewed and their advantages and disadvantages will be discussed. The effects of the induction and isolation methods used in several immunological studies on OMVs will be compared. Finally, the challenges for OMVbased vaccine development will be examined and one example of a successful OMVbased vaccine will be presented.

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Outer Membrane Vesicle‐Coated Nanoparticle Vaccine Protects against Acinetobacter baumannii Pneumonia and Sepsis

Bjånes

Zhou

Qayum

et al. 2022

Advanced NanoBiomed Research

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The highly multidrug‐resistant (MDR) Gram‐negative bacterial pathogen Acinetobacter baumannii is a top global health priority where an effective vaccine can protect susceptible populations and limit resistance acquisition. Outer membrane vesicles (OMVs) shed from Gram‐negative bacteria are enriched with virulence factors and membrane lipids but heterogeneous in size and cargo. Herein, a vaccine platform combining precise and replicable nanoparticle technology with immunogenic A. baumannii OMVs (Ab‐OMVs) is reported. Gold nanoparticle cores coated with Ab‐OMVs (Ab‐NPs) induce robust IgG titers in rabbits that enhance human neutrophil opsonophagocytic killing and passively protect against lethal A. baumannii sepsis in mice. Active Ab‐NP immunization in mice protects against sepsis and pneumonia, accompanied by B cell recruitment to draining lymph nodes, activation of dendritic cell markers, improved splenic neutrophil responses, and mitigation of proinflammatory cytokine storm. Nanoparticles are an efficient and efficacious platform for OMV vaccine delivery against A. baumannii and perhaps other high‐priority MDR pathogens.

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Outer Membrane Vesicles Protect Gram-Negative Bacteria against Host Defense Peptides

Cited by 31 publications

References 48 publications

Biomimetic Nanoparticles Coated with Bacterial Outer Membrane Vesicles as a New-Generation Platform for Biomedical Applications

Biomimetic Nanoparticles Coated with Bacterial Outer Membrane Vesicles as a New-Generation Platform for Biomedical Applications

Interplay between Outer Membrane Vesicles and Host Defense Peptides

Outer Membrane Vesicle‐Coated Nanoparticle Vaccine Protects against Acinetobacter baumannii Pneumonia and Sepsis

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