Ovarian Dendritic Cells Act as a Double-Edged Pro-Ovulatory and Anti-Inflammatory Sword

Cohen-Fredarow, Adva; Tadmor, Ari; Raz, Tal; Meterani, Naama; Addadi, Yoseph; Nevo, Nava; Solomonov, Inna; Sagi, Irit; Mor, Gil; Neeman, Michal; Dekel, Nava

doi:10.1210/me.2013-1400

Cited by 37 publications

(28 citation statements)

References 80 publications

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“…Moreover, in the process of follicle rupture, there is enhanced ovarian blood flow and participation of proteinases, bradykinins, and histamine, which are all features of acute inflammatory reaction. Furthermore, the involvement of immune cells such as dendritic cells was also demonstrated in the ovulatory process (Cohen-Fredarow et al 2014). …”

Section: Cumulus Expansionmentioning

confidence: 99%

Ovarian Folliculogenesis

Rimon-Dahari¹,

Yerushalmi-Heinemann²,

Alyagor³

et al. 2016

Results and Problems in Cell Differentiation

Self Cite

195

111

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Gosipol is a substances contained in extracted cotton seed which is thought to have the antifertility ability therefore it is often used as a herbal contraceptive. The aim of this study were to assess the folliculogenesis in mice after administrated with cottonseed extract. 60 female mice strain DDY which was 14-15 weeks old and 30-35 g body weight were divided into five groups and given cottonseed extract each 0; 1,5; 2,1 and 2,7 g/kg BW for 5, 10, 15, 24, and 24 + 10 days (without cottonseed treatment). At the end of the treatment period, mice was euthanasia to observe follicular development histomorphology (each three mice of each treatment). Mice estrous status were evaluated based on the description of the vaginal smear cells with Giemsa staining. The results showed that the number of developing follicles was low (P < 0.05) compared with control after 5 days cottonseed extract administration at dose 2,7 g/kg BW that were 23 ± 3,6. At dose 1,5 and 2,1 g/kg BW the number of follicles was low after 24 days that were 25 ± 10,4 and 27 ± 3,5. Recovery effects of follicle number after cottonseed extract administration for 24 days was the best at a dose of 1,5 g/kg BW. Prolonge of estrous cycle occured in mice which were administrated the cottonseed extract of at all dose treatment. In conclusion, although the decrease in the number of developing follicles and prolonge of estrous cycles occurred after cottonseed extract administration, but these effects are reversible after the administration ended. Abstrak Gosipol adalah zat yang terkandung dalam ekstrak biji kapas dan diduga mempunyai kemampuan antifertilitas sehingga sering digunakan sebagai bahan herbal kontrasepsi. Penelitian ini bertujuan mengkaji gambaran folikulogenesis pada mencit yang diberi ekstrak biji kapas. Sebanyak 60 ekor induk mencit betina strain DDY berumur 14-15 minggu dengan bobot badan 30-35 g dibagi menjadi 4 kelompok dan diberi ekstrak biji kapas masing-masing 0; 1,5; 2,1 dan 2,7 g/kg BB selama 5, 10, 15, 24 dan 24 + 10 hari (tanpa pemberian ekstrak biji kapas). Pada setiap akhir waktu pengamatan, mencit di-euthanasia untuk mengamati histomorfologi perkembangan folikel (masing-masing tiga ekor dari tiap perlakuan). Status estrus mencit dievaluasi berdasarkan gambaran sel dari ulas vagina dengan pewarnaan Giemsa. Hasil penelitian menunjukkan bahwa jumlah folikel rendah (P < 0,05) dibandingkan dengan kontrol setelah 5 hari pemberian ekstrak biji kapas dengan dosis 2,7 g/kg BB yaitu 23 ± 3,6. Pada dosis 1,5 dan 2,1 g/kg BB jumlah folikel menjadi rendah setelah 24 hari yaitu 25 ± 10,4; 27 ± 3,5. Efek pemulihan jumlah folikel setelah pemberian ekstrak biji kapas selama 24 hari paling baik pada dosis 1,5 g/kg BB. Pemanjangan siklus estrus terjadi pada mencit yang diberi ekstrak bji kapas pada semua dosis perlakuan. Berdasarkan hasil tersebut dapat disimpulkan bahwa walaupun terjadi penurunan jumlah folikel yang berkembang dan terjadi pemanjangan siklus estrus setelah pemberian ekstrak biji kapas, tetapi efek tersebut bersifat reversible setelah pe...

