Overcoming Matrix Effects in Liquid Chromatography−Mass Spectrometry

Cappiello, Achille; Famiglini, Giorgio; Pierini, Elisabetta; Termopoli, Veronica; Trufelli, Helga

doi:10.1021/ac8018312

Cited by 232 publications

(134 citation statements)

References 36 publications

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“…DEI has been shown to be less prone to matrix effects for direct mixture analyses [30]. When coupled with CP-MIMS, for complex samples, we observe better results (e.g., less signal reduction) than typically obtained using ESI ionization [5,7].…”

Section: Resultsmentioning

confidence: 52%

“…Typically, relatively simple mass spectra are obtained, with intense molecular ion signals [25]. Several researchers have been developing approaches to ionize trace level analytes directly from liquids using EI, including molecular beam based interfaces by the Amirav group [26,27] and direct EI (DEI) by the Cappiello group [28][29][30]. With DEI, a low flow of liquid carrier is admitted directly to a heated EI source under high vacuum.…”

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confidence: 99%

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Condensed Phase Membrane Introduction Mass Spectrometry with Direct Electron Ionization: On-line Measurement of PAHs in Complex Aqueous Samples

Termopoli

Famiglini

Palma

et al. 2015

J. Am. Soc. Mass Spectrom.

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Abstract. Polycyclic aromatic hydrocarbons (PAHs) are USEPA regulated priority pollutants. Their low aqueous solubility requires very sensitive analytical methods for their detection, typically involving preconcentration steps. Presented is the first demonstrated 'proof of concept' use of condensed phase membrane introduction mass spectrometry (CP-MIMS) coupled with direct liquid electron ionization (DEI) for the direct, on-line measurement of PAHs in aqueous samples. DEI is very well suited for the ionization of PAHs and other nonpolar compounds, and is not significantly influenced by the co-elution of matrix components. Linear calibration data for low ppb levels of aqueous naphthalene, anthracene, and pyrene is demonstrated, with measured detection limits of 4 ppb. Analytical response times (t 10%-90% signal rise) ranged from 2.8 min for naphthalene to 4.7 min for pyrene. Both intra-and interday reproducibility has been assessed (<3% and 5% RSD, respectively). Direct measurements of ppb level PAHs spiked in a variety of real, complex environmental sample matrices is examined, including natural waters, sea waters, and a hydrocarbon extraction production waste water sample. For these spiked, complex samples, direct PAH measurement by CP-MIMS-DEI yielded minimal signal suppression from sample matrix effects (81%-104%). We demonstrate the use of this analytical approach to directly monitor real-time changes in aqueous PAH concentrations with potential applications for continuous on-line monitoring strategies and binding/adsorption studies in heterogeneous samples.

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Section: Resultsmentioning

confidence: 52%

mentioning

confidence: 99%

Condensed Phase Membrane Introduction Mass Spectrometry with Direct Electron Ionization: On-line Measurement of PAHs in Complex Aqueous Samples

Termopoli

Famiglini

Palma

et al. 2015

J. Am. Soc. Mass Spectrom.

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“…On one hand, chlorogenic acid was overestimated with the UV-PDA detector compared to the MS quantification (slope value: 1.345). Matrix effects are generally associated with response suppression effects, as observed in ESI-MS quantification, when compared to other detection methods [18,21]. Under our conditions, a co-elution phenomenon probably explains the UV/MS differences in chlorogenic acid estimations [22].…”

Section: Comparison Of the Uhplc-uv And Uhplc-ms/ms Methodsmentioning

confidence: 64%

Comparison of Two Methods, UHPLC-UV and UHPLC-MS/MS, for the Quantification of Polyphenols in Cider Apple Juices

et al. 2013

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Abstract:The aim of this study was to develop faster and more efficient phenotyping methods for in-depth genetic studies on cider apple progeny. The UHPLC chromatographic system was chosen to separate polyphenolic compounds, and quantifications were then simultaneously performed with a UV-PDA detector and an ESI-triple quadrupole mass analyzer (SRM mode). Both quantification methods were validated for 15 major compounds using two apple juice samples, on the basis of linearity, limits of detection and quantification, recovery and precision tests. The comparison between UV and SRM quantifications in 120 different samples of a cider apple progeny showed an excellent correlation for major compounds quantified with both methods. However, an overestimation was revealed for five compounds with the UV detector and the mass analyzer. Co-elution and matrix effects are discussed to explain this phenomenon. SRM methods should therefore be considered with restrictions in some cases for quantification measurements when several phenolic compounds are simultaneously quantified in complex matrices such as apple juices. For both methods, analyses were carried out over short periods of time while maintaining a high quality for the simultaneous quantification of OPEN ACCESSMolecules 2013, 18 10214 phenolic compounds in apple juice. Each method is relevant for more in-depth genetic studies of the polyphenol content of apple juice.

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“…The main culprit of these occurrences is the complexity of the soils [24,57,58]. This phenomenon is called matrix effect (ME); it is highly compounddependent and can involve either an unexpected suppression or enhancement of the analyte response induced by the coeluting matrix [57][58][59][60]. Most of the compounds susceptible to matrix-induced enhancement are polar, capable of strong hydrogen-bonding, acids or bases [58].…”

Section: Matrix Effectmentioning

confidence: 99%