Over‐expression of a single Ca<sup>2+</sup>‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants

Saijo, Yusuke; Hata, Shingo; Kyozuka, Junko; Shimamoto, Ko; Izui, Katsura

doi:10.1046/j.1365-313x.2000.00787.x

Cited by 794 publications

(511 citation statements)

References 64 publications

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“…These osmotic stress signals induce a calcium-signaling response in plants (Knight et al, 1997). Part of the response involves an increase in the expression of CDPKs (Urao et al, 1994), which are among the calcium-signaling targets involved in salt and drought stress adaptation (Sheen, 1996;Saijo et al, 2000). MIP proteins are subject to regulation by drought and salinity, but this regulation usually is exhibited by changes in gene expression, leading to alterations in the levels or localization of plant MIP proteins (Johansson et al, 2000;Maurel et al, 2002;Tyerman et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

et al. 2003

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Soybean nodulin 26 is expressed and targeted to the symbiosome membrane of nitrogen-fixing nodules, where it forms an aquaporin channel with a modest water transport rate. In this study, we show that the phosphorylation of nodulin 26 on Ser-262, which is catalyzed by a symbiosome membrane-associated calcium-dependent protein kinase, stimulates its intrinsic water transport rate. Furthermore, using a phosphospecific antibody, we have elucidated the developmental appearance and regulation of nodulin 26 phosphorylation in vivo. Although nodulin 26 protein is detected first in differentiating infected cells (16 days), phosphorylated nodulin 26 does not become pronounced until infected cell maturation (25 days). Phosphorylation is sustained at steady state levels until entry into senescence. Nodulin 26 phosphorylation is enhanced further by osmotic stresses (water deprivation and salinity). Thus, the phosphorylation of nodulin 26 coincides with the establishment of mature nitrogen-fixing symbiosomes, is regulated by osmotic stresses that induce calcium-signaling pathways, and appears to be part of the adaptive responses of infected cells to osmotic challenge.

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Section: Discussionmentioning

confidence: 99%

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

et al. 2003

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“…Expression of CDPKs from other plants is affected by a variety of stimuli including wounding (Chico et al, 2002), salt or drought stress (Botella et al, 1996;Patharkar and Cushman, 2000;Saijo et al, 2000), cold (Monroy and Dhindsa, 1995;Saijo et al, 2000), hormone treatment (Abo-El-Saad and Wu, 1995;Botella et al, 1996;Davletova et al, 2001), light (Frattini et al, 1999), cold (Martin and Busconi, 2001;Llop-Tous et al, 2002), and pathogens (Murillo et al, 2001;Romeis et al, 2001). Specific expression patterns have been described for CDPKs in many plant species including maize (Zea mays) pollen (Estruch et al, 1994), rice vascular tissue (Saijo et al, 2001) and seeds (Kawasaki et al, 1993;Frattini et al, 1999), and potato (Solanum tuberosum) stolons (Raices et al, 2001).…”

Section: Cdpkmentioning

confidence: 99%

The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases

Hrabak

Chan²,

Gribskov³

et al. 2003

Plant Physiology

908

823

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The CDPK-SnRK superfamily consists of seven types of serine-threonine protein kinases: calcium-dependent protein kinase (CDPKs), CDPK-related kinases (CRKs), phosphoenolpyruvate carboxylase kinases (PPCKs), PEP carboxylase kinase-related kinases (PEPRKs), calmodulin-dependent protein kinases (CaMKs), calcium and calmodulin-dependent protein kinases (CCaMKs), and SnRKs. Within this superfamily, individual isoforms and subfamilies contain distinct regulatory domains, subcellular targeting information, and substrate specificities. Our analysis of the Arabidopsis genome identified 34 CDPKs, eight CRKs, two PPCKs, two PEPRKs, and 38 SnRKs. No definitive examples were found for a CCaMK similar to those previously identified in lily (Lilium longiflorum) and tobacco (Nicotiana tabacum) or for a CaMK similar to those in animals or yeast. CDPKs are present in plants and a specific subgroup of protists, but CRKs, PPCKs, PEPRKs, and two of the SnRK subgroups have been found only in plants. CDPKs and at least one SnRK have been implicated in decoding calcium signals in Arabidopsis. Analysis of intron placements supports the hypothesis that CDPKs, CRKs, PPCKs and PEPRKs have a common evolutionary origin; however there are no conserved intron positions between these kinases and the SnRK subgroup. CDPKs and SnRKs are found on all five Arabidopsis chromosomes. The presence of closely related kinases in regions of the genome known to have arisen by genome duplication indicates that these kinases probably arose by divergence from common ancestors. The PlantsP database provides a resource of continuously updated information on protein kinases from Arabidopsis and other plants.In eukaryotes, protein kinases are involved in regulating key aspects of cellular function, including cell division, metabolism, and responses to external signals. The completed sequence of the Arabidopsis genome provides the first opportunity to identify all of the protein kinases present in a model plant. The Arabidopsis genome encodes 1,085 typical protein kinases (M. Gribskov, unpublished data), which is about 4% of the predicted 25,500 genes (Arabidopsis Article, publication date, and citation information can be found at www.plantphysiol.org/cgi

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“…The [Ca 2+ ]cyt signatures elicited by one environmental challenge can be modified by prior exposure to a contrasting one (Knight, 2000). Furthermore, transformants obtained via manipulation of one or more components of Ca 2+ signaling pathway were observed to be more tolerant to adverse stress conditions, especially water deficit (Saijo et al, 2000, Pandey et al, 2002, Cheong et al, 2003,, Wang et al, 2005, Liu et al, 2006, Ma et al, 2007.…”

Section: Calcium and Its Rolementioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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Over‐expression of a single Ca²⁺‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants

Cited by 794 publications

References 64 publications

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

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Over‐expression of a single Ca2+‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants

Cited by 794 publications

References 64 publications

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

Phosphorylation of Soybean Nodulin 26 on Serine 262 Enhances Water Permeability and Is Regulated Developmentally and by Osmotic Signals

The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

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Product

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Over‐expression of a single Ca²⁺‐dependent protein kinase confers both cold and salt/drought tolerance on rice plants