Ovarian tissue and follicle transplantation as an option for fertility preservation

Ovarian tissue cryopreservation in girls undergoing haematopoietic stem cell transplant: experience of a single centre

“…The amount of cortex to be cut is a compromise between the need to cryopreserve as much tissue as possible and the need to maintain an ovarian volume permitting future transplantation: usually about 50% of ovarian cortex was removed. 17 After retrieval, the ovarian tissue was immediately rinsed in in vitro fertilization (IVF) buffered medium and transported on ice to the IVF laboratory, where the freezing procedure took place. In our centre, rapid transport of the removed tissue to the laboratory was performed.…”

Methods and conditions of fertility preservation in early-stage ovarian tumors

“…Az egészséges petefészkek megőrzésére alternatív megoldást biztosíthatnak a jelenleg még kezdeti fázisban lévő újabb eljárások, úgymint a petefészek-cryopraeservatio, majd reimplantáció [30,31,32,33,34]. Petefészek-cryopraeservatio 14-40 éves korú nőknél jön szóba, akik olyan onkológiai kezelésben kell, hogy része-süljenek, amely a petefészkeket károsíthatja [35].…”

In Vitro Culture of Early Secondary Preantral Follicles in Hanging Drop of Ovarian Cell-Conditioned Medium to Obtain MII Oocytes from Outbred Deer Mice

“…[6][7][8][9][10][11] Although orthotopic transplantation of the banked ovarian tissue has been shown to produce offspring in both mice and humans, it also contains the risk of reintroducing bloodborne (in ovarian tissue) malignant cells into the patients with cancer. [12][13][14][15] An alternative way that is attracting more and more attention is to isolate preantral follicles from fresh or banked ovarian tissue for in vitro culture to obtain mature oocytes (the various stages of follicle and oocyte development are explained in Supplementary Fig. S1; Supplementary Data are available online at www.liebertpub.com/tea).…”