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Section: Cumulus Expansionmentioning

confidence: 99%

Ovarian Folliculogenesis

Rimon-Dahari¹,

Yerushalmi-Heinemann²,

Alyagor³

et al. 2016

Results and Problems in Cell Differentiation

Self Cite

195

111

View full text Add to dashboard Cite

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“…Four-week-old mice were administered 5 IU PMSG followed by injection of 7 IU hCG (22). At 2 hours after hCG injection, mice were anesthetized, and one of their ovaries was exteriorized via a small dorsal incision.…”

Section: Intrabursal Injectionmentioning

confidence: 99%

A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation

et al. 2015

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Granulosa cells support the developing oocytes and serve as transducers of the ovulatory stimulus induced by LH surge. Fyn kinase is expressed in granulosa cells, though its role in these cells has not been studied. In human embryonic kidney 293T cells, microRNA (miR)-125a-3p down-regulates Fyn expression, causing a decrease in cells' migratory ability. Our aim was to explore the role of miR-125a-3p and Fyn in granulosa cells toward ovulation, focusing on migration as a possible mechanism. We demonstrate expression of miR-125a-3p and Fyn in mouse mural granulosa cells of preovulatory follicles and miR125a-3p-induced down-regulation of Fyn expression in a granulosa cell line (rat). Administration of human chorionic gonadotropin (hCG; LH analog) caused a 75% decrease in the in vivo miR-125a-3p:Fyn mRNA ratio, followed by a 2-fold increased migratory ability of mural granulosa cells. In the hCG-treated granulosa cell line, miR-125a-3p expression was decreased, followed by Fyn up-regulation and phosphorylation of focal adhesion kinase and paxillin, enabling cell migration. An in vivo interference with miR125a-3p:Fyn mRNA ratio in granulosa cells by intrabursal injections of Fyn small interfering RNA or miR-125a-3p mimic caused a 33 or 55% decrease in the number of ovulated oocytes, respectively. These observations reveal a new regulatory pathway in mural granulosa cells under the regulation of LH/hCG. Modulation of cell migration may account for the significance of the LH/hCG-miR-125a-3p-Fyn pathway to ovulation.-Grossman, H., Chuderland, D., Ninio-Many, L., Hasky, N., Kaplan-Kraicer, R., Shalgi, R. A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation. FASEB J. 29, 3206-3216 (2015). www.fasebj.org

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“…Since the major population of CD11c-positive cells described thus far is the F4/80-expressing macrophages [21], for a higher level of resolution, sorted CD45-positive, CD11c-positive, F4/80-negative cells from mouse kidneys were further analysed for expression of the two DC maturation markers MHCII and CD80. To demonstrate the effect of DIPC on kidney DCs, we obtained renal cells from animals 24 h after a second I/R procedure and examined the immune phenotypes.…”

Section: Resultsmentioning

confidence: 99%

Delayed Ischemic Preconditioning Attenuated Renal Ischemia-Reperfusion Injury by Inhibiting Dendritic Cell Maturation

Zhang

Song

Fang

et al. 2018

Cell Physiol Biochem

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Background/Aims: Even though delayed ischemic preconditioning (DIPC) has been reported to produce renal protection, the underlying mechanism remains poorly understood. We reported that a 15-minute renal ischemic preconditioning (IPC) 4 days before subsequent ischemia-reperfusion attenuated renal injury Kidney dendritic cells (DCs) are abundant in the renal tubulointerstitium and, depending on their status, can induce immune activation or tolerance. The aim of the present study was to investigate the role of DCs in IPC of the kidney. Methods: Mouse kidneys were challenged by transient brief episodes of sublethal ischemia followed by subsequent prolonged ischemia. DC abundance and maturation in the spleen and kidney were measured by flow cytometry and immunohistochemical staining. To confirm the function of mature DCs in the renoprotective effect of IPC on renal ischemia-reperfusion injury the A2 adenosine receptor (A2AR) antagonist SCH58261 was administered to stimulate DC maturation prior to assessment of renal functional and histological injury and the inflammatory reaction. Results: Compared with sham-operated animals, preconditioned mice had a reduced injury with less CD11c⁺ cells, lower levels of the pro-inflammatory cytokine IL-17 and reduced expression of the mature DC marker CCR7. Preconditioned mice also produced more of the anti-inflammatory cytokine IL-10. Both renal cells and splenocytes from these mice had more DCs (CD45⁺/CD11c⁺/F4/80^-), but fewer of these DCs were mature (CD45⁺/CD11c⁺/ F4/80^-/MHC-II⁺/CD80⁺) compared with those from sham-treated animals, suggesting that the immunomodulatory effect of renal ischemic preconditioning is both local and systemic. Additionally, injection of the A2AR antagonist SCH58261 reversed IPC-induced inhibition of DC maturation and mitigated the protective effect of preconditioning, suggesting that DC maturation contributes to immune cell-mediated ischemic preconditioning. Conclusion: Our results show that DIPC of the kidney provides local and systemic immunosuppression by inhibiting DC maturation and hence mediates a renal protective effect.

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Ovarian Dendritic Cells Act as a Double-Edged Pro-Ovulatory and Anti-Inflammatory Sword

Cited by 37 publications

References 80 publications

Ovarian Folliculogenesis

Ovarian Folliculogenesis

A novel regulatory pathway in granulosa cells, the LH/human chorionic gonadotropin-microRNA-125a-3p-Fyn pathway, is required for ovulation

Delayed Ischemic Preconditioning Attenuated Renal Ischemia-Reperfusion Injury by Inhibiting Dendritic Cell Maturation

